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Comparison of antigen specificity, class II major histocompatibility complex restriction, and in vivo behavior of myelin basic protein-specific T cell lines and clones derived from (BALB/c x SJL/J) mice.
J Immunol. 1987 Sep 15; 139(6):1834-9.JI

Abstract

Immunization with myelin basic protein (BP) causes experimental allergic encephalomyelitis (EAE) in certain strains of mice. SJL/J (H-2s) is the prototype sensitive strain. Although BALB/c (H-2d) is resistant to EAE through use of an identical immunization protocol, (BALB/c x SJL/J)F1 hybrid mice develop EAE after immunization with BP. T cell clones specific for BP have been isolated from a highly encephalitogenic line of (BALB/c x SJL/J)F1 hybrid T cells raised against bovine BP. The clones were examined for their H-2 restriction and specificity for heterologous forms of BP (mouse, rat, and bovine BP). The results revealed the clones cross-reacting with mouse (self) BP were almost always restricted to F1 hybrid class II major histocompatibility complex (MHC) elements. In contrast, mouse cross-reactive clones derived from a nonencephalitogenic (BALB/c x SJL/J) T cell line raised against rat BP were largely restricted to H-2d elements. These clones did not cross-react with bovine BP. Four additional lines were generated by carrying the original rat and bovine F1 T cell lines on parental antigen-presenting cells thus generating lines biased toward homozygous (SJL/J, H-2s, or BALB/c, H-2d) restriction elements. These "parentally restricted" T cell lines did not induce EAE when injected in vivo. These results suggest that in this F1 strain sensitivity to T cell-induced EAE is associated with epitopes on murine BP that associate with F1 class II MHC restricting elements. In contrast, nonencephalitogenic T cell lines contain a high proportion of murine cross-reactive clones restricted to H-2d, the haplotype of the classically resistant BALB/c mouse. This work illustrates the use of T cell lines and clones in a model system to further analyze the role of MHC restriction elements in autoimmune disease occurring in heterozygous individuals.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2442257

Citation

Trotter, J, et al. "Comparison of Antigen Specificity, Class II Major Histocompatibility Complex Restriction, and in Vivo Behavior of Myelin Basic Protein-specific T Cell Lines and Clones Derived From (BALB/c X SJL/J) Mice." Journal of Immunology (Baltimore, Md. : 1950), vol. 139, no. 6, 1987, pp. 1834-9.
Trotter J, Zamvil SS, Steinman L. Comparison of antigen specificity, class II major histocompatibility complex restriction, and in vivo behavior of myelin basic protein-specific T cell lines and clones derived from (BALB/c x SJL/J) mice. J Immunol. 1987;139(6):1834-9.
Trotter, J., Zamvil, S. S., & Steinman, L. (1987). Comparison of antigen specificity, class II major histocompatibility complex restriction, and in vivo behavior of myelin basic protein-specific T cell lines and clones derived from (BALB/c x SJL/J) mice. Journal of Immunology (Baltimore, Md. : 1950), 139(6), 1834-9.
Trotter J, Zamvil SS, Steinman L. Comparison of Antigen Specificity, Class II Major Histocompatibility Complex Restriction, and in Vivo Behavior of Myelin Basic Protein-specific T Cell Lines and Clones Derived From (BALB/c X SJL/J) Mice. J Immunol. 1987 Sep 15;139(6):1834-9. PubMed PMID: 2442257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of antigen specificity, class II major histocompatibility complex restriction, and in vivo behavior of myelin basic protein-specific T cell lines and clones derived from (BALB/c x SJL/J) mice. AU - Trotter,J, AU - Zamvil,S S, AU - Steinman,L, PY - 1987/9/15/pubmed PY - 1987/9/15/medline PY - 1987/9/15/entrez SP - 1834 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 139 IS - 6 N2 - Immunization with myelin basic protein (BP) causes experimental allergic encephalomyelitis (EAE) in certain strains of mice. SJL/J (H-2s) is the prototype sensitive strain. Although BALB/c (H-2d) is resistant to EAE through use of an identical immunization protocol, (BALB/c x SJL/J)F1 hybrid mice develop EAE after immunization with BP. T cell clones specific for BP have been isolated from a highly encephalitogenic line of (BALB/c x SJL/J)F1 hybrid T cells raised against bovine BP. The clones were examined for their H-2 restriction and specificity for heterologous forms of BP (mouse, rat, and bovine BP). The results revealed the clones cross-reacting with mouse (self) BP were almost always restricted to F1 hybrid class II major histocompatibility complex (MHC) elements. In contrast, mouse cross-reactive clones derived from a nonencephalitogenic (BALB/c x SJL/J) T cell line raised against rat BP were largely restricted to H-2d elements. These clones did not cross-react with bovine BP. Four additional lines were generated by carrying the original rat and bovine F1 T cell lines on parental antigen-presenting cells thus generating lines biased toward homozygous (SJL/J, H-2s, or BALB/c, H-2d) restriction elements. These "parentally restricted" T cell lines did not induce EAE when injected in vivo. These results suggest that in this F1 strain sensitivity to T cell-induced EAE is associated with epitopes on murine BP that associate with F1 class II MHC restricting elements. In contrast, nonencephalitogenic T cell lines contain a high proportion of murine cross-reactive clones restricted to H-2d, the haplotype of the classically resistant BALB/c mouse. This work illustrates the use of T cell lines and clones in a model system to further analyze the role of MHC restriction elements in autoimmune disease occurring in heterozygous individuals. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2442257/Comparison_of_antigen_specificity_class_II_major_histocompatibility_complex_restriction_and_in_vivo_behavior_of_myelin_basic_protein_specific_T_cell_lines_and_clones_derived_from__BALB/c_x_SJL/J__mice_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2442257 DB - PRIME DP - Unbound Medicine ER -