Tags

Type your tag names separated by a space and hit enter

New flaxseed orbitides: Detection, sequencing, and (15)N incorporation.
Biopolymers. 2014 Mar; 102(2):168-75.B

Abstract

Three new orbitides (cyclolinopeptides 17, 18, and 19) were identified in flaxseed (Linum usitatissimum L.) extracts without any form of purification. Their structures were elucidated by a combination of (15) N-labeling experiments and extensive tandem mass spectrometry (MS/MS) with electrospray ionization (ESI). Putative linear peptide sequences of the new orbitides were used as the query in the Basic Local Alignment Search Tool (BLAST) searches of flax genome database. These searches returned linear sequences for the putative precursors of cyclolinopeptides 17 and 19 among others. Cyclolinopeptide 18 contains MetO (O) and is not directly encoded, but is a product of post-translation modification of the Met present in 17. The identification of precursor proteins in flax mRNA transcripts and DNA sequences confirmed the occurrence and amino acid sequences of these orbitides as [1-9-NαC]-MLKPFFFWI, [1-9-NαC]-OLKPFFFWI, and [1-9-NαC]-GIPPFWLTL for cyclolinopeptides 17, 18, and 19, respectively.

Authors+Show Affiliations

Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24408479

Citation

Okinyo-Owiti, Denis P., et al. "New Flaxseed Orbitides: Detection, Sequencing, and (15)N Incorporation." Biopolymers, vol. 102, no. 2, 2014, pp. 168-75.
Okinyo-Owiti DP, Young L, Burnett PG, et al. New flaxseed orbitides: Detection, sequencing, and (15)N incorporation. Biopolymers. 2014;102(2):168-75.
Okinyo-Owiti, D. P., Young, L., Burnett, P. G., & Reaney, M. J. (2014). New flaxseed orbitides: Detection, sequencing, and (15)N incorporation. Biopolymers, 102(2), 168-75. https://doi.org/10.1002/bip.22459
Okinyo-Owiti DP, et al. New Flaxseed Orbitides: Detection, Sequencing, and (15)N Incorporation. Biopolymers. 2014;102(2):168-75. PubMed PMID: 24408479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New flaxseed orbitides: Detection, sequencing, and (15)N incorporation. AU - Okinyo-Owiti,Denis P, AU - Young,Lester, AU - Burnett,Peta-Gaye G, AU - Reaney,Martin J T, PY - 2013/09/10/received PY - 2013/12/20/revised PY - 2013/12/23/accepted PY - 2014/1/11/entrez PY - 2014/1/11/pubmed PY - 2016/5/31/medline KW - cyclic peptides KW - flax KW - mass spectrometry KW - orbitides KW - sequencing SP - 168 EP - 75 JF - Biopolymers JO - Biopolymers VL - 102 IS - 2 N2 - Three new orbitides (cyclolinopeptides 17, 18, and 19) were identified in flaxseed (Linum usitatissimum L.) extracts without any form of purification. Their structures were elucidated by a combination of (15) N-labeling experiments and extensive tandem mass spectrometry (MS/MS) with electrospray ionization (ESI). Putative linear peptide sequences of the new orbitides were used as the query in the Basic Local Alignment Search Tool (BLAST) searches of flax genome database. These searches returned linear sequences for the putative precursors of cyclolinopeptides 17 and 19 among others. Cyclolinopeptide 18 contains MetO (O) and is not directly encoded, but is a product of post-translation modification of the Met present in 17. The identification of precursor proteins in flax mRNA transcripts and DNA sequences confirmed the occurrence and amino acid sequences of these orbitides as [1-9-NαC]-MLKPFFFWI, [1-9-NαC]-OLKPFFFWI, and [1-9-NαC]-GIPPFWLTL for cyclolinopeptides 17, 18, and 19, respectively. SN - 1097-0282 UR - https://www.unboundmedicine.com/medline/citation/24408479/New_flaxseed_orbitides:_Detection_sequencing_and__15_N_incorporation_ L2 - https://doi.org/10.1002/bip.22459 DB - PRIME DP - Unbound Medicine ER -