Tags

Type your tag names separated by a space and hit enter

Modulation of blood oxylipin levels by long-chain omega-3 fatty acid supplementation in hyper- and normolipidemic men.
Prostaglandins Leukot Essent Fatty Acids 2014 Feb-Mar; 90(2-3):27-37PL

Abstract

INTRODUCTION

Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) such as EPA and DHA have been shown to possess beneficial health effects, and it is believed that many of their effects are mediated by their oxygenated products (oxylipins). Recently, we have shown that serum levels of several hydroxy, epoxy, and dihydroxy FAs are dependent on the individual status of the parent FAs in a cohort of normo- and hyperlipidemic subjects. So far, the effect of an increased dietary LC n-3 PUFA intake on hydroxy, epoxy, and dihydroxy FA levels has not been investigated in subjects with mild combined hyperlipidemia.

SUBJECTS AND METHODS

In the present study, we compared oxylipin patterns of 10 hyperlipidemic (cholesterol >200mg/dl; triglyceride >150mg/ml) and 10 normolipidemic men in response to twelve weeks of LC n-3 PUFA intake (1.14g DHA and 1.56g EPA). Levels of 44 free hydroxy, epoxy and dihydroxy FAs were analyzed in serum by LC-MS. Additionally, oxylipin levels were compared with their parent PUFA levels in erythrocyte membranes; a biomarker for the individual PUFA status.

RESULTS

Differences in the oxylipin pattern between normo- and hyperlipidemic subjects were minor before and after treatment. In all subjects, levels of EPA-derived oxylipins (170-4800pM) were considerably elevated after LC n-3 PUFA intake (150-1400%), the increase of DHA-derived oxylipins (360-3900pM) was less pronounced (30-130%). The relative change of EPA in erythrocyte membranes is strongly correlated (r≥0.5; p<0.05) with the relative change of corresponding epoxy and dihydroxy FA serum levels. The effect on arachidonic acid (AA)-derived oxylipin levels (140-27,100pM) was inconsistent.

DISCUSSION AND CONCLUSIONS

The dietary LC PUFA composition has a direct influence on the endogenous oxylipin profile, including several highly biological active EPA- and DHA-derived lipid mediators. The shift in oxylipin pattern appears to be dependent on the initial LC PUFA status particularly for EPA. The finding that also levels of other oxylipins derived from ALA, LA or AA are modified by LC n-3 PUFA intake might suggest that at least some of the effects of EPA and DHA could be mediated by a shift in the entire oxylipin profile.

Authors+Show Affiliations

Institute of Food Science and Human Nutrition, Leibniz University Hannover, Germany. Electronic address: Schuchardt@nutrition.uni-hannover.de.Institute of Food Science and Human Nutrition, Leibniz University Hannover, Germany.Institute of Food Science and Human Nutrition, Leibniz University Hannover, Germany.Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Germany.Department of Entomology and Comprehensive Cancer Center, University of California Davis, CA, USA.Institute of Food Science and Human Nutrition, Leibniz University Hannover, Germany.Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Germany.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24411718

Citation

Schuchardt, Jan Philipp, et al. "Modulation of Blood Oxylipin Levels By Long-chain Omega-3 Fatty Acid Supplementation in Hyper- and Normolipidemic Men." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 90, no. 2-3, 2014, pp. 27-37.
Schuchardt JP, Schmidt S, Kressel G, et al. Modulation of blood oxylipin levels by long-chain omega-3 fatty acid supplementation in hyper- and normolipidemic men. Prostaglandins Leukot Essent Fatty Acids. 2014;90(2-3):27-37.
Schuchardt, J. P., Schmidt, S., Kressel, G., Willenberg, I., Hammock, B. D., Hahn, A., & Schebb, N. H. (2014). Modulation of blood oxylipin levels by long-chain omega-3 fatty acid supplementation in hyper- and normolipidemic men. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 90(2-3), pp. 27-37. doi:10.1016/j.plefa.2013.12.008.
Schuchardt JP, et al. Modulation of Blood Oxylipin Levels By Long-chain Omega-3 Fatty Acid Supplementation in Hyper- and Normolipidemic Men. Prostaglandins Leukot Essent Fatty Acids. 2014;90(2-3):27-37. PubMed PMID: 24411718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of blood oxylipin levels by long-chain omega-3 fatty acid supplementation in hyper- and normolipidemic men. AU - Schuchardt,Jan Philipp, AU - Schmidt,Simone, AU - Kressel,Gaby, AU - Willenberg,Ina, AU - Hammock,Bruce D, AU - Hahn,Andreas, AU - Schebb,Nils Helge, Y1 - 2013/12/27/ PY - 2013/08/01/received PY - 2013/12/19/revised PY - 2013/12/19/accepted PY - 2014/1/14/entrez PY - 2014/1/15/pubmed PY - 2014/10/10/medline KW - Arachidonic acid KW - DHA KW - Diols KW - EPA KW - Eicosanoids KW - Epoxides KW - Hyperlipidemia KW - Omega-3 fatty acids KW - PUFA SP - 27 EP - 37 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 90 IS - 2-3 N2 - INTRODUCTION: Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) such as EPA and DHA have been shown to possess beneficial health effects, and it is believed that many of their effects are mediated by their oxygenated products (oxylipins). Recently, we have shown that serum levels of several hydroxy, epoxy, and dihydroxy FAs are dependent on the individual status of the parent FAs in a cohort of normo- and hyperlipidemic subjects. So far, the effect of an increased dietary LC n-3 PUFA intake on hydroxy, epoxy, and dihydroxy FA levels has not been investigated in subjects with mild combined hyperlipidemia. SUBJECTS AND METHODS: In the present study, we compared oxylipin patterns of 10 hyperlipidemic (cholesterol >200mg/dl; triglyceride >150mg/ml) and 10 normolipidemic men in response to twelve weeks of LC n-3 PUFA intake (1.14g DHA and 1.56g EPA). Levels of 44 free hydroxy, epoxy and dihydroxy FAs were analyzed in serum by LC-MS. Additionally, oxylipin levels were compared with their parent PUFA levels in erythrocyte membranes; a biomarker for the individual PUFA status. RESULTS: Differences in the oxylipin pattern between normo- and hyperlipidemic subjects were minor before and after treatment. In all subjects, levels of EPA-derived oxylipins (170-4800pM) were considerably elevated after LC n-3 PUFA intake (150-1400%), the increase of DHA-derived oxylipins (360-3900pM) was less pronounced (30-130%). The relative change of EPA in erythrocyte membranes is strongly correlated (r≥0.5; p<0.05) with the relative change of corresponding epoxy and dihydroxy FA serum levels. The effect on arachidonic acid (AA)-derived oxylipin levels (140-27,100pM) was inconsistent. DISCUSSION AND CONCLUSIONS: The dietary LC PUFA composition has a direct influence on the endogenous oxylipin profile, including several highly biological active EPA- and DHA-derived lipid mediators. The shift in oxylipin pattern appears to be dependent on the initial LC PUFA status particularly for EPA. The finding that also levels of other oxylipins derived from ALA, LA or AA are modified by LC n-3 PUFA intake might suggest that at least some of the effects of EPA and DHA could be mediated by a shift in the entire oxylipin profile. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/24411718/Modulation_of_blood_oxylipin_levels_by_long_chain_omega_3_fatty_acid_supplementation_in_hyper__and_normolipidemic_men_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(13)00249-4 DB - PRIME DP - Unbound Medicine ER -