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Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum).
J Nat Prod 2014; 77(2):193-9JN

Abstract

Silybin A (1), silybin B (2), and isosilybin A (3), three of the seven flavonolignans that constitute silymarin, an extract of the fruits of milk thistle (Silybum marianum), were detected for the first time from a fungal endophyte, Aspergillus iizukae, isolated from the surface-sterilized leaves of S. marianum. The flavonolignans were identified using a UPLC-PDA-HRMS-MS/MS method by matching retention times, HRMS, and MS/MS data with authentic reference compounds. Attenuation of flavonolignan production was observed following successive subculturing of the original flavonolignan-producing culture, as is often the case with endophytes that produce plant-based secondary metabolites. However, production of 1 and 2 resumed when attenuated spores were harvested from cultures grown on a medium to which autoclaved leaves of S. marianum were added. The cycle of attenuation followed by resumed biosynthesis of these flavonolignans was replicated in triplicate.

Authors+Show Affiliations

Department of Chemistry and Biochemistry, University of North Carolina at Greensboro , Greensboro, North Carolina 27402, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24456525

Citation

El-Elimat, Tamam, et al. "Flavonolignans From Aspergillus Iizukae, a Fungal Endophyte of Milk Thistle (Silybum Marianum)." Journal of Natural Products, vol. 77, no. 2, 2014, pp. 193-9.
El-Elimat T, Raja HA, Graf TN, et al. Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum). J Nat Prod. 2014;77(2):193-9.
El-Elimat, T., Raja, H. A., Graf, T. N., Faeth, S. H., Cech, N. B., & Oberlies, N. H. (2014). Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum). Journal of Natural Products, 77(2), pp. 193-9. doi:10.1021/np400955q.
El-Elimat T, et al. Flavonolignans From Aspergillus Iizukae, a Fungal Endophyte of Milk Thistle (Silybum Marianum). J Nat Prod. 2014 Feb 28;77(2):193-9. PubMed PMID: 24456525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonolignans from Aspergillus iizukae, a fungal endophyte of milk thistle (Silybum marianum). AU - El-Elimat,Tamam, AU - Raja,Huzefa A, AU - Graf,Tyler N, AU - Faeth,Stanley H, AU - Cech,Nadja B, AU - Oberlies,Nicholas H, Y1 - 2014/01/23/ PY - 2014/1/25/entrez PY - 2014/1/25/pubmed PY - 2014/8/8/medline SP - 193 EP - 9 JF - Journal of natural products JO - J. Nat. Prod. VL - 77 IS - 2 N2 - Silybin A (1), silybin B (2), and isosilybin A (3), three of the seven flavonolignans that constitute silymarin, an extract of the fruits of milk thistle (Silybum marianum), were detected for the first time from a fungal endophyte, Aspergillus iizukae, isolated from the surface-sterilized leaves of S. marianum. The flavonolignans were identified using a UPLC-PDA-HRMS-MS/MS method by matching retention times, HRMS, and MS/MS data with authentic reference compounds. Attenuation of flavonolignan production was observed following successive subculturing of the original flavonolignan-producing culture, as is often the case with endophytes that produce plant-based secondary metabolites. However, production of 1 and 2 resumed when attenuated spores were harvested from cultures grown on a medium to which autoclaved leaves of S. marianum were added. The cycle of attenuation followed by resumed biosynthesis of these flavonolignans was replicated in triplicate. SN - 1520-6025 UR - https://www.unboundmedicine.com/medline/citation/24456525/Flavonolignans_from_Aspergillus_iizukae_a_fungal_endophyte_of_milk_thistle__Silybum_marianum__ L2 - https://dx.doi.org/10.1021/np400955q DB - PRIME DP - Unbound Medicine ER -