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p-Synephrine suppresses lipopolysaccharide-induced acute lung injury by inhibition of the NF-κB signaling pathway.
Inflamm Res 2014; 63(6):429-39IR

Abstract

OBJECTIVE

We investigated whether p-synephrine exerts potent anti-inflammatory effects against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in vivo, and we further investigated the inhibitory mechanism of p-synephrine in LPS-induced ALI.

METHODS

Lipopolysaccharide (0.5 mg/kg) was instilled intranasally in phosphate-buffered saline to induce acute lung injury, and 6, 24, and 48 h after LPS was given, bronchoalveolar lavage fluid was obtained to measure pro-inflammatory mediator. We also evaluated the effects of p-synephrine on LPS-induced the severity of pulmonary injury. The phosphorylation of nuclear factor-κB (NF-κB) p65 protein was analyzed by Western blotting.

RESULTS

Our data showed that p-synephrine significantly reduced the amount of inflammatory cells, the lung wet-to-dry weight (W/D) ratio, reactive oxygen species, myeloperoxidase activity and enhanced superoxide dismutase (SOD) in mice with LPS-induced ALI. Tumor necrosis factor α and interleukin (IL)-6 concentrations decreased significantly while the concentration of IL-10 was significantly increased after p-synephrine pretreatment. In addition, p-synephrine suppressed not only the phosphorylation of NF-κB but also the degradation of its inhibitor (IκBα).

CONCLUSIONS

These results suggested that the inhibition of NF-κB activation and the regulation of SOD are involved in the mechanism of p-synephrine's protection against ALI.

Authors+Show Affiliations

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24487736

Citation

Wu, Qianchao, et al. "P-Synephrine Suppresses Lipopolysaccharide-induced Acute Lung Injury By Inhibition of the NF-κB Signaling Pathway." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 63, no. 6, 2014, pp. 429-39.
Wu Q, Li R, Soromou LW, et al. P-Synephrine suppresses lipopolysaccharide-induced acute lung injury by inhibition of the NF-κB signaling pathway. Inflamm Res. 2014;63(6):429-39.
Wu, Q., Li, R., Soromou, L. W., Chen, N., Yuan, X., Sun, G., ... Feng, H. (2014). P-Synephrine suppresses lipopolysaccharide-induced acute lung injury by inhibition of the NF-κB signaling pathway. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 63(6), pp. 429-39. doi:10.1007/s00011-014-0715-7.
Wu Q, et al. P-Synephrine Suppresses Lipopolysaccharide-induced Acute Lung Injury By Inhibition of the NF-κB Signaling Pathway. Inflamm Res. 2014;63(6):429-39. PubMed PMID: 24487736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - p-Synephrine suppresses lipopolysaccharide-induced acute lung injury by inhibition of the NF-κB signaling pathway. AU - Wu,Qianchao, AU - Li,Ruisheng, AU - Soromou,Lanan Wassy, AU - Chen,Na, AU - Yuan,Xue, AU - Sun,Guoquan, AU - Li,Beibei, AU - Feng,Haihua, Y1 - 2014/02/01/ PY - 2013/06/05/received PY - 2014/01/22/accepted PY - 2013/12/06/revised PY - 2014/2/4/entrez PY - 2014/2/4/pubmed PY - 2015/1/28/medline SP - 429 EP - 39 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 63 IS - 6 N2 - OBJECTIVE: We investigated whether p-synephrine exerts potent anti-inflammatory effects against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in vivo, and we further investigated the inhibitory mechanism of p-synephrine in LPS-induced ALI. METHODS: Lipopolysaccharide (0.5 mg/kg) was instilled intranasally in phosphate-buffered saline to induce acute lung injury, and 6, 24, and 48 h after LPS was given, bronchoalveolar lavage fluid was obtained to measure pro-inflammatory mediator. We also evaluated the effects of p-synephrine on LPS-induced the severity of pulmonary injury. The phosphorylation of nuclear factor-κB (NF-κB) p65 protein was analyzed by Western blotting. RESULTS: Our data showed that p-synephrine significantly reduced the amount of inflammatory cells, the lung wet-to-dry weight (W/D) ratio, reactive oxygen species, myeloperoxidase activity and enhanced superoxide dismutase (SOD) in mice with LPS-induced ALI. Tumor necrosis factor α and interleukin (IL)-6 concentrations decreased significantly while the concentration of IL-10 was significantly increased after p-synephrine pretreatment. In addition, p-synephrine suppressed not only the phosphorylation of NF-κB but also the degradation of its inhibitor (IκBα). CONCLUSIONS: These results suggested that the inhibition of NF-κB activation and the regulation of SOD are involved in the mechanism of p-synephrine's protection against ALI. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/24487736/p_Synephrine_suppresses_lipopolysaccharide_induced_acute_lung_injury_by_inhibition_of_the_NF_κB_signaling_pathway_ L2 - https://dx.doi.org/10.1007/s00011-014-0715-7 DB - PRIME DP - Unbound Medicine ER -