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Comparative structural and functional analysis of genes encoding pectin methylesterases in Phytophthora spp.
Gene. 2014 Mar 15; 538(1):74-83.GENE

Abstract

We have scanned the Phytophthora infestans, P. ramorum, and P. sojae genomes for the presence of putative pectin methylesterase genes and conducted a sequence analysis of all gene models found. We also searched for potential regulatory motifs in the promoter region of the proposed P. infestans models, and investigated the gene expression levels throughout the course of P. infestans infection on potato plants, using in planta and detached leaf assays. We found that genes located on contiguous chromosomal regions contain similar motifs in the promoter region, indicating the possibility of a shared regulatory mechanism. Results of our investigations also suggest that, during the pathogenicity process, the expression levels of some of the analyzed genes vary considerably when compared to basal expression observed in in vitro cultures of non-sporulating mycelium. These results were observed both in planta and in detached leaf assays.

Authors+Show Affiliations

Biology Department, 29 Kunkel Hall, Lafayette College, Easton, PA 18042, USA.Biology Department, 29 Kunkel Hall, Lafayette College, Easton, PA 18042, USA.Biology Department, 29 Kunkel Hall, Lafayette College, Easton, PA 18042, USA. Electronic address: elsgenomics@gmail.com.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24434809

Citation

Mingora, Christina, et al. "Comparative Structural and Functional Analysis of Genes Encoding Pectin Methylesterases in Phytophthora Spp." Gene, vol. 538, no. 1, 2014, pp. 74-83.
Mingora C, Ewer J, Ospina-Giraldo M. Comparative structural and functional analysis of genes encoding pectin methylesterases in Phytophthora spp. Gene. 2014;538(1):74-83.
Mingora, C., Ewer, J., & Ospina-Giraldo, M. (2014). Comparative structural and functional analysis of genes encoding pectin methylesterases in Phytophthora spp. Gene, 538(1), 74-83. https://doi.org/10.1016/j.gene.2014.01.016
Mingora C, Ewer J, Ospina-Giraldo M. Comparative Structural and Functional Analysis of Genes Encoding Pectin Methylesterases in Phytophthora Spp. Gene. 2014 Mar 15;538(1):74-83. PubMed PMID: 24434809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative structural and functional analysis of genes encoding pectin methylesterases in Phytophthora spp. AU - Mingora,Christina, AU - Ewer,Jason, AU - Ospina-Giraldo,Manuel, Y1 - 2014/01/14/ PY - 2013/07/23/received PY - 2013/12/18/revised PY - 2014/01/06/accepted PY - 2014/1/18/entrez PY - 2014/1/18/pubmed PY - 2014/4/12/medline KW - CAZy enzymes KW - Cell wall-degrading enzymes KW - Pectin methylesterases KW - Phytophthora genome KW - Phytophthora infestans KW - Potato KW - Solanum tuberosum SP - 74 EP - 83 JF - Gene JO - Gene VL - 538 IS - 1 N2 - We have scanned the Phytophthora infestans, P. ramorum, and P. sojae genomes for the presence of putative pectin methylesterase genes and conducted a sequence analysis of all gene models found. We also searched for potential regulatory motifs in the promoter region of the proposed P. infestans models, and investigated the gene expression levels throughout the course of P. infestans infection on potato plants, using in planta and detached leaf assays. We found that genes located on contiguous chromosomal regions contain similar motifs in the promoter region, indicating the possibility of a shared regulatory mechanism. Results of our investigations also suggest that, during the pathogenicity process, the expression levels of some of the analyzed genes vary considerably when compared to basal expression observed in in vitro cultures of non-sporulating mycelium. These results were observed both in planta and in detached leaf assays. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24434809/Comparative_structural_and_functional_analysis_of_genes_encoding_pectin_methylesterases_in_Phytophthora_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(14)00037-7 DB - PRIME DP - Unbound Medicine ER -