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Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats.
Phytomedicine. 2014 Apr 15; 21(5):624-30.P

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Cucumis prophetarum (L.) is used in traditional Indian medicine for the treatment of inflammation related problems.

AIM OF THE STUDY

The present investigation was designed to study the effect of N-Trisaccharide (a new compound isolated from the fruit of C. prophetarum (L.)) on hyperglycemia in streptozotocin (STZ)-nicotinamide (NA) induced type 2 diabetic rats.

MATERIALS AND METHODS

Different doses of N-Trisaccharide (25 and 50 mg/kgb.w.) were administered once daily for 28 days to STZ-NA induced diabetic rats. Plasma insulin and glycogen levels were measured. The activities of hexokinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and glycogen phosphorylase were measured. Further, histological studies on pancreas were also carried out.

RESULTS

The active compound at doses of 25 and 50 mg/kgb.w. given orally for 14 days showed 47.7% and 69.3% antihyperglycemic activity, respectively. Treatment at the same doses for 28 days provided complete protection against STZ-NA challenge (65 and 230 mg/kgb.w., respectively), intraperitoneally. N-Trisaccharide significantly (p≤0.05) increased the plasma insulin and liver glycogen levels in diabetic rats. The altered enzyme activities of carbohydrate metabolism in the liver and kidney of the diabetic rats were significantly (p≤0.05) improved. Additionally, N-Trisaccharide increased glycogen synthase and decreased glycogen phosphorylase activity in diabetic rats. Histological studies confirmed an increase in insulin level is due to stimulation of injured pancreatic β-cells.

CONCLUSION

The results of the study suggested that N-Trisaccharide possesses propitious effect on STZ-NA induced type 2 diabetes, indicating its usefulness in diabetes management.

Authors+Show Affiliations

DOS in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India; DOS in Biochemistry, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India.DOS in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India. Electronic address: nlakshmidevi7@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24462215

Citation

Kavishankar, G B., and N Lakshmidevi. "Anti-diabetic Effect of a Novel N-Trisaccharide Isolated From Cucumis Prophetarum On Streptozotocin-nicotinamide Induced Type 2 Diabetic Rats." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 21, no. 5, 2014, pp. 624-30.
Kavishankar GB, Lakshmidevi N. Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats. Phytomedicine. 2014;21(5):624-30.
Kavishankar, G. B., & Lakshmidevi, N. (2014). Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 21(5), 624-30. https://doi.org/10.1016/j.phymed.2013.12.002
Kavishankar GB, Lakshmidevi N. Anti-diabetic Effect of a Novel N-Trisaccharide Isolated From Cucumis Prophetarum On Streptozotocin-nicotinamide Induced Type 2 Diabetic Rats. Phytomedicine. 2014 Apr 15;21(5):624-30. PubMed PMID: 24462215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats. AU - Kavishankar,G B, AU - Lakshmidevi,N, Y1 - 2014/01/22/ PY - 2013/01/29/received PY - 2013/10/29/revised PY - 2013/12/20/accepted PY - 2014/1/28/entrez PY - 2014/1/28/pubmed PY - 2014/12/17/medline KW - Antidiabetic activity KW - Cucumis prophetarum KW - Glucose metabolism KW - N-Trisaccharide KW - Streptozotocin–nicotinamide KW - Type 2 diabetes SP - 624 EP - 30 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 21 IS - 5 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Cucumis prophetarum (L.) is used in traditional Indian medicine for the treatment of inflammation related problems. AIM OF THE STUDY: The present investigation was designed to study the effect of N-Trisaccharide (a new compound isolated from the fruit of C. prophetarum (L.)) on hyperglycemia in streptozotocin (STZ)-nicotinamide (NA) induced type 2 diabetic rats. MATERIALS AND METHODS: Different doses of N-Trisaccharide (25 and 50 mg/kgb.w.) were administered once daily for 28 days to STZ-NA induced diabetic rats. Plasma insulin and glycogen levels were measured. The activities of hexokinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and glycogen phosphorylase were measured. Further, histological studies on pancreas were also carried out. RESULTS: The active compound at doses of 25 and 50 mg/kgb.w. given orally for 14 days showed 47.7% and 69.3% antihyperglycemic activity, respectively. Treatment at the same doses for 28 days provided complete protection against STZ-NA challenge (65 and 230 mg/kgb.w., respectively), intraperitoneally. N-Trisaccharide significantly (p≤0.05) increased the plasma insulin and liver glycogen levels in diabetic rats. The altered enzyme activities of carbohydrate metabolism in the liver and kidney of the diabetic rats were significantly (p≤0.05) improved. Additionally, N-Trisaccharide increased glycogen synthase and decreased glycogen phosphorylase activity in diabetic rats. Histological studies confirmed an increase in insulin level is due to stimulation of injured pancreatic β-cells. CONCLUSION: The results of the study suggested that N-Trisaccharide possesses propitious effect on STZ-NA induced type 2 diabetes, indicating its usefulness in diabetes management. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/24462215/Anti_diabetic_effect_of_a_novel_N_Trisaccharide_isolated_from_Cucumis_prophetarum_on_streptozotocin_nicotinamide_induced_type_2_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(13)00477-7 DB - PRIME DP - Unbound Medicine ER -