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Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model.
Pharmacol Biochem Behav. 2014 May; 120:57-64.PB

Abstract

BACKGROUND

Resveratrol is a polyphone chemical found in a number of plant species, including peanuts and grapes, but with significant amounts in red wine. In normal plant physiology, Resveratrol is produced as a defensive response to injury or parasitic attacks. Resveratrol has diverse biological properties and actions with potential clinical applications, including anti-inflammatory, antioxidant, anti proliferative, and neuroprotective effects.

AIM

The aim of the present study was to explore the effect and mechanism of Resveratrol in Pentylenetetrazole (PTZ) induced kindling in rats.

MATERIALS AND METHODS

In a PTZ kindled Wistar rat model, different doses of Resveratrol (25mg/kg, 50mg/kg and 75 mg/kg) were administered orally 30 min before the PTZ injection. The PTZ injection was given on alternate day till the animal became fully kindled or till 10 weeks. The following parameters were compared between control and various experimental groups: the course of kindling, stages of seizures, histopathological scoring of hippocampus, antioxidant parameters, DNA fragmentation and caspase-3 expression in the hippocampus, and neuron-specific enolase in the blood. One way ANOVA followed by Bonferroni post hoc analysis and Fischer's Exact test were used for statistical analyses.

THE RESULTS

In the present study, Resveratrol showed dose-dependent anti-seizure effect. Resveratrol (75 mg/kg) significantly increased the latency to myoclonic jerks, clinic seizures as well as generalized tonic-clinic seizures, improved the seizure score and decreased the number of myoclonic jerks. PTZ induced kindling caused a significant neuronal injury, oxidative stress and apoptosis which were reversed by pretreatment with Resveratrol in a dose-dependent manner.

CONCLUSION

Our study suggests that Resveratrol has a potential antiepileptogenic effect on PTZ-induced kindling in rats. The possible underlying mechanisms of Resveratrol as an antiepileptic agent may be due to its antioxidative property and neuroprotective effect.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Saha L
Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India. Electronic address: lekhasaha@rediffmail.com.
Chakrabarti A
Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India.

MeSH

AnimalsAnticonvulsantsAntioxidantsCaspase 3ConvulsantsHippocampusKindling, NeurologicLipid PeroxidationMaleOxidative StressPentylenetetrazolePhosphopyruvate HydrataseRatsResveratrolSeizuresStilbenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24486908

Citation

Saha, Lekha, and Amitava Chakrabarti. "Understanding the Anti-kindling Role and Its Mechanism of Resveratrol in Pentylenetetrazole Induced-kindling in a Rat Model." Pharmacology, Biochemistry, and Behavior, vol. 120, 2014, pp. 57-64.
Saha L, Chakrabarti A. Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model. Pharmacol Biochem Behav. 2014;120:57-64.
Saha, L., & Chakrabarti, A. (2014). Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model. Pharmacology, Biochemistry, and Behavior, 120, 57-64. https://doi.org/10.1016/j.pbb.2014.01.010
Saha L, Chakrabarti A. Understanding the Anti-kindling Role and Its Mechanism of Resveratrol in Pentylenetetrazole Induced-kindling in a Rat Model. Pharmacol Biochem Behav. 2014;120:57-64. PubMed PMID: 24486908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model. AU - Saha,Lekha, AU - Chakrabarti,Amitava, Y1 - 2014/01/30/ PY - 2013/11/08/received PY - 2014/01/18/revised PY - 2014/01/22/accepted PY - 2014/2/4/entrez PY - 2014/2/4/pubmed PY - 2014/12/15/medline KW - Antioxidant KW - Epileptogenesis KW - Kindling KW - Pentylenetetrazole KW - Rat KW - Resveratrol SP - 57 EP - 64 JF - Pharmacology, biochemistry, and behavior JO - Pharmacol Biochem Behav VL - 120 N2 - BACKGROUND: Resveratrol is a polyphone chemical found in a number of plant species, including peanuts and grapes, but with significant amounts in red wine. In normal plant physiology, Resveratrol is produced as a defensive response to injury or parasitic attacks. Resveratrol has diverse biological properties and actions with potential clinical applications, including anti-inflammatory, antioxidant, anti proliferative, and neuroprotective effects. AIM: The aim of the present study was to explore the effect and mechanism of Resveratrol in Pentylenetetrazole (PTZ) induced kindling in rats. MATERIALS AND METHODS: In a PTZ kindled Wistar rat model, different doses of Resveratrol (25mg/kg, 50mg/kg and 75 mg/kg) were administered orally 30 min before the PTZ injection. The PTZ injection was given on alternate day till the animal became fully kindled or till 10 weeks. The following parameters were compared between control and various experimental groups: the course of kindling, stages of seizures, histopathological scoring of hippocampus, antioxidant parameters, DNA fragmentation and caspase-3 expression in the hippocampus, and neuron-specific enolase in the blood. One way ANOVA followed by Bonferroni post hoc analysis and Fischer's Exact test were used for statistical analyses. THE RESULTS: In the present study, Resveratrol showed dose-dependent anti-seizure effect. Resveratrol (75 mg/kg) significantly increased the latency to myoclonic jerks, clinic seizures as well as generalized tonic-clinic seizures, improved the seizure score and decreased the number of myoclonic jerks. PTZ induced kindling caused a significant neuronal injury, oxidative stress and apoptosis which were reversed by pretreatment with Resveratrol in a dose-dependent manner. CONCLUSION: Our study suggests that Resveratrol has a potential antiepileptogenic effect on PTZ-induced kindling in rats. The possible underlying mechanisms of Resveratrol as an antiepileptic agent may be due to its antioxidative property and neuroprotective effect. SN - 1873-5177 UR - https://www.unboundmedicine.com/medline/citation/24486908/Understanding_the_anti_kindling_role_and_its_mechanism_of_Resveratrol_in_Pentylenetetrazole_induced_kindling_in_a_rat_model_ DB - PRIME DP - Unbound Medicine ER -