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Multicentre surveillance of prevalence of the 23S rRNA A2058G and A2059G point mutations and molecular subtypes of Treponema pallidum in Taiwan, 2009-2013.
Clin Microbiol Infect. 2014 Aug; 20(8):802-7.CM

Abstract

Resistance mutations A2058G and A2059G, within the 23S rRNA gene of Treponema pallidum, have been reported to cause treatment failures in patients receiving azithromycin for syphilis. Genotyping of T. pallidum strains sequentially isolated from patients with recurrent syphilis is rarely performed. From September 2009 to August 2013, we collected 658 clinical specimens from 375 patients who presented with syphilis for genotyping to examine the number of 60-bp repeats in the acidic repeat protein (arp) gene, T. pallidum repeat (tpr) polymorphism, and tp0548 gene, and to detect A2058G and A2059G point mutations by restriction fragment length polymorphism. Treponemal DNA was identified in 45.2% (n = 298) of the specimens that were collected from 216 (57.6%) patients; 268 (40.7%) specimens tested positive for the 23S rRNA gene, and were examined for macrolide resistance. Two isolates (0.7%) harboured the A2058G mutation, and no A2059G mutation was identified. A total of 14 strains of T. pallidum were identified, with 14f/f (57.5%) and 14b/c (10.0%) being the two predominant strains. Forty patients who presented with recurrent episodes of syphilis had T. pallidum DNA identified from the initial and subsequent episodes, with five cases showing strain discrepancies. One patient had two strains identified from different clinical specimens collected in the same episode. Our findings show that 14f/f is the most common T. pallidum strain in Taiwan, where the prevalence of T. pallidum strains that show A2058G or A2059G mutation remains low. Different genotypes of T. pallidum can be identified in patients with recurrent episodes of syphilis.

Authors+Show Affiliations

Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24438059

Citation

Wu, B-R, et al. "Multicentre Surveillance of Prevalence of the 23S rRNA A2058G and A2059G Point Mutations and Molecular Subtypes of Treponema Pallidum in Taiwan, 2009-2013." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 20, no. 8, 2014, pp. 802-7.
Wu BR, Yang CJ, Tsai MS, et al. Multicentre surveillance of prevalence of the 23S rRNA A2058G and A2059G point mutations and molecular subtypes of Treponema pallidum in Taiwan, 2009-2013. Clin Microbiol Infect. 2014;20(8):802-7.
Wu, B. R., Yang, C. J., Tsai, M. S., Lee, K. Y., Lee, N. Y., Huang, W. C., Wu, H., Lee, C. H., Chen, T. C., Ko, W. C., Lin, H. H., Lu, P. L., Chen, Y. H., Liu, W. C., Yang, S. P., Wu, P. Y., Su, Y. C., Hung, C. C., & Chang, S. Y. (2014). Multicentre surveillance of prevalence of the 23S rRNA A2058G and A2059G point mutations and molecular subtypes of Treponema pallidum in Taiwan, 2009-2013. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(8), 802-7. https://doi.org/10.1111/1469-0691.12529
Wu BR, et al. Multicentre Surveillance of Prevalence of the 23S rRNA A2058G and A2059G Point Mutations and Molecular Subtypes of Treponema Pallidum in Taiwan, 2009-2013. Clin Microbiol Infect. 2014;20(8):802-7. PubMed PMID: 24438059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multicentre surveillance of prevalence of the 23S rRNA A2058G and A2059G point mutations and molecular subtypes of Treponema pallidum in Taiwan, 2009-2013. AU - Wu,B-R, AU - Yang,C-J, AU - Tsai,M-S, AU - Lee,K-Y, AU - Lee,N-Y, AU - Huang,W-C, AU - Wu,H, AU - Lee,C-H, AU - Chen,T-C, AU - Ko,W-C, AU - Lin,H-H, AU - Lu,P-L, AU - Chen,Y-H, AU - Liu,W-C, AU - Yang,S-P, AU - Wu,P-Y, AU - Su,Y-C, AU - Hung,C-C, AU - Chang,S-Y, Y1 - 2014/02/13/ PY - 2013/10/01/received PY - 2014/01/10/revised PY - 2014/01/12/accepted PY - 2014/1/21/entrez PY - 2014/1/21/pubmed PY - 2015/4/23/medline KW - Antimicrobial resistance KW - azithromycin KW - genotyping KW - macrolides KW - molecular epidemiology KW - syphilis SP - 802 EP - 7 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 20 IS - 8 N2 - Resistance mutations A2058G and A2059G, within the 23S rRNA gene of Treponema pallidum, have been reported to cause treatment failures in patients receiving azithromycin for syphilis. Genotyping of T. pallidum strains sequentially isolated from patients with recurrent syphilis is rarely performed. From September 2009 to August 2013, we collected 658 clinical specimens from 375 patients who presented with syphilis for genotyping to examine the number of 60-bp repeats in the acidic repeat protein (arp) gene, T. pallidum repeat (tpr) polymorphism, and tp0548 gene, and to detect A2058G and A2059G point mutations by restriction fragment length polymorphism. Treponemal DNA was identified in 45.2% (n = 298) of the specimens that were collected from 216 (57.6%) patients; 268 (40.7%) specimens tested positive for the 23S rRNA gene, and were examined for macrolide resistance. Two isolates (0.7%) harboured the A2058G mutation, and no A2059G mutation was identified. A total of 14 strains of T. pallidum were identified, with 14f/f (57.5%) and 14b/c (10.0%) being the two predominant strains. Forty patients who presented with recurrent episodes of syphilis had T. pallidum DNA identified from the initial and subsequent episodes, with five cases showing strain discrepancies. One patient had two strains identified from different clinical specimens collected in the same episode. Our findings show that 14f/f is the most common T. pallidum strain in Taiwan, where the prevalence of T. pallidum strains that show A2058G or A2059G mutation remains low. Different genotypes of T. pallidum can be identified in patients with recurrent episodes of syphilis. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/24438059/Multicentre_surveillance_of_prevalence_of_the_23S_rRNA_A2058G_and_A2059G_point_mutations_and_molecular_subtypes_of_Treponema_pallidum_in_Taiwan_2009_2013_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)60377-3 DB - PRIME DP - Unbound Medicine ER -