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Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations.
J Infect Dis. 1987 Nov; 156(5):723-31.JI

Abstract

We studied isolates of nontypable Haemophilus influenzae from cultures of nasopharynx and middle ear fluid (MEF) done simultaneously on children with otitis media. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane protein (OMP) patterns demonstrated that in 16 of 19 pairs, the nasopharyngeal and MEF strains were identical. With four monoclonal antibodies to lipooligosaccharide (LOS) determinants, 17 of the 19 pairs were identical. Thus the pathogenesis of otitis media due to nontypable H. influenzae appears to involve spread of the bacteria from the nasopharynx to the middle ear. Analysis of middle ear isolates from four children with recurrent otitis media caused by nontypable H. influenzae indicated that the recurrent episodes were caused by reinfection with different strains rather than by persistence of the same strain. OMP and LOS analysis of strains from two sisters with concurrent otitis media suggested that person-to-person transmission of nontypable H. influenzae can occur among children.

Authors+Show Affiliations

Department of Medicine, State University of New York at Buffalo.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2443581

Citation

Murphy, T F., et al. "Outer Membrane Protein and Lipooligosaccharide Analysis of Paired Nasopharyngeal and Middle Ear Isolates in Otitis Media Due to Nontypable Haemophilus Influenzae: Pathogenetic and Epidemiological Observations." The Journal of Infectious Diseases, vol. 156, no. 5, 1987, pp. 723-31.
Murphy TF, Bernstein JM, Dryja DM, et al. Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations. J Infect Dis. 1987;156(5):723-31.
Murphy, T. F., Bernstein, J. M., Dryja, D. M., Campagnari, A. A., & Apicella, M. A. (1987). Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations. The Journal of Infectious Diseases, 156(5), 723-31.
Murphy TF, et al. Outer Membrane Protein and Lipooligosaccharide Analysis of Paired Nasopharyngeal and Middle Ear Isolates in Otitis Media Due to Nontypable Haemophilus Influenzae: Pathogenetic and Epidemiological Observations. J Infect Dis. 1987;156(5):723-31. PubMed PMID: 2443581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations. AU - Murphy,T F, AU - Bernstein,J M, AU - Dryja,D M, AU - Campagnari,A A, AU - Apicella,M A, PY - 1987/11/1/pubmed PY - 1987/11/1/medline PY - 1987/11/1/entrez SP - 723 EP - 31 JF - The Journal of infectious diseases JO - J Infect Dis VL - 156 IS - 5 N2 - We studied isolates of nontypable Haemophilus influenzae from cultures of nasopharynx and middle ear fluid (MEF) done simultaneously on children with otitis media. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane protein (OMP) patterns demonstrated that in 16 of 19 pairs, the nasopharyngeal and MEF strains were identical. With four monoclonal antibodies to lipooligosaccharide (LOS) determinants, 17 of the 19 pairs were identical. Thus the pathogenesis of otitis media due to nontypable H. influenzae appears to involve spread of the bacteria from the nasopharynx to the middle ear. Analysis of middle ear isolates from four children with recurrent otitis media caused by nontypable H. influenzae indicated that the recurrent episodes were caused by reinfection with different strains rather than by persistence of the same strain. OMP and LOS analysis of strains from two sisters with concurrent otitis media suggested that person-to-person transmission of nontypable H. influenzae can occur among children. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/2443581/Outer_membrane_protein_and_lipooligosaccharide_analysis_of_paired_nasopharyngeal_and_middle_ear_isolates_in_otitis_media_due_to_nontypable_Haemophilus_influenzae:_pathogenetic_and_epidemiological_observations_ DB - PRIME DP - Unbound Medicine ER -