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Characteristic development of the GABA-removal system in the mouse spinal cord.
Neuroscience 2014; 262:129-42N

Abstract

GABA is a predominant inhibitory neurotransmitter in the CNS. Released GABA is removed from the synaptic cleft by two GABA transporters (GATs), GAT-1 and GAT-3, and their dysfunction affects brain functions. The present study aimed to reveal the ontogeny of the GABA-removal system by examining the immunohistochemical localization of GAT-1 and GAT-3 in the embryonic and postnatal mouse cervical spinal cord. In the dorsal horn, GAT-1 was localized within the presynapses of inhibitory axons after embryonic day 15 (E15), a little prior to GABAergic synapse formation. The GAT-1-positive dots increased in density until postnatal day 21 (P21). By contrast, in the ventral horn, GAT-1-positive dots were sparse during development, although many transient GABAergic synapses were formed before birth. GAT-3 was first localized within the radial processes of radial glia in the ventral part on E12 and the dorsal part on E15. The initial localization of the GAT-3 was almost concomitant with the dispersal of GABAergic neurons. GAT-3 continued to be localized within the processes of astrocytes, and increased in expression until P21. These results suggested the following: (1) before synapse formation, GABA may be transported into the processes of radial glia or immature astrocytes by GAT-3. (2) At the transient GABAergic synapses in the ventral horn, GABA may not be reuptaken into the presynapses. (3) In the dorsal horn, GABA may start to be reuptaken by GAT-1 a little prior to synapse formation. (4) After synapse formation, GAT-3 may continue to remove GABA from immature and mature synaptic clefts into the processes of astrocytes. (5) Development of the GABA-removal system may be completed by P21.

Authors+Show Affiliations

Department of Molecular Anatomy, School of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 9030215, Japan.Department of Molecular Anatomy, School of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 9030215, Japan.Department of Molecular Anatomy, School of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 9030215, Japan.Department of Molecular Anatomy, School of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 9030215, Japan.Department of Molecular Anatomy, School of Medicine, University of the Ryukyus, Uehara 207, Nishihara, Okinawa 9030215, Japan. Electronic address: takachan@med.u-ryukyu.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24412234

Citation

Kim, J, et al. "Characteristic Development of the GABA-removal System in the Mouse Spinal Cord." Neuroscience, vol. 262, 2014, pp. 129-42.
Kim J, Kosaka Y, Shimizu-Okabe C, et al. Characteristic development of the GABA-removal system in the mouse spinal cord. Neuroscience. 2014;262:129-42.
Kim, J., Kosaka, Y., Shimizu-Okabe, C., Niizaki, A., & Takayama, C. (2014). Characteristic development of the GABA-removal system in the mouse spinal cord. Neuroscience, 262, pp. 129-42. doi:10.1016/j.neuroscience.2013.12.066.
Kim J, et al. Characteristic Development of the GABA-removal System in the Mouse Spinal Cord. Neuroscience. 2014 Mar 14;262:129-42. PubMed PMID: 24412234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristic development of the GABA-removal system in the mouse spinal cord. AU - Kim,J, AU - Kosaka,Y, AU - Shimizu-Okabe,C, AU - Niizaki,A, AU - Takayama,C, Y1 - 2014/01/09/ PY - 2013/07/25/received PY - 2013/12/28/revised PY - 2013/12/31/accepted PY - 2014/1/14/entrez PY - 2014/1/15/pubmed PY - 2014/12/15/medline KW - GABA transporter 1 (GAT-1) KW - GABA transporter 3 (GAT-3) KW - GABAergic synapse KW - astrocyte KW - radial glia KW - vesicular GABA transporter (VGAT) SP - 129 EP - 42 JF - Neuroscience JO - Neuroscience VL - 262 N2 - GABA is a predominant inhibitory neurotransmitter in the CNS. Released GABA is removed from the synaptic cleft by two GABA transporters (GATs), GAT-1 and GAT-3, and their dysfunction affects brain functions. The present study aimed to reveal the ontogeny of the GABA-removal system by examining the immunohistochemical localization of GAT-1 and GAT-3 in the embryonic and postnatal mouse cervical spinal cord. In the dorsal horn, GAT-1 was localized within the presynapses of inhibitory axons after embryonic day 15 (E15), a little prior to GABAergic synapse formation. The GAT-1-positive dots increased in density until postnatal day 21 (P21). By contrast, in the ventral horn, GAT-1-positive dots were sparse during development, although many transient GABAergic synapses were formed before birth. GAT-3 was first localized within the radial processes of radial glia in the ventral part on E12 and the dorsal part on E15. The initial localization of the GAT-3 was almost concomitant with the dispersal of GABAergic neurons. GAT-3 continued to be localized within the processes of astrocytes, and increased in expression until P21. These results suggested the following: (1) before synapse formation, GABA may be transported into the processes of radial glia or immature astrocytes by GAT-3. (2) At the transient GABAergic synapses in the ventral horn, GABA may not be reuptaken into the presynapses. (3) In the dorsal horn, GABA may start to be reuptaken by GAT-1 a little prior to synapse formation. (4) After synapse formation, GAT-3 may continue to remove GABA from immature and mature synaptic clefts into the processes of astrocytes. (5) Development of the GABA-removal system may be completed by P21. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/24412234/Characteristic_development_of_the_GABA_removal_system_in_the_mouse_spinal_cord_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(14)00004-9 DB - PRIME DP - Unbound Medicine ER -