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The influence of carbon monoxide bolus on the measurement of total haemoglobin mass using the optimized CO-rebreathing method.
Physiol Meas. 2014 Feb; 35(2):N11-9.PM

Abstract

The optimized carbon monoxide (CO) rebreathing method (oCOR-method) is routinely used to measure total haemoglobin mass (tHbmass). The tHbmass measure is subject to a test-retest typical error of ~2%, mostly from the precision of carboxyhaemoglobin (HbCO) measurement. We hypothesized that tHbmass would be robust to differences in the bolus of CO administered during the oCOR-method. Twelve participants (ten males and two females; age 27 ± 6 yr, height 177 ± 11 cm and mass 73.9 ± 12.1 kg) completed the oCOR-method on four occasions. Different bolus of CO were administered (LOW: 0.6 ml kg(-1); MED1: 1.0 ml kg(-1) and HIGH: 1.4 ml kg(-1)); to determine the reliability of MED1, a second trial was conducted (MED2). tHbmass was found to be significantly less from the HIGH CO bolus (776 ± 148 g) when compared to the LOW CO (791 ± 149 g) or MED1 CO (788 ± 149 g) trials. MED2 CO was 785 ± 150 g. The measurement of tHbmass is repeatable to within 0.8%, but a small and notable difference was seen when using a HIGH CO bolus (1.4 to 1.9% less), potentially due to differences in CO uptake kinetics. Previously, an improved precision of the oCOR-method was thought to require a higher bolus of CO (i.e. larger Δ%HbCO), as commercial hemoximeters only estimate %HbCO levels to a single decimal place (usually ± 0.1%). With the new hemoximeter used in this study, a bolus of 1.0 ml kg(-1) allows adequate precision with acceptable safety.

Authors

Turner G
No affiliation info available
Pringle JS
No affiliation info available
Ingham SA
No affiliation info available
Fudge BW
No affiliation info available
Richardson AJ
No affiliation info available
Maxwell NS
No affiliation info available

MeSH

AdultArtifactsBlood Chemical AnalysisCarbon MonoxideDose-Response Relationship, DrugFemaleHemoglobinsHumansMaleRespiration

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

24451475

Citation

Turner, G, et al. "The Influence of Carbon Monoxide Bolus On the Measurement of Total Haemoglobin Mass Using the Optimized CO-rebreathing Method." Physiological Measurement, vol. 35, no. 2, 2014, pp. N11-9.
Turner G, Pringle JS, Ingham SA, et al. The influence of carbon monoxide bolus on the measurement of total haemoglobin mass using the optimized CO-rebreathing method. Physiol Meas. 2014;35(2):N11-9.
Turner, G., Pringle, J. S., Ingham, S. A., Fudge, B. W., Richardson, A. J., & Maxwell, N. S. (2014). The influence of carbon monoxide bolus on the measurement of total haemoglobin mass using the optimized CO-rebreathing method. Physiological Measurement, 35(2), N11-9.
Turner G, et al. The Influence of Carbon Monoxide Bolus On the Measurement of Total Haemoglobin Mass Using the Optimized CO-rebreathing Method. Physiol Meas. 2014;35(2):N11-9. PubMed PMID: 24451475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of carbon monoxide bolus on the measurement of total haemoglobin mass using the optimized CO-rebreathing method. AU - Turner,G, AU - Pringle,J S M, AU - Ingham,S A, AU - Fudge,B W, AU - Richardson,A J, AU - Maxwell,N S, PY - 2014/1/24/entrez PY - 2014/1/24/pubmed PY - 2014/10/1/medline SP - N11 EP - 9 JF - Physiological measurement JO - Physiol Meas VL - 35 IS - 2 N2 - The optimized carbon monoxide (CO) rebreathing method (oCOR-method) is routinely used to measure total haemoglobin mass (tHbmass). The tHbmass measure is subject to a test-retest typical error of ~2%, mostly from the precision of carboxyhaemoglobin (HbCO) measurement. We hypothesized that tHbmass would be robust to differences in the bolus of CO administered during the oCOR-method. Twelve participants (ten males and two females; age 27 ± 6 yr, height 177 ± 11 cm and mass 73.9 ± 12.1 kg) completed the oCOR-method on four occasions. Different bolus of CO were administered (LOW: 0.6 ml kg(-1); MED1: 1.0 ml kg(-1) and HIGH: 1.4 ml kg(-1)); to determine the reliability of MED1, a second trial was conducted (MED2). tHbmass was found to be significantly less from the HIGH CO bolus (776 ± 148 g) when compared to the LOW CO (791 ± 149 g) or MED1 CO (788 ± 149 g) trials. MED2 CO was 785 ± 150 g. The measurement of tHbmass is repeatable to within 0.8%, but a small and notable difference was seen when using a HIGH CO bolus (1.4 to 1.9% less), potentially due to differences in CO uptake kinetics. Previously, an improved precision of the oCOR-method was thought to require a higher bolus of CO (i.e. larger Δ%HbCO), as commercial hemoximeters only estimate %HbCO levels to a single decimal place (usually ± 0.1%). With the new hemoximeter used in this study, a bolus of 1.0 ml kg(-1) allows adequate precision with acceptable safety. SN - 1361-6579 UR - https://www.unboundmedicine.com/medline/citation/24451475/The_influence_of_carbon_monoxide_bolus_on_the_measurement_of_total_haemoglobin_mass_using_the_optimized_CO_rebreathing_method_ DB - PRIME DP - Unbound Medicine ER -