Tags

Type your tag names separated by a space and hit enter

Involvement of the blood-brain barrier opening in cognitive decline in aged rats following orthopedic surgery and high concentration of sevoflurane inhalation.
Brain Res 2014; 1551:13-24BR

Abstract

The underlying causes of postoperative cognitive decline (POCD) in old patients remained unelucidated, and there are little descriptions on mechanisms associated with the blood-brain barrier (BBB) disruption during POCD. We therefore tested the effects of orthopedic surgery with different concentrations of sevoflurane for 2 h on the behavior test and the BBB permeability in aged rats. 18-month rats were divided into control group and surgical group with propofol anesthesia (0.7 mgkg(-1) min(-1)) and 1.0 MAC, 1.3 MAC, and 1.5 MAC sevoflurane inhalation for 2 h. We assessed their cognitive function via Y-maze and fear conditioning test on day 1, 3, and 7 after experiments. Animals were then assigned to control group, propofol (2 h, 0.7 mgkg(-1) min(-1)) group, surgery plus propofol group and surgery plus 1.5 MAC sevoflurane inhalation for 2h. Their hippocampal BBB permeability was detected with Evans blue quantification. Alterations of tight junctions in hippocampus were measured with occludin and claudin-5 western blot. Then we assessed matrix metalloproteinase-2,9 (MMP-2,9) via western blot and immunohistochemistry staining at day 1, 3, 7, and 14 after experiments. Surgery impaired cognitive function and increased Evans blue leakage into the hippocampus in aged rats while 2 h of 1.5 MAC sevoflurane inhalation potentiated these effects. Surgery induced occludin protein expression decreases and MMP-2,9 proteins increase and these influences can be enhanced by high concentration of sevoflurane inhalation. In conclusion, 1.5 MAC sevoflurane for 2 h exacerbated cognitive impairment induced by orthopedic surgery in aged rats and the breach in BBB may be involved in this process.

Authors+Show Affiliations

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: hunan1987222@sina.com.Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, China. Electronic address: gdy1234@hotmail.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: wanghy819@hotmail.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: mzk2011@126.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: doctorwangchao@Gmail.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: liyizelisa@126.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: 0208wcy@163.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: wangchenxu2836@sina.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: yuyonghao@126.com.Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, China. Electronic address: wang_guolin@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24440777

Citation

Hu, Nan, et al. "Involvement of the Blood-brain Barrier Opening in Cognitive Decline in Aged Rats Following Orthopedic Surgery and High Concentration of Sevoflurane Inhalation." Brain Research, vol. 1551, 2014, pp. 13-24.
Hu N, Guo D, Wang H, et al. Involvement of the blood-brain barrier opening in cognitive decline in aged rats following orthopedic surgery and high concentration of sevoflurane inhalation. Brain Res. 2014;1551:13-24.
Hu, N., Guo, D., Wang, H., Xie, K., Wang, C., Li, Y., ... Wang, G. (2014). Involvement of the blood-brain barrier opening in cognitive decline in aged rats following orthopedic surgery and high concentration of sevoflurane inhalation. Brain Research, 1551, pp. 13-24. doi:10.1016/j.brainres.2014.01.015.
Hu N, et al. Involvement of the Blood-brain Barrier Opening in Cognitive Decline in Aged Rats Following Orthopedic Surgery and High Concentration of Sevoflurane Inhalation. Brain Res. 2014 Mar 10;1551:13-24. PubMed PMID: 24440777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of the blood-brain barrier opening in cognitive decline in aged rats following orthopedic surgery and high concentration of sevoflurane inhalation. AU - Hu,Nan, AU - Guo,Dongyong, AU - Wang,Haiyun, AU - Xie,Keliang, AU - Wang,Chao, AU - Li,Yize, AU - Wang,Chunyan, AU - Wang,Chenxu, AU - Yu,Yonghao, AU - Wang,Guolin, Y1 - 2014/01/16/ PY - 2013/11/11/received PY - 2014/01/09/revised PY - 2014/01/14/accepted PY - 2014/1/21/entrez PY - 2014/1/21/pubmed PY - 2014/10/16/medline KW - Blood–brain barrier KW - Matrix metalloproteinase-2,9 KW - Orthopedic surgery KW - Sevoflurane KW - Tight junction SP - 13 EP - 24 JF - Brain research JO - Brain Res. VL - 1551 N2 - The underlying causes of postoperative cognitive decline (POCD) in old patients remained unelucidated, and there are little descriptions on mechanisms associated with the blood-brain barrier (BBB) disruption during POCD. We therefore tested the effects of orthopedic surgery with different concentrations of sevoflurane for 2 h on the behavior test and the BBB permeability in aged rats. 18-month rats were divided into control group and surgical group with propofol anesthesia (0.7 mgkg(-1) min(-1)) and 1.0 MAC, 1.3 MAC, and 1.5 MAC sevoflurane inhalation for 2 h. We assessed their cognitive function via Y-maze and fear conditioning test on day 1, 3, and 7 after experiments. Animals were then assigned to control group, propofol (2 h, 0.7 mgkg(-1) min(-1)) group, surgery plus propofol group and surgery plus 1.5 MAC sevoflurane inhalation for 2h. Their hippocampal BBB permeability was detected with Evans blue quantification. Alterations of tight junctions in hippocampus were measured with occludin and claudin-5 western blot. Then we assessed matrix metalloproteinase-2,9 (MMP-2,9) via western blot and immunohistochemistry staining at day 1, 3, 7, and 14 after experiments. Surgery impaired cognitive function and increased Evans blue leakage into the hippocampus in aged rats while 2 h of 1.5 MAC sevoflurane inhalation potentiated these effects. Surgery induced occludin protein expression decreases and MMP-2,9 proteins increase and these influences can be enhanced by high concentration of sevoflurane inhalation. In conclusion, 1.5 MAC sevoflurane for 2 h exacerbated cognitive impairment induced by orthopedic surgery in aged rats and the breach in BBB may be involved in this process. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/24440777/Involvement_of_the_blood_brain_barrier_opening_in_cognitive_decline_in_aged_rats_following_orthopedic_surgery_and_high_concentration_of_sevoflurane_inhalation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(14)00068-7 DB - PRIME DP - Unbound Medicine ER -