Tags

Type your tag names separated by a space and hit enter

Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold.
Biosens Bioelectron. 2014 Jun 15; 56:174-9.BB

Abstract

In this work, a sandwich-format electrochemical immunosensor has been fabricated in the aim of simultaneous detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) using metal ions tagged immunocolloidal gold nanocomposites as signal tags. The capture anti-CEA and anti-AFP were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) membrane modified glassy carbon electrode through glutaraldehyde (GA). The metal ion labels could be detected directly through differential pulse voltammetry (DPV) without metal pre-concentration, and the distinct voltammetric peaks had a close relationship with each sandwich-type immunoreaction. Under the optimized conditions, the multiplexed immunoassay exhibited good sensitivity and selectivity for the simultaneous determination of CEA and AFP with linear ranges of 0.01-50 ng mL(-1). The detection limits for CEA and AFP are 4.6 pg mL(-1) and 3.1 pg mL(-1), respectively. The method was successfully applied for the determination of AFP and CEA levels in clinical serum samples, and the results were in good agreement with standard enzyme linked immunosorbent assay (ELISA). This approach gives a promising simple and sensitive immunoassay strategy for clinical applications.

Authors+Show Affiliations

Department of Chemistry, Capital Normal University, Beijing 100048, China.Department of Chemistry, Capital Normal University, Beijing 100048, China.Department of Chemistry, Capital Normal University, Beijing 100048, China.Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24487105

Citation

Xu, Teng, et al. "Simultaneous Electrochemical Detection of Multiple Tumor Markers Using Metal Ions Tagged Immunocolloidal Gold." Biosensors & Bioelectronics, vol. 56, 2014, pp. 174-9.
Xu T, Jia X, Chen X, et al. Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold. Biosens Bioelectron. 2014;56:174-9.
Xu, T., Jia, X., Chen, X., & Ma, Z. (2014). Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold. Biosensors & Bioelectronics, 56, 174-9. https://doi.org/10.1016/j.bios.2014.01.006
Xu T, et al. Simultaneous Electrochemical Detection of Multiple Tumor Markers Using Metal Ions Tagged Immunocolloidal Gold. Biosens Bioelectron. 2014 Jun 15;56:174-9. PubMed PMID: 24487105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous electrochemical detection of multiple tumor markers using metal ions tagged immunocolloidal gold. AU - Xu,Teng, AU - Jia,Xinle, AU - Chen,Xia, AU - Ma,Zhanfang, Y1 - 2014/01/09/ PY - 2013/10/29/received PY - 2013/12/23/revised PY - 2014/01/03/accepted PY - 2014/2/4/entrez PY - 2014/2/4/pubmed PY - 2014/10/24/medline KW - Alpha-fetoprotein KW - Carcinoembryonic antigen KW - Chitosan–Au nanoparticles KW - Immunocolloidal gold KW - Metal ion labels KW - Multiplexed electrochemical detection SP - 174 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 56 N2 - In this work, a sandwich-format electrochemical immunosensor has been fabricated in the aim of simultaneous detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) using metal ions tagged immunocolloidal gold nanocomposites as signal tags. The capture anti-CEA and anti-AFP were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) membrane modified glassy carbon electrode through glutaraldehyde (GA). The metal ion labels could be detected directly through differential pulse voltammetry (DPV) without metal pre-concentration, and the distinct voltammetric peaks had a close relationship with each sandwich-type immunoreaction. Under the optimized conditions, the multiplexed immunoassay exhibited good sensitivity and selectivity for the simultaneous determination of CEA and AFP with linear ranges of 0.01-50 ng mL(-1). The detection limits for CEA and AFP are 4.6 pg mL(-1) and 3.1 pg mL(-1), respectively. The method was successfully applied for the determination of AFP and CEA levels in clinical serum samples, and the results were in good agreement with standard enzyme linked immunosorbent assay (ELISA). This approach gives a promising simple and sensitive immunoassay strategy for clinical applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24487105/Simultaneous_electrochemical_detection_of_multiple_tumor_markers_using_metal_ions_tagged_immunocolloidal_gold_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00009-8 DB - PRIME DP - Unbound Medicine ER -