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The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death.
Phytomedicine. 2014 Apr 15; 21(5):724-33.P

Abstract

Liuwei dihuang (LWDH), a widely used traditional Chinese medicine (TCM), has been employed as an anti-aging prescription to improve declined function. Parkinson's disease (PD) is a common adult-onset neurodegenerative disorder characterized by the degeneration of dopaminergic nigrostriatal neurons with complex pathological mechanisms, including oxidative stress. Increasing evidence indicate that TCM has the potential to be neuroprotective drugs because of their antioxidant characteristics. The aim of this study is to investigate the mechanisms of LWDH-mediated protection in Parkinson's toxin-induced dopaminergic neurodegeneration by evaluating water extract of LWDH (LWDH-WE) in 1-methyl-4-phenylpyridinium (MPP(+))-treated primary mesencephalic neurons and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated C57BL/6 mice. In the present study, chemical profiling and quantitative analysis of LWDH-WE were revealed using 3D-HPLC technique, and were confirmed by the data of three batches of LWDH-WE. In primary mesencephalic neuronal cultures, LWDH-WE decreased MPP(+)-induced loss of tyrosine hydroxylase (TH)-positive neurons and increase of Annexin V-positive neurons. LWDH-WE reduced MPP(+)-induced oxidative damage via increasing antioxidant defense (SOD, GSH), decreasing ROS production, and down-regulating NADPH oxidases (Nox2 and Nox4). Also, LWDH-WE inhibited neuronal apoptosis by improving mitochondrial membrane potential, increasing antiapoptotic protein Bcl-2 expression, and down-regulating apoptotic signaling (Bax, cytochrome c, cleaved-caspase-3) in MPP(+)-treated neurons. In MPTP-treated C57BL/6 mice, LWDH-WE attenuated TH-positive neuronal loss in substantia nigra pars compacta (SNpc), and improved locomotor activity of mice. In conclusion, the present results reveal that LWDH-WE possesses protection on dopaminergic neurons through enhancing antioxidant defense and decreasing apoptotic death, suggesting the potential benefits of LWDH-WE for PD treatment.

Authors+Show Affiliations

Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Department of Pharmacology, School of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address: yichlo@kmu.edu.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24411708

Citation

Tseng, Yu-Ting, et al. "The Chinese Herbal Formula Liuwei Dihuang Protects Dopaminergic Neurons Against Parkinson's Toxin Through Enhancing Antioxidative Defense and Preventing Apoptotic Death." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 21, no. 5, 2014, pp. 724-33.
Tseng YT, Chang FR, Lo YC. The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death. Phytomedicine. 2014;21(5):724-33.
Tseng, Y. T., Chang, F. R., & Lo, Y. C. (2014). The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 21(5), 724-33. https://doi.org/10.1016/j.phymed.2013.11.001
Tseng YT, Chang FR, Lo YC. The Chinese Herbal Formula Liuwei Dihuang Protects Dopaminergic Neurons Against Parkinson's Toxin Through Enhancing Antioxidative Defense and Preventing Apoptotic Death. Phytomedicine. 2014 Apr 15;21(5):724-33. PubMed PMID: 24411708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death. AU - Tseng,Yu-Ting, AU - Chang,Fang-Rong, AU - Lo,Yi-Ching, Y1 - 2014/01/07/ PY - 2013/10/09/received PY - 2013/11/14/accepted PY - 2014/1/14/entrez PY - 2014/1/15/pubmed PY - 2014/12/17/medline KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - Dopaminergic neurons KW - Liuwei dihuang KW - Neuroprotection KW - Traditional Chinese medicine SP - 724 EP - 33 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 21 IS - 5 N2 - Liuwei dihuang (LWDH), a widely used traditional Chinese medicine (TCM), has been employed as an anti-aging prescription to improve declined function. Parkinson's disease (PD) is a common adult-onset neurodegenerative disorder characterized by the degeneration of dopaminergic nigrostriatal neurons with complex pathological mechanisms, including oxidative stress. Increasing evidence indicate that TCM has the potential to be neuroprotective drugs because of their antioxidant characteristics. The aim of this study is to investigate the mechanisms of LWDH-mediated protection in Parkinson's toxin-induced dopaminergic neurodegeneration by evaluating water extract of LWDH (LWDH-WE) in 1-methyl-4-phenylpyridinium (MPP(+))-treated primary mesencephalic neurons and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated C57BL/6 mice. In the present study, chemical profiling and quantitative analysis of LWDH-WE were revealed using 3D-HPLC technique, and were confirmed by the data of three batches of LWDH-WE. In primary mesencephalic neuronal cultures, LWDH-WE decreased MPP(+)-induced loss of tyrosine hydroxylase (TH)-positive neurons and increase of Annexin V-positive neurons. LWDH-WE reduced MPP(+)-induced oxidative damage via increasing antioxidant defense (SOD, GSH), decreasing ROS production, and down-regulating NADPH oxidases (Nox2 and Nox4). Also, LWDH-WE inhibited neuronal apoptosis by improving mitochondrial membrane potential, increasing antiapoptotic protein Bcl-2 expression, and down-regulating apoptotic signaling (Bax, cytochrome c, cleaved-caspase-3) in MPP(+)-treated neurons. In MPTP-treated C57BL/6 mice, LWDH-WE attenuated TH-positive neuronal loss in substantia nigra pars compacta (SNpc), and improved locomotor activity of mice. In conclusion, the present results reveal that LWDH-WE possesses protection on dopaminergic neurons through enhancing antioxidant defense and decreasing apoptotic death, suggesting the potential benefits of LWDH-WE for PD treatment. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/24411708/The_Chinese_herbal_formula_Liuwei_dihuang_protects_dopaminergic_neurons_against_Parkinson's_toxin_through_enhancing_antioxidative_defense_and_preventing_apoptotic_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(13)00467-4 DB - PRIME DP - Unbound Medicine ER -