Tags

Type your tag names separated by a space and hit enter

Decreased astroglial monocarboxylate transporter 4 expression in temporal lobe epilepsy.
Mol Neurobiol 2014; 50(2):327-38MN

Abstract

Efflux of monocaroxylates like lactate, pyruvate, and ketone bodies from astrocytes through monocarboxylate transporter 4 (MCT4) supplies the local neuron population with metabolic intermediates to meet energy requirements under conditions of increased demand. Disruption of this astroglial-neuron metabolic coupling pathway may contribute to epileptogenesis. We measured MCT4 expression in temporal lobe epileptic foci excised from patients with intractable epilepsy and in rats injected with pilocarpine, an animal model of temporal lobe epilepsy (TLE). Cortical MCT4 expression levels were significantly lower in TLE patients compared with controls, due at least partially to MCT4 promoter methylation. Expression of MCT4 also decreased progressively in pilocarpine-treated rats from 12 h to 14 days post-administration. Underexpression of MCT4 in cultured astrocytes induced by a short hairpin RNA promoted apoptosis. Knockdown of astrocyte MCT4 also suppressed excitatory amino acid transporter 1 (EAAT1) expression. Reduced MCT4 and EAAT1 expression by astrocytes may lead to neuronal hyperexcitability and epileptogenesis in the temporal lobe by reducing the supply of metabolic intermediates and by allowing accumulation of extracellular glutamate.

Authors+Show Affiliations

Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24464262

Citation

Liu, Bei, et al. "Decreased Astroglial Monocarboxylate Transporter 4 Expression in Temporal Lobe Epilepsy." Molecular Neurobiology, vol. 50, no. 2, 2014, pp. 327-38.
Liu B, Niu L, Shen MZ, et al. Decreased astroglial monocarboxylate transporter 4 expression in temporal lobe epilepsy. Mol Neurobiol. 2014;50(2):327-38.
Liu, B., Niu, L., Shen, M. Z., Gao, L., Wang, C., Li, J., ... Zhang, H. (2014). Decreased astroglial monocarboxylate transporter 4 expression in temporal lobe epilepsy. Molecular Neurobiology, 50(2), pp. 327-38. doi:10.1007/s12035-013-8619-z.
Liu B, et al. Decreased Astroglial Monocarboxylate Transporter 4 Expression in Temporal Lobe Epilepsy. Mol Neurobiol. 2014;50(2):327-38. PubMed PMID: 24464262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decreased astroglial monocarboxylate transporter 4 expression in temporal lobe epilepsy. AU - Liu,Bei, AU - Niu,Le, AU - Shen,Ming-Zhi, AU - Gao,Lei, AU - Wang,Chao, AU - Li,Jie, AU - Song,Li-Jia, AU - Tao,Ye, AU - Meng,Qiang, AU - Yang,Qian-Li, AU - Gao,Guo-Dong, AU - Zhang,Hua, Y1 - 2014/01/25/ PY - 2013/10/15/received PY - 2013/12/12/accepted PY - 2014/1/28/entrez PY - 2014/1/28/pubmed PY - 2015/9/12/medline SP - 327 EP - 38 JF - Molecular neurobiology JO - Mol. Neurobiol. VL - 50 IS - 2 N2 - Efflux of monocaroxylates like lactate, pyruvate, and ketone bodies from astrocytes through monocarboxylate transporter 4 (MCT4) supplies the local neuron population with metabolic intermediates to meet energy requirements under conditions of increased demand. Disruption of this astroglial-neuron metabolic coupling pathway may contribute to epileptogenesis. We measured MCT4 expression in temporal lobe epileptic foci excised from patients with intractable epilepsy and in rats injected with pilocarpine, an animal model of temporal lobe epilepsy (TLE). Cortical MCT4 expression levels were significantly lower in TLE patients compared with controls, due at least partially to MCT4 promoter methylation. Expression of MCT4 also decreased progressively in pilocarpine-treated rats from 12 h to 14 days post-administration. Underexpression of MCT4 in cultured astrocytes induced by a short hairpin RNA promoted apoptosis. Knockdown of astrocyte MCT4 also suppressed excitatory amino acid transporter 1 (EAAT1) expression. Reduced MCT4 and EAAT1 expression by astrocytes may lead to neuronal hyperexcitability and epileptogenesis in the temporal lobe by reducing the supply of metabolic intermediates and by allowing accumulation of extracellular glutamate. SN - 1559-1182 UR - https://www.unboundmedicine.com/medline/citation/24464262/Decreased_astroglial_monocarboxylate_transporter_4_expression_in_temporal_lobe_epilepsy_ L2 - https://dx.doi.org/10.1007/s12035-013-8619-z DB - PRIME DP - Unbound Medicine ER -