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Transcriptional activation of ColE1 DNA synthesis by displacement of the nontranscribed strand.
Cell. 1987 Dec 24; 51(6):1123-30.Cell

Abstract

Plasmid ColE1 can replicate using RNAase H and DNA polymerase I. However, it can also replicate in the absence of these enzymes. In this case, formation of a persistent hybrid between a transcript (RNA II) and the DNA indirectly activates subsequent DNA synthesis, instead of providing a primer as it does in the presence of these enzymes. To activate DNA synthesis, a certain length is required for the hybridized region and the region of minimum length cannot include a palindrome. These results show that the single-stranded region of DNA displaced by the hybridization is responsible for the activation. A single-stranded region was identified on the nontranscribed strand by its enhanced reactivity to dimethyl sulfate. The necessary length for the single-stranded region is at least 40 nucleotides. The region probably provides a site for initial binding of a helicase that further unwinds the template DNA for initiation of DNA synthesis.

Authors+Show Affiliations

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2446775

Citation

Masukata, H, et al. "Transcriptional Activation of ColE1 DNA Synthesis By Displacement of the Nontranscribed Strand." Cell, vol. 51, no. 6, 1987, pp. 1123-30.
Masukata H, Dasgupta S, Tomizawa J. Transcriptional activation of ColE1 DNA synthesis by displacement of the nontranscribed strand. Cell. 1987;51(6):1123-30.
Masukata, H., Dasgupta, S., & Tomizawa, J. (1987). Transcriptional activation of ColE1 DNA synthesis by displacement of the nontranscribed strand. Cell, 51(6), 1123-30.
Masukata H, Dasgupta S, Tomizawa J. Transcriptional Activation of ColE1 DNA Synthesis By Displacement of the Nontranscribed Strand. Cell. 1987 Dec 24;51(6):1123-30. PubMed PMID: 2446775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional activation of ColE1 DNA synthesis by displacement of the nontranscribed strand. AU - Masukata,H, AU - Dasgupta,S, AU - Tomizawa,J, PY - 1987/12/24/pubmed PY - 1987/12/24/medline PY - 1987/12/24/entrez SP - 1123 EP - 30 JF - Cell JO - Cell VL - 51 IS - 6 N2 - Plasmid ColE1 can replicate using RNAase H and DNA polymerase I. However, it can also replicate in the absence of these enzymes. In this case, formation of a persistent hybrid between a transcript (RNA II) and the DNA indirectly activates subsequent DNA synthesis, instead of providing a primer as it does in the presence of these enzymes. To activate DNA synthesis, a certain length is required for the hybridized region and the region of minimum length cannot include a palindrome. These results show that the single-stranded region of DNA displaced by the hybridization is responsible for the activation. A single-stranded region was identified on the nontranscribed strand by its enhanced reactivity to dimethyl sulfate. The necessary length for the single-stranded region is at least 40 nucleotides. The region probably provides a site for initial binding of a helicase that further unwinds the template DNA for initiation of DNA synthesis. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/2446775/Transcriptional_activation_of_ColE1_DNA_synthesis_by_displacement_of_the_nontranscribed_strand_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(87)90598-8 DB - PRIME DP - Unbound Medicine ER -