Tags

Type your tag names separated by a space and hit enter

Modulation of oxidative stress and Ca(2+) mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum.
Neuroscience. 2014 Mar 28; 263:27-35.N

Abstract

A main component of St. John's Wort (Hypericum perforatum, HP) is hyperforin which has antioxidant properties in dorsal root ganglion (DRG) neurons, due to its ability to modulate NADPH oxidase and protein kinase C. Recent reports indicate that oxidative stress through NADPH oxidase activates TRPM2 channels. HP may be a useful treatment for Ca(2+) entry and oxidative stress through modulation of TRPM2 channels in the DRG. We aimed to investigate the protective role of HP on Ca(2+) entry and oxidative stress through TRPM2 channels in DRG neurons of rats. The native rat DRG neurons were used in whole-cell patch-clamp, Fura-2 and antioxidant experiments. Appropriate, nontoxic concentrations and incubation times for HP were determined in the DRG neurons by assessing cell viability. The H2O2-induced TRPM2 currents were inhibited by 2-aminoethyl diphenylborinate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA). TRPM2 current densities and cytosolic free Ca(2+) concentration in the neurons were also reduced by HP (2 and 24h). In Fura-2 experiments, cytosolic Ca(2+) mobilization was reduced by voltage-gated calcium channel blockers (verapamil+diltiazem, V+D) and HP. Glutathione peroxidase activity and GSH values in the DRG were high in HP, 2-APB and V+D groups although lipid peroxidation level was low in the groups. In conclusion, we observed a protective role for HP on Ca(2+) entry through a TRPM2 channel in the DRG neurons. Since over-production of oxidative stress and Ca(2+) entry are implicated in the pathophysiology of neuropathic pain and neuronal inflammation, our findings may be relevant to the etiology and treatment of neuropathology in DRG neurons.

Authors+Show Affiliations

Neuroscience Research Center, Süleyman Demirel University, Isparta, Turkey; Department of Biophysics, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey. Electronic address: mnaziroglu@med.sdu.edu.tr.Department of Biophysics, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.Restorative and Regenerative Medicine Research Center, Istanbul Medipol University, Istanbul, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24434769

Citation

Nazıroğlu, M, et al. "Modulation of Oxidative Stress and Ca(2+) Mobilization Through TRPM2 Channels in Rat Dorsal Root Ganglion Neuron By Hypericum Perforatum." Neuroscience, vol. 263, 2014, pp. 27-35.
Nazıroğlu M, Çiğ B, Özgül C. Modulation of oxidative stress and Ca(2+) mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum. Neuroscience. 2014;263:27-35.
Nazıroğlu, M., Çiğ, B., & Özgül, C. (2014). Modulation of oxidative stress and Ca(2+) mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum. Neuroscience, 263, 27-35. https://doi.org/10.1016/j.neuroscience.2014.01.006
Nazıroğlu M, Çiğ B, Özgül C. Modulation of Oxidative Stress and Ca(2+) Mobilization Through TRPM2 Channels in Rat Dorsal Root Ganglion Neuron By Hypericum Perforatum. Neuroscience. 2014 Mar 28;263:27-35. PubMed PMID: 24434769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of oxidative stress and Ca(2+) mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum. AU - Nazıroğlu,M, AU - Çiğ,B, AU - Özgül,C, Y1 - 2014/01/13/ PY - 2013/11/25/received PY - 2014/01/03/revised PY - 2014/01/05/accepted PY - 2014/1/18/entrez PY - 2014/1/18/pubmed PY - 2014/11/2/medline KW - Hypericum perforatum KW - TRPM2 channel antagonists KW - antioxidants KW - glutathione KW - oxidative stress KW - sensory neurons SP - 27 EP - 35 JF - Neuroscience JO - Neuroscience VL - 263 N2 - A main component of St. John's Wort (Hypericum perforatum, HP) is hyperforin which has antioxidant properties in dorsal root ganglion (DRG) neurons, due to its ability to modulate NADPH oxidase and protein kinase C. Recent reports indicate that oxidative stress through NADPH oxidase activates TRPM2 channels. HP may be a useful treatment for Ca(2+) entry and oxidative stress through modulation of TRPM2 channels in the DRG. We aimed to investigate the protective role of HP on Ca(2+) entry and oxidative stress through TRPM2 channels in DRG neurons of rats. The native rat DRG neurons were used in whole-cell patch-clamp, Fura-2 and antioxidant experiments. Appropriate, nontoxic concentrations and incubation times for HP were determined in the DRG neurons by assessing cell viability. The H2O2-induced TRPM2 currents were inhibited by 2-aminoethyl diphenylborinate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA). TRPM2 current densities and cytosolic free Ca(2+) concentration in the neurons were also reduced by HP (2 and 24h). In Fura-2 experiments, cytosolic Ca(2+) mobilization was reduced by voltage-gated calcium channel blockers (verapamil+diltiazem, V+D) and HP. Glutathione peroxidase activity and GSH values in the DRG were high in HP, 2-APB and V+D groups although lipid peroxidation level was low in the groups. In conclusion, we observed a protective role for HP on Ca(2+) entry through a TRPM2 channel in the DRG neurons. Since over-production of oxidative stress and Ca(2+) entry are implicated in the pathophysiology of neuropathic pain and neuronal inflammation, our findings may be relevant to the etiology and treatment of neuropathology in DRG neurons. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/24434769/Modulation_of_oxidative_stress_and_Ca_2+__mobilization_through_TRPM2_channels_in_rat_dorsal_root_ganglion_neuron_by_Hypericum_perforatum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(14)00010-4 DB - PRIME DP - Unbound Medicine ER -