Tags

Type your tag names separated by a space and hit enter

Identification of the DRw10 DR beta 1-chain allele as encoding a polymorphic class II major histocompatibility complex epitope otherwise restricted to DR beta 2 molecules of the DRw53 type.
J Immunol. 1988 Jan 15; 140(2):537-43.JI

Abstract

Although the polymorphic human Ia epitope recognized by monoclonal antibody 109d6 typically is expressed by DRw53 beta 2 chains, the epitope was shown to be encoded by distinctive DR beta 1 chains of a DRw10 haplotype in three unrelated DR4-negative individuals with rheumatoid arthritis. No evidence of a DR beta 2 (DR beta 4) chain molecule was found to be encoded by this haplotype. Using two-dimensional gel analysis and partial radioactive N-terminal microsequencing, the DR and DQ products were characterized in the heterozygous members of a family in which the segregation of both varieties of DR beta chains specifying the 109d6 epitope was demonstrated. The expression of the epitope on the DR beta 2 chain, but not on the DR beta 1 chain, was abolished by preventing N-linked glycosylation, although in both molecules the epitope was not altered by neuraminidase digestion. The potential structural bases of the serologic cross reactions of DRw10 are discussed, as are the possible implications of the findings for the definition of susceptibility to rheumatoid arthritis.

Authors+Show Affiliations

Department of Rheumatic Diseases, Hospital for Joint Diseases, New York 10003.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2447181

Citation

Matsuyama, T, et al. "Identification of the DRw10 DR Beta 1-chain Allele as Encoding a Polymorphic Class II Major Histocompatibility Complex Epitope Otherwise Restricted to DR Beta 2 Molecules of the DRw53 Type." Journal of Immunology (Baltimore, Md. : 1950), vol. 140, no. 2, 1988, pp. 537-43.
Matsuyama T, Winchester R, Lee S, et al. Identification of the DRw10 DR beta 1-chain allele as encoding a polymorphic class II major histocompatibility complex epitope otherwise restricted to DR beta 2 molecules of the DRw53 type. J Immunol. 1988;140(2):537-43.
Matsuyama, T., Winchester, R., Lee, S., Shookster, L., & Nunez-Roldan, A. (1988). Identification of the DRw10 DR beta 1-chain allele as encoding a polymorphic class II major histocompatibility complex epitope otherwise restricted to DR beta 2 molecules of the DRw53 type. Journal of Immunology (Baltimore, Md. : 1950), 140(2), 537-43.
Matsuyama T, et al. Identification of the DRw10 DR Beta 1-chain Allele as Encoding a Polymorphic Class II Major Histocompatibility Complex Epitope Otherwise Restricted to DR Beta 2 Molecules of the DRw53 Type. J Immunol. 1988 Jan 15;140(2):537-43. PubMed PMID: 2447181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the DRw10 DR beta 1-chain allele as encoding a polymorphic class II major histocompatibility complex epitope otherwise restricted to DR beta 2 molecules of the DRw53 type. AU - Matsuyama,T, AU - Winchester,R, AU - Lee,S, AU - Shookster,L, AU - Nunez-Roldan,A, PY - 1988/1/15/pubmed PY - 1988/1/15/medline PY - 1988/1/15/entrez SP - 537 EP - 43 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 140 IS - 2 N2 - Although the polymorphic human Ia epitope recognized by monoclonal antibody 109d6 typically is expressed by DRw53 beta 2 chains, the epitope was shown to be encoded by distinctive DR beta 1 chains of a DRw10 haplotype in three unrelated DR4-negative individuals with rheumatoid arthritis. No evidence of a DR beta 2 (DR beta 4) chain molecule was found to be encoded by this haplotype. Using two-dimensional gel analysis and partial radioactive N-terminal microsequencing, the DR and DQ products were characterized in the heterozygous members of a family in which the segregation of both varieties of DR beta chains specifying the 109d6 epitope was demonstrated. The expression of the epitope on the DR beta 2 chain, but not on the DR beta 1 chain, was abolished by preventing N-linked glycosylation, although in both molecules the epitope was not altered by neuraminidase digestion. The potential structural bases of the serologic cross reactions of DRw10 are discussed, as are the possible implications of the findings for the definition of susceptibility to rheumatoid arthritis. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2447181/Identification_of_the_DRw10_DR_beta_1_chain_allele_as_encoding_a_polymorphic_class_II_major_histocompatibility_complex_epitope_otherwise_restricted_to_DR_beta_2_molecules_of_the_DRw53_type_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2447181 DB - PRIME DP - Unbound Medicine ER -