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Analysis of the physiological control of replication of ColE1-type plasmids.
J Theor Biol 1986; 123(4):453-70JT

Abstract

The physiology of ColE1-type plasmid replication in a growing host has been examined both theoretically, using computer simulation, and experimentally, by observing replication of the plasmid pBR322 after a nutritional shift-up from glycerol minimal medium (doubling time 71 min) to LB medium (doubling time 24 min). The theory was based on a negative control model and uses three rate equations: for the accumulation of cell mass, for the accumulation of the replication inhibitor, and for the rate of plasmid synthesis. The implications of the theory were explored by simulating the effects of changes in the expression of replication control genes. The nutritional shift-up experiment showed that plasmid replication was blocked immediately after the shift for about half a mass doubling time; after that time, replication rapidly increased until plasmid numbers per unit volume of culture parallelled the increase in culture mass. After the establishment of steady-state growth in the post-shift medium, the plasmid concentration (plasmids per cell mass) was reduced in comparison to pre-shift growth in the same proportion as the culture doubling time. The results showed that plasmid replication factors are under metabolic control and that the changes in the control of these factors compensate one another during steady-state growth, but not immediately after the medium shift.

Authors+Show Affiliations

Biology Programs, University of Texas at Dallas, Richardson 75080.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2443764

Citation

Bremer, H, and S Lin-Chao. "Analysis of the Physiological Control of Replication of ColE1-type Plasmids." Journal of Theoretical Biology, vol. 123, no. 4, 1986, pp. 453-70.
Bremer H, Lin-Chao S. Analysis of the physiological control of replication of ColE1-type plasmids. J Theor Biol. 1986;123(4):453-70.
Bremer, H., & Lin-Chao, S. (1986). Analysis of the physiological control of replication of ColE1-type plasmids. Journal of Theoretical Biology, 123(4), pp. 453-70.
Bremer H, Lin-Chao S. Analysis of the Physiological Control of Replication of ColE1-type Plasmids. J Theor Biol. 1986 Dec 21;123(4):453-70. PubMed PMID: 2443764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the physiological control of replication of ColE1-type plasmids. AU - Bremer,H, AU - Lin-Chao,S, PY - 1986/12/21/pubmed PY - 1986/12/21/medline PY - 1986/12/21/entrez SP - 453 EP - 70 JF - Journal of theoretical biology JO - J. Theor. Biol. VL - 123 IS - 4 N2 - The physiology of ColE1-type plasmid replication in a growing host has been examined both theoretically, using computer simulation, and experimentally, by observing replication of the plasmid pBR322 after a nutritional shift-up from glycerol minimal medium (doubling time 71 min) to LB medium (doubling time 24 min). The theory was based on a negative control model and uses three rate equations: for the accumulation of cell mass, for the accumulation of the replication inhibitor, and for the rate of plasmid synthesis. The implications of the theory were explored by simulating the effects of changes in the expression of replication control genes. The nutritional shift-up experiment showed that plasmid replication was blocked immediately after the shift for about half a mass doubling time; after that time, replication rapidly increased until plasmid numbers per unit volume of culture parallelled the increase in culture mass. After the establishment of steady-state growth in the post-shift medium, the plasmid concentration (plasmids per cell mass) was reduced in comparison to pre-shift growth in the same proportion as the culture doubling time. The results showed that plasmid replication factors are under metabolic control and that the changes in the control of these factors compensate one another during steady-state growth, but not immediately after the medium shift. SN - 0022-5193 UR - https://www.unboundmedicine.com/medline/citation/2443764/Analysis_of_the_physiological_control_of_replication_of_ColE1_type_plasmids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-5193(86)80213-2 DB - PRIME DP - Unbound Medicine ER -