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Comparison of Plasmagel with LeucoPREP-Macrodex methods for separation of leukocytes for virus isolation.
Diagn Microbiol Infect Dis. 1987 Oct; 8(2):123-6.DM

Abstract

Plasmagel (Cellular Products, Inc., Buffalo, NY), which can separate both polymorphonuclear leukocytes (PMN) and mononuclear cells from other blood components, and LeucoPREP (Becton Dickinson Immunocytometry Systems, Mountain View, CA), which can separate mononuclear cells from other blood components, were used to harvest leukocytes from whole blood for the purpose of virus isolation. Macrodex was combined with the later, in a second step, for recovery of PMN. Of 90 peripheral blood specimens examined, cytomegalovirus was recovered from 10: in six by both methods, in three from Plasmagel prepared cells only, and in one from cells from the LeucoPREP-Macrodex preparation only. Total leukocyte counts, differential counts, and leukocyte viability did not differ significantly for the two methods. Plasmagel provided an efficient, inexpensive means of harvesting leukocytes from whole blood for virus isolation.

Authors+Show Affiliations

Department of Microbiology, Cleveland Clinic Foundation, OH 44106.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2448079

Citation

Woods, G L., and M R. Proffitt. "Comparison of Plasmagel With LeucoPREP-Macrodex Methods for Separation of Leukocytes for Virus Isolation." Diagnostic Microbiology and Infectious Disease, vol. 8, no. 2, 1987, pp. 123-6.
Woods GL, Proffitt MR. Comparison of Plasmagel with LeucoPREP-Macrodex methods for separation of leukocytes for virus isolation. Diagn Microbiol Infect Dis. 1987;8(2):123-6.
Woods, G. L., & Proffitt, M. R. (1987). Comparison of Plasmagel with LeucoPREP-Macrodex methods for separation of leukocytes for virus isolation. Diagnostic Microbiology and Infectious Disease, 8(2), 123-6.
Woods GL, Proffitt MR. Comparison of Plasmagel With LeucoPREP-Macrodex Methods for Separation of Leukocytes for Virus Isolation. Diagn Microbiol Infect Dis. 1987;8(2):123-6. PubMed PMID: 2448079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Plasmagel with LeucoPREP-Macrodex methods for separation of leukocytes for virus isolation. AU - Woods,G L, AU - Proffitt,M R, PY - 1987/10/1/pubmed PY - 1987/10/1/medline PY - 1987/10/1/entrez SP - 123 EP - 6 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 8 IS - 2 N2 - Plasmagel (Cellular Products, Inc., Buffalo, NY), which can separate both polymorphonuclear leukocytes (PMN) and mononuclear cells from other blood components, and LeucoPREP (Becton Dickinson Immunocytometry Systems, Mountain View, CA), which can separate mononuclear cells from other blood components, were used to harvest leukocytes from whole blood for the purpose of virus isolation. Macrodex was combined with the later, in a second step, for recovery of PMN. Of 90 peripheral blood specimens examined, cytomegalovirus was recovered from 10: in six by both methods, in three from Plasmagel prepared cells only, and in one from cells from the LeucoPREP-Macrodex preparation only. Total leukocyte counts, differential counts, and leukocyte viability did not differ significantly for the two methods. Plasmagel provided an efficient, inexpensive means of harvesting leukocytes from whole blood for virus isolation. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/2448079/Comparison_of_Plasmagel_with_LeucoPREP-Macrodex_methods_for_separation_of_leukocytes_for_virus_isolation L2 - https://linkinghub.elsevier.com/retrieve/pii/0732-8893(87)90160-X DB - PRIME DP - Unbound Medicine ER -