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[Curcumin reduces paraquat-induced oxidative injury in A549 cells by activation of the Nrf2-ARE pathway].

Abstract

OBJECTIVE

To investigate the protective effect of curcumin (CU) on type II alveolar epithelial cells (A549 cells) during paraquat (PQ)-induced oxidative damage and its underlying mechanism.

METHODS

Routinely cultured A549 cells were divided into blank control group, CU control group, PQ group, and PQ+Cu group to receive respective treatments for 24 h. Cell viability was determined by MTT assay. The NFE2L2 expression in A549 cells was measured by RT-PCR and Western blot. The activities of the heme oxygenase-1 (HO-1) and NAD (P) H: quinone oxidoreductase 1 (NQO-1) in cells and the superoxide dismutase (SOD) and catalase (CAT) in supernatant, as well as malondialdehyde (MDA) content, were measured by enzyme-linked immunosorbent assay. After siRNA depletion of Nrf2, the protective effect of CU on A549 cells during PQ-induced oxidative damage was evaluated.

RESULTS

PQ, even at a dose of 0.1 mmol/L, could significantly suppress the viability of A549 cells in a dose-dependent manner. CU showed no significant inhibitory effect on the viability of A549 cells when given at a dose below 160 ümol/L. Compared with the blank control group, the PQ group had significantly decreased SOD activity and significantly increased CAT activity and MDA content after 24-h exposure to 800 ümol/L PQ (P < 0.05 or P < 0.01). Thanks to pretreatment with 80 ümol/L CU, the PQ+CU group had significantly increased SOD and CAT activities and significantly decreased MDA content compared with the PQ group (P < 0.01). Compared with the blank control group, the PQ group had significantly increased expression of NFE2L2 and its downstream factors HO-1 and NQO-1 (P < 0.01), while the PQ+CU group had significantly higher expression of NFE2L2, HO-1,and NQO-1 than the PQ group (P < 0.01).Compared with the PQ+CU group, the CU+PQ+NFE2L2siRNA group had significantly decreased SOD and CAT activities and significantly increased MDA content (P < 0.01).

CONCLUSION

Low-dose CU significantly reduces the PQ-induced oxidative damage in A549 cells in vitro by activation of the Nrf2-ARE pathway.

Authors+Show Affiliations

Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. E-mail: honggl98@126.com.

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

24428990

Citation

Lu, Han, et al. "[Curcumin Reduces Paraquat-induced Oxidative Injury in A549 Cells By Activation of the Nrf2-ARE Pathway]." Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, vol. 32, no. 1, 2014, pp. 44-9.
Lu H, Chang Z, Han W, et al. [Curcumin reduces paraquat-induced oxidative injury in A549 cells by activation of the Nrf2-ARE pathway]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014;32(1):44-9.
Lu, H., Chang, Z., Han, W., Wang, L., & Hong, G. (2014). [Curcumin reduces paraquat-induced oxidative injury in A549 cells by activation of the Nrf2-ARE pathway]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi = Zhonghua Laodong Weisheng Zhiyebing Zazhi = Chinese Journal of Industrial Hygiene and Occupational Diseases, 32(1), 44-9.
Lu H, et al. [Curcumin Reduces Paraquat-induced Oxidative Injury in A549 Cells By Activation of the Nrf2-ARE Pathway]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014;32(1):44-9. PubMed PMID: 24428990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Curcumin reduces paraquat-induced oxidative injury in A549 cells by activation of the Nrf2-ARE pathway]. AU - Lu,Han, AU - Chang,Zijuan, AU - Han,Wenwen, AU - Wang,Lei, AU - Hong,Gungliang, PY - 2014/1/17/entrez PY - 2014/1/17/pubmed PY - 2015/4/10/medline SP - 44 EP - 9 JF - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi VL - 32 IS - 1 N2 - OBJECTIVE: To investigate the protective effect of curcumin (CU) on type II alveolar epithelial cells (A549 cells) during paraquat (PQ)-induced oxidative damage and its underlying mechanism. METHODS: Routinely cultured A549 cells were divided into blank control group, CU control group, PQ group, and PQ+Cu group to receive respective treatments for 24 h. Cell viability was determined by MTT assay. The NFE2L2 expression in A549 cells was measured by RT-PCR and Western blot. The activities of the heme oxygenase-1 (HO-1) and NAD (P) H: quinone oxidoreductase 1 (NQO-1) in cells and the superoxide dismutase (SOD) and catalase (CAT) in supernatant, as well as malondialdehyde (MDA) content, were measured by enzyme-linked immunosorbent assay. After siRNA depletion of Nrf2, the protective effect of CU on A549 cells during PQ-induced oxidative damage was evaluated. RESULTS: PQ, even at a dose of 0.1 mmol/L, could significantly suppress the viability of A549 cells in a dose-dependent manner. CU showed no significant inhibitory effect on the viability of A549 cells when given at a dose below 160 ümol/L. Compared with the blank control group, the PQ group had significantly decreased SOD activity and significantly increased CAT activity and MDA content after 24-h exposure to 800 ümol/L PQ (P < 0.05 or P < 0.01). Thanks to pretreatment with 80 ümol/L CU, the PQ+CU group had significantly increased SOD and CAT activities and significantly decreased MDA content compared with the PQ group (P < 0.01). Compared with the blank control group, the PQ group had significantly increased expression of NFE2L2 and its downstream factors HO-1 and NQO-1 (P < 0.01), while the PQ+CU group had significantly higher expression of NFE2L2, HO-1,and NQO-1 than the PQ group (P < 0.01).Compared with the PQ+CU group, the CU+PQ+NFE2L2siRNA group had significantly decreased SOD and CAT activities and significantly increased MDA content (P < 0.01). CONCLUSION: Low-dose CU significantly reduces the PQ-induced oxidative damage in A549 cells in vitro by activation of the Nrf2-ARE pathway. SN - 1001-9391 UR - https://www.unboundmedicine.com/medline/citation/24428990/[Curcumin_reduces_paraquat_induced_oxidative_injury_in_A549_cells_by_activation_of_the_Nrf2_ARE_pathway]_ DB - PRIME DP - Unbound Medicine ER -