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Anti-hyperglycemic activity of Centella asiatica is partly mediated by carbohydrase inhibition and glucose-fiber binding.

Abstract

BACKGROUND

Centella asiatica (C. asiatica) was previously reported to have anti-hyperglycemic effects in animal diabetic model rats. However, its activity on organ and tissue level remains unstudied. Our study aims at exploring the possible effects, C. asiatica extract and insoluble fiber has on carbohydrate absorption, insulin secretion, insulin sensitivity and glucose utilization.

METHODS

For primary evaluation of anti-hyperglycemic activity, we measured Fasting Blood Glucose and performed Glucose Tolerance Test, in type 2 diabetic rats. To further study the pancreatic effect and glucose utilization, plasma insulin concentration, insulin secreted from isolated rat islets and liver glycogen were assayed. Effect on carbohydrate break down was assayed using intestinal disaccharidase enzyme, α-amylase inhibition assays and Six-Segment study of the GI tract. Effect of C. asiatica on glucose absorption was studied by an in-situ, perfused, intestinal model in rats and by glucose-fiber binding assay. Gastrointestinal motility was seen by a BaSO4 milk traverse test. Additionally, a complete lipid profile assay, after a chronic study, was conducted.

RESULTS

C. asiatica showed no significant change in insulin secretion in-vivo and in isolated rat islets. Additionally, no effect of the extract was seen on liver glycogen deposition. Retarded glucose absorption was seen in the in-situ perfused rat intestinal model at a dose. The extract was also found to inhibit action of both intestinal disaccharidase and α-amylase. This was confirmed, yet again, via the Six Segment study, where sucrose digestion was found to be inhibited throughout the length of the GI Tract. Significant glucose-fiber binding was demonstrated in the in-vitro models. During the chronic study, body mass of C. asiatica treated Type 2 diabetic rats returned to normal and their polydipsic and polyphagic conditions were also improved. Chronic treatment of C. asiatica also improved subject's lipid profile.

CONCLUSION

A combination of in-vitro, in-vivo and in-situ tests confirmed the anti-hyperglycemic activity of C. asiatica and its tissue level mechanism. Further study is required to fully elucidate the effect this extract or the active compounds have on the individual glucose transporters and the precise mechanism of glucose-fiber binding.

Authors+Show Affiliations

Department of Pharmacy, North South University, Dhaka, Bangladesh. ashraful_kabir@ymail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24438380

Citation

Kabir, Ashraf Ul, et al. "Anti-hyperglycemic Activity of Centella Asiatica Is Partly Mediated By Carbohydrase Inhibition and Glucose-fiber Binding." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 31.
Kabir AU, Samad MB, D'Costa NM, et al. Anti-hyperglycemic activity of Centella asiatica is partly mediated by carbohydrase inhibition and glucose-fiber binding. BMC Complement Altern Med. 2014;14:31.
Kabir, A. U., Samad, M. B., D'Costa, N. M., Akhter, F., Ahmed, A., & Hannan, J. M. (2014). Anti-hyperglycemic activity of Centella asiatica is partly mediated by carbohydrase inhibition and glucose-fiber binding. BMC Complementary and Alternative Medicine, 14, p. 31. doi:10.1186/1472-6882-14-31.
Kabir AU, et al. Anti-hyperglycemic Activity of Centella Asiatica Is Partly Mediated By Carbohydrase Inhibition and Glucose-fiber Binding. BMC Complement Altern Med. 2014 Jan 18;14:31. PubMed PMID: 24438380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-hyperglycemic activity of Centella asiatica is partly mediated by carbohydrase inhibition and glucose-fiber binding. AU - Kabir,Ashraf Ul, AU - Samad,Mehdi Bin, AU - D'Costa,Ninadh Malrina, AU - Akhter,Farjana, AU - Ahmed,Arif, AU - Hannan,J M A, Y1 - 2014/01/18/ PY - 2013/08/10/received PY - 2014/01/15/accepted PY - 2014/1/21/entrez PY - 2014/1/21/pubmed PY - 2015/10/9/medline SP - 31 EP - 31 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Centella asiatica (C. asiatica) was previously reported to have anti-hyperglycemic effects in animal diabetic model rats. However, its activity on organ and tissue level remains unstudied. Our study aims at exploring the possible effects, C. asiatica extract and insoluble fiber has on carbohydrate absorption, insulin secretion, insulin sensitivity and glucose utilization. METHODS: For primary evaluation of anti-hyperglycemic activity, we measured Fasting Blood Glucose and performed Glucose Tolerance Test, in type 2 diabetic rats. To further study the pancreatic effect and glucose utilization, plasma insulin concentration, insulin secreted from isolated rat islets and liver glycogen were assayed. Effect on carbohydrate break down was assayed using intestinal disaccharidase enzyme, α-amylase inhibition assays and Six-Segment study of the GI tract. Effect of C. asiatica on glucose absorption was studied by an in-situ, perfused, intestinal model in rats and by glucose-fiber binding assay. Gastrointestinal motility was seen by a BaSO4 milk traverse test. Additionally, a complete lipid profile assay, after a chronic study, was conducted. RESULTS: C. asiatica showed no significant change in insulin secretion in-vivo and in isolated rat islets. Additionally, no effect of the extract was seen on liver glycogen deposition. Retarded glucose absorption was seen in the in-situ perfused rat intestinal model at a dose. The extract was also found to inhibit action of both intestinal disaccharidase and α-amylase. This was confirmed, yet again, via the Six Segment study, where sucrose digestion was found to be inhibited throughout the length of the GI Tract. Significant glucose-fiber binding was demonstrated in the in-vitro models. During the chronic study, body mass of C. asiatica treated Type 2 diabetic rats returned to normal and their polydipsic and polyphagic conditions were also improved. Chronic treatment of C. asiatica also improved subject's lipid profile. CONCLUSION: A combination of in-vitro, in-vivo and in-situ tests confirmed the anti-hyperglycemic activity of C. asiatica and its tissue level mechanism. Further study is required to fully elucidate the effect this extract or the active compounds have on the individual glucose transporters and the precise mechanism of glucose-fiber binding. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24438380/Anti_hyperglycemic_activity_of_Centella_asiatica_is_partly_mediated_by_carbohydrase_inhibition_and_glucose_fiber_binding_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-31 DB - PRIME DP - Unbound Medicine ER -