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Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process.
Biochem Biophys Res Commun. 2014 Feb 07; 444(2):260-3.BB

Abstract

Superoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1Δ and sod2Δ strains. However, ccs1Δ mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1Δ, sod2Δ, and ccs1Δ mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process.

Authors+Show Affiliations

Izmir Institute of Technology, Department of Molecular Biology & Genetics, 35430 Urla, Izmir, Turkey.Izmir Institute of Technology, Department of Molecular Biology & Genetics, 35430 Urla, Izmir, Turkey.Izmir Institute of Technology, Department of Molecular Biology & Genetics, 35430 Urla, Izmir, Turkey. Electronic address: ahmetkoc@iyte.edu.tr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24462872

Citation

Muid, Khandaker Ashfaqul, et al. "Absence of Superoxide Dismutase Activity Causes Nuclear DNA Fragmentation During the Aging Process." Biochemical and Biophysical Research Communications, vol. 444, no. 2, 2014, pp. 260-3.
Muid KA, Karakaya HÇ, Koc A. Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process. Biochem Biophys Res Commun. 2014;444(2):260-3.
Muid, K. A., Karakaya, H. Ç., & Koc, A. (2014). Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process. Biochemical and Biophysical Research Communications, 444(2), 260-3. https://doi.org/10.1016/j.bbrc.2014.01.056
Muid KA, Karakaya HÇ, Koc A. Absence of Superoxide Dismutase Activity Causes Nuclear DNA Fragmentation During the Aging Process. Biochem Biophys Res Commun. 2014 Feb 7;444(2):260-3. PubMed PMID: 24462872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process. AU - Muid,Khandaker Ashfaqul, AU - Karakaya,Hüseyin Çaglar, AU - Koc,Ahmet, Y1 - 2014/01/22/ PY - 2014/01/08/received PY - 2014/01/15/accepted PY - 2014/1/28/entrez PY - 2014/1/28/pubmed PY - 2014/5/3/medline KW - Aging KW - Antioxidant KW - Comet assay KW - DNA damage KW - Longevity KW - Oxidative stress KW - ROS KW - Reactive oxygen species KW - SOD KW - Superoxide dismutase SP - 260 EP - 3 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 444 IS - 2 N2 - Superoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1Δ and sod2Δ strains. However, ccs1Δ mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1Δ, sod2Δ, and ccs1Δ mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/24462872/Absence_of_superoxide_dismutase_activity_causes_nuclear_DNA_fragmentation_during_the_aging_process_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(14)00080-1 DB - PRIME DP - Unbound Medicine ER -