Tags

Type your tag names separated by a space and hit enter

Bioactive Lignans from Zanthoxylum alatum Roxb. stem bark with cytotoxic potential.
J Ethnopharmacol 2014; 152(1):106-12JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Zanthoxylum alatum is used in traditional medicinal systems for number of disorders like cholera, diabetes, cough, diarrhea, fever, headache, microbial infections, toothache, inflammation and cancer. The aim of the present study was to evaluate Zanthoxylum alatum stem bark for its cytotoxic potential and to isolate the bioactive constituents.

MATERIAL AND METHODS

Cytotoxicity of the different extracts and isolated compounds was studied on lung carcinoma cell line (A549) and pancreatic carcinoma cell line (MIA-PaCa) using MTT assay. Isolation of compounds from most active extract (petroleum ether) was done on silica gel column. Structure elucidation was done by using various spectrophotometric techniques like UV, IR, (1)H NMR, (13)C NMR and mass spectroscopy. The type of cell death caused by most active compound C was explored by fluorescence microscopy using the acridine orange/ethidium bromide method.

RESULT

Petroleum ether extract of plant has shown significant cytotoxic potential. Three lignans sesamin (A), kobusin (B), and 4'O demethyl magnolin (C) has been isolated. All lignans showed cytotoxic activities in different ranges. Compound C was the novel bioactive compound from a plant source and found to be most active. In apoptosis study, treatment caused typical apoptotic morphological changes. It enhances the apoptosis at IC50 dose (21.72 µg/mL) however showing necrotic cell death at higher dose after 24h on MIA-PaCa cell lines.

CONCLUSION

Petroleum ether extract (60-80 °C) of Zanthoxylum alatum has cytotoxic potential. The lignans isolated from the petroleum ether extract were responsible for the cytotoxic potential of the extract. 4'O demethyl magnolin was novel compound from Zanthoxylum alatum. Hence the Zanthoxylum alatum can be further explored for the development of anticancer drug.

Authors+Show Affiliations

Punjab Technical University, Kapurthala, India; ISF College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga 142001 Punjab, India.ISF College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga 142001 Punjab, India.ISF College of Pharmacy, Ferozepur Road, Ghal Kalan, Moga 142001 Punjab, India. Electronic address: ankalia_47@rediffmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24412550

Citation

Mukhija, Minky, et al. "Bioactive Lignans From Zanthoxylum Alatum Roxb. Stem Bark With Cytotoxic Potential." Journal of Ethnopharmacology, vol. 152, no. 1, 2014, pp. 106-12.
Mukhija M, Lal Dhar K, Nath Kalia A. Bioactive Lignans from Zanthoxylum alatum Roxb. stem bark with cytotoxic potential. J Ethnopharmacol. 2014;152(1):106-12.
Mukhija, M., Lal Dhar, K., & Nath Kalia, A. (2014). Bioactive Lignans from Zanthoxylum alatum Roxb. stem bark with cytotoxic potential. Journal of Ethnopharmacology, 152(1), pp. 106-12. doi:10.1016/j.jep.2013.12.039.
Mukhija M, Lal Dhar K, Nath Kalia A. Bioactive Lignans From Zanthoxylum Alatum Roxb. Stem Bark With Cytotoxic Potential. J Ethnopharmacol. 2014 Feb 27;152(1):106-12. PubMed PMID: 24412550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive Lignans from Zanthoxylum alatum Roxb. stem bark with cytotoxic potential. AU - Mukhija,Minky, AU - Lal Dhar,Kanaya, AU - Nath Kalia,Ajudhia, Y1 - 2014/01/08/ PY - 2013/07/20/received PY - 2013/11/30/revised PY - 2013/12/21/accepted PY - 2014/1/14/entrez PY - 2014/1/15/pubmed PY - 2014/10/7/medline KW - Column chromatography KW - Cytotoxicity KW - Lignans KW - MTT assay KW - Zanthoxylum alatum SP - 106 EP - 12 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 152 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Zanthoxylum alatum is used in traditional medicinal systems for number of disorders like cholera, diabetes, cough, diarrhea, fever, headache, microbial infections, toothache, inflammation and cancer. The aim of the present study was to evaluate Zanthoxylum alatum stem bark for its cytotoxic potential and to isolate the bioactive constituents. MATERIAL AND METHODS: Cytotoxicity of the different extracts and isolated compounds was studied on lung carcinoma cell line (A549) and pancreatic carcinoma cell line (MIA-PaCa) using MTT assay. Isolation of compounds from most active extract (petroleum ether) was done on silica gel column. Structure elucidation was done by using various spectrophotometric techniques like UV, IR, (1)H NMR, (13)C NMR and mass spectroscopy. The type of cell death caused by most active compound C was explored by fluorescence microscopy using the acridine orange/ethidium bromide method. RESULT: Petroleum ether extract of plant has shown significant cytotoxic potential. Three lignans sesamin (A), kobusin (B), and 4'O demethyl magnolin (C) has been isolated. All lignans showed cytotoxic activities in different ranges. Compound C was the novel bioactive compound from a plant source and found to be most active. In apoptosis study, treatment caused typical apoptotic morphological changes. It enhances the apoptosis at IC50 dose (21.72 µg/mL) however showing necrotic cell death at higher dose after 24h on MIA-PaCa cell lines. CONCLUSION: Petroleum ether extract (60-80 °C) of Zanthoxylum alatum has cytotoxic potential. The lignans isolated from the petroleum ether extract were responsible for the cytotoxic potential of the extract. 4'O demethyl magnolin was novel compound from Zanthoxylum alatum. Hence the Zanthoxylum alatum can be further explored for the development of anticancer drug. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24412550/Bioactive_Lignans_from_Zanthoxylum_alatum_Roxb__stem_bark_with_cytotoxic_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00921-5 DB - PRIME DP - Unbound Medicine ER -