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Extract of Aronia melanocarpa-modified hemostasis: in vitro studies.
Eur J Nutr. 2014 Oct; 53(7):1493-502.EJ

Abstract

PURPOSE

Aronia melanocarpa has an extremely high content of procyanidins and anthocyanins. The multidirectional benefits of consumption of these berries are widely reported. Although numerous studies confirmed the influence of polyphenols on various stages of hemostasis, the exact mechanism of this phenomenon is not understood. The aim of our study was to evaluate the in vitro effect of A. melanocarpa extract on various parameters of hemostasis.

METHODS

Adenosine 5'-diphosphate (ADP)-induced aggregation was measured with turbidimetric method. Spontaneous and ADP-activated platelet adhesion were investigated using a colorimetric method. The global assay of coagulation and fibrinolysis was performed with the use of optical clotting and lysis (CL) test. Thrombin (0.5 IU/mL) and tissue plasminogen activator (60 ng/mL) were used to obtain a CL curve. The activity of thrombin and plasmin was determined by means of chromogenic substrate (S-2238, S-2251) RESULTS: The aronia extract contributed to the reduction in spontaneous and ADP-activated platelet adhesion. A significant increase in overall potential of CL as well as significant changes in key parameters of these processes (T t-thrombin time, F vo-initial plasma clotting velocity, and L max-maximum lysis) was reported. Chokeberry extract significantly inhibited the amidolytic activity of thrombin and plasmin.

CONCLUSION

Our in vitro findings indicate a complex mechanism of influence of chokeberry polyphenols on platelet activity and the overall potential of CL. We confirmed that chokeberry inhibits the amidolytic activity of thrombin. It was demonstrated for the first time that chokeberry polyphenols inhibit the amidolytic activity of another serine protease, i.e., plasmin, which is the main fibrinolytic enzyme. Furthermore, our research points out a significant contribution of other plasma components and fibrinogen in the modulation of hemostasis by polyphenols.

Links

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Authors+Show Affiliations

Sikora J
Department of Pharmaceutical Chemistry and Drug Analyses, Medical University of Lodz, Muszynskiego 1, 90-151, Lodz, Poland, joanna.sikora@umed.lodz.pl.
Markowicz-Piasecka M
No affiliation info available
Broncel M
No affiliation info available
Mikiciuk-Olasik E
No affiliation info available

MeSH

AnthocyaninsBlood CoagulationBlood PlateletsCell AdhesionFibrinolysisFruitHemostasisHumansPhotiniaPlant ExtractsPolyphenolsProanthocyanidinsThrombinTissue Plasminogen Activator

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24452534

Citation

Sikora, Joanna, et al. "Extract of Aronia Melanocarpa-modified Hemostasis: in Vitro Studies." European Journal of Nutrition, vol. 53, no. 7, 2014, pp. 1493-502.
Sikora J, Markowicz-Piasecka M, Broncel M, et al. Extract of Aronia melanocarpa-modified hemostasis: in vitro studies. Eur J Nutr. 2014;53(7):1493-502.
Sikora, J., Markowicz-Piasecka, M., Broncel, M., & Mikiciuk-Olasik, E. (2014). Extract of Aronia melanocarpa-modified hemostasis: in vitro studies. European Journal of Nutrition, 53(7), 1493-502. https://doi.org/10.1007/s00394-014-0653-8
Sikora J, et al. Extract of Aronia Melanocarpa-modified Hemostasis: in Vitro Studies. Eur J Nutr. 2014;53(7):1493-502. PubMed PMID: 24452534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extract of Aronia melanocarpa-modified hemostasis: in vitro studies. AU - Sikora,Joanna, AU - Markowicz-Piasecka,Magdalena, AU - Broncel,Marlena, AU - Mikiciuk-Olasik,Elżbieta, Y1 - 2014/01/23/ PY - 2013/10/18/received PY - 2014/01/12/accepted PY - 2014/1/24/entrez PY - 2014/1/24/pubmed PY - 2015/6/17/medline SP - 1493 EP - 502 JF - European journal of nutrition JO - Eur J Nutr VL - 53 IS - 7 N2 - PURPOSE: Aronia melanocarpa has an extremely high content of procyanidins and anthocyanins. The multidirectional benefits of consumption of these berries are widely reported. Although numerous studies confirmed the influence of polyphenols on various stages of hemostasis, the exact mechanism of this phenomenon is not understood. The aim of our study was to evaluate the in vitro effect of A. melanocarpa extract on various parameters of hemostasis. METHODS: Adenosine 5'-diphosphate (ADP)-induced aggregation was measured with turbidimetric method. Spontaneous and ADP-activated platelet adhesion were investigated using a colorimetric method. The global assay of coagulation and fibrinolysis was performed with the use of optical clotting and lysis (CL) test. Thrombin (0.5 IU/mL) and tissue plasminogen activator (60 ng/mL) were used to obtain a CL curve. The activity of thrombin and plasmin was determined by means of chromogenic substrate (S-2238, S-2251) RESULTS: The aronia extract contributed to the reduction in spontaneous and ADP-activated platelet adhesion. A significant increase in overall potential of CL as well as significant changes in key parameters of these processes (T t-thrombin time, F vo-initial plasma clotting velocity, and L max-maximum lysis) was reported. Chokeberry extract significantly inhibited the amidolytic activity of thrombin and plasmin. CONCLUSION: Our in vitro findings indicate a complex mechanism of influence of chokeberry polyphenols on platelet activity and the overall potential of CL. We confirmed that chokeberry inhibits the amidolytic activity of thrombin. It was demonstrated for the first time that chokeberry polyphenols inhibit the amidolytic activity of another serine protease, i.e., plasmin, which is the main fibrinolytic enzyme. Furthermore, our research points out a significant contribution of other plasma components and fibrinogen in the modulation of hemostasis by polyphenols. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/24452534/Extract_of_Aronia_melanocarpa_modified_hemostasis:_in_vitro_studies_ DB - PRIME DP - Unbound Medicine ER -