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SERS-based direct and sandwich assay methods for mir-21 detection.
Analyst. 2014 Mar 07; 139(5):1141-7.A

Abstract

In this study, two different assay methods were developed using a surface enhanced Raman scattering (SERS) label for sensitive miR-21 detection. In the first method (direct assay), the miR-21 probe was attached to SERS-labelled, rod-shaped gold nanoparticles and hybridised with the target miR-21, which was previously immobilised onto the gold slide. In the second method (sandwich assay), the target miR-21 was captured by an miR-21 probe immobilised onto the gold slide and hybridised with a second miR-21 probe immobilised on the SERS-labeled, rod-shaped gold nanoparticles. SERS signals of developed assays were obtained via a SERS spectrum of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) on the rod-shaped nanoparticles. The calibration curves were plotted to measure the different concentrations of miR-21. The detection limits of the direct and sandwich assays, which last less than 40 min, were found to be 0.36 and 0.85 nM, respectively. The developed SERS-based methods offer rapid, selective, sensitive and easy detection of miR-21, especially compared to conventional PCR-based methods.

Authors+Show Affiliations

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey. ihb@hacettepe.edu.tr.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24418951

Citation

Guven, Burcu, et al. "SERS-based Direct and Sandwich Assay Methods for Mir-21 Detection." The Analyst, vol. 139, no. 5, 2014, pp. 1141-7.
Guven B, Dudak FC, Boyaci IH, et al. SERS-based direct and sandwich assay methods for mir-21 detection. Analyst. 2014;139(5):1141-7.
Guven, B., Dudak, F. C., Boyaci, I. H., Tamer, U., & Ozsoz, M. (2014). SERS-based direct and sandwich assay methods for mir-21 detection. The Analyst, 139(5), 1141-7. https://doi.org/10.1039/c3an01600e
Guven B, et al. SERS-based Direct and Sandwich Assay Methods for Mir-21 Detection. Analyst. 2014 Mar 7;139(5):1141-7. PubMed PMID: 24418951.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SERS-based direct and sandwich assay methods for mir-21 detection. AU - Guven,Burcu, AU - Dudak,Fahriye Ceyda, AU - Boyaci,Ismail Hakki, AU - Tamer,Ugur, AU - Ozsoz,Mehmet, PY - 2014/1/15/entrez PY - 2014/1/15/pubmed PY - 2014/8/29/medline SP - 1141 EP - 7 JF - The Analyst JO - Analyst VL - 139 IS - 5 N2 - In this study, two different assay methods were developed using a surface enhanced Raman scattering (SERS) label for sensitive miR-21 detection. In the first method (direct assay), the miR-21 probe was attached to SERS-labelled, rod-shaped gold nanoparticles and hybridised with the target miR-21, which was previously immobilised onto the gold slide. In the second method (sandwich assay), the target miR-21 was captured by an miR-21 probe immobilised onto the gold slide and hybridised with a second miR-21 probe immobilised on the SERS-labeled, rod-shaped gold nanoparticles. SERS signals of developed assays were obtained via a SERS spectrum of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) on the rod-shaped nanoparticles. The calibration curves were plotted to measure the different concentrations of miR-21. The detection limits of the direct and sandwich assays, which last less than 40 min, were found to be 0.36 and 0.85 nM, respectively. The developed SERS-based methods offer rapid, selective, sensitive and easy detection of miR-21, especially compared to conventional PCR-based methods. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/24418951/SERS_based_direct_and_sandwich_assay_methods_for_mir_21_detection_ DB - PRIME DP - Unbound Medicine ER -