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Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles.
Pest Manag Sci. 2014 Oct; 70(10):1588-94.PM

Abstract

BACKGROUND

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most invasive and destructive pests of field crops worldwide. The sibling species B and Q are the two most damaging members of the B. tabaci species complex. That Q is more resistant than B to many insecticides has been well documented. Over the last decade, Q has gradually displaced B and has become the dominant form of B. tabaci in field agricultural systems in most parts of China. To help understand the differences in insecticide resistance, the activities and gene expression profiles of detoxification enzymes in B. tabaci B and Q were investigated.

RESULTS

The activity of P450 towards 7-ethoxycoumarin was significantly higher (1.46-fold higher) in Q than in B. The expression of 43 of 65 P450 genes was higher (>1-fold) in Q than in B, and expression for eight P450 genes was more than 50-fold greater in Q than in B. The increased expression of selected P450 genes in Q relative to B was confirmed with two other B strains and two other Q strains. On the other hand, carboxylesterase (CarE) activity was significantly lower (0.71-fold lower) in Q than in B; the Km value of CarE was significantly lower in B than in Q, but the opposite was true for the Vmax value of CarE. Glutathione S-transferase activity and values of Km and Vmax did not differ between B and Q.

CONCLUSION

Enhanced metabolic detoxification of insecticides by P450s may be an important reason why B. tabaci Q is more resistant than B. tabaci B to insecticides.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Guo L
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Xie W
No affiliation info available
Wang S
No affiliation info available
Wu Q
No affiliation info available
Li R
No affiliation info available
Yang N
No affiliation info available
Yang X
No affiliation info available
Pan H
No affiliation info available
Zhang Y
No affiliation info available

MeSH

AnimalsCarboxylesteraseChinaCoumarinsCytochrome P-450 Enzyme SystemGlutathione TransferaseHemipteraInsecticide ResistanceInsecticidesTranscriptome

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24488614

Citation

Guo, Litao, et al. "Detoxification Enzymes of Bemisia Tabaci B and Q: Biochemical Characteristics and Gene Expression Profiles." Pest Management Science, vol. 70, no. 10, 2014, pp. 1588-94.
Guo L, Xie W, Wang S, et al. Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles. Pest Manag Sci. 2014;70(10):1588-94.
Guo, L., Xie, W., Wang, S., Wu, Q., Li, R., Yang, N., Yang, X., Pan, H., & Zhang, Y. (2014). Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles. Pest Management Science, 70(10), 1588-94. https://doi.org/10.1002/ps.3751
Guo L, et al. Detoxification Enzymes of Bemisia Tabaci B and Q: Biochemical Characteristics and Gene Expression Profiles. Pest Manag Sci. 2014;70(10):1588-94. PubMed PMID: 24488614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles. AU - Guo,Litao, AU - Xie,Wen, AU - Wang,Shaoli, AU - Wu,Qingjun, AU - Li,Rumei, AU - Yang,Nina, AU - Yang,Xin, AU - Pan,Huipeng, AU - Zhang,Youjun, Y1 - 2014/04/08/ PY - 2013/11/19/received PY - 2014/01/28/accepted PY - 2014/2/4/entrez PY - 2014/2/4/pubmed PY - 2015/5/13/medline KW - Bemisia tabaci KW - carboxylesterase KW - cytochrome-P450-dependent monooxygenase KW - detoxification enzymes KW - glutathione S-transferase KW - quantitative real-time PCR analysis SP - 1588 EP - 94 JF - Pest management science JO - Pest Manag Sci VL - 70 IS - 10 N2 - BACKGROUND: The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most invasive and destructive pests of field crops worldwide. The sibling species B and Q are the two most damaging members of the B. tabaci species complex. That Q is more resistant than B to many insecticides has been well documented. Over the last decade, Q has gradually displaced B and has become the dominant form of B. tabaci in field agricultural systems in most parts of China. To help understand the differences in insecticide resistance, the activities and gene expression profiles of detoxification enzymes in B. tabaci B and Q were investigated. RESULTS: The activity of P450 towards 7-ethoxycoumarin was significantly higher (1.46-fold higher) in Q than in B. The expression of 43 of 65 P450 genes was higher (>1-fold) in Q than in B, and expression for eight P450 genes was more than 50-fold greater in Q than in B. The increased expression of selected P450 genes in Q relative to B was confirmed with two other B strains and two other Q strains. On the other hand, carboxylesterase (CarE) activity was significantly lower (0.71-fold lower) in Q than in B; the Km value of CarE was significantly lower in B than in Q, but the opposite was true for the Vmax value of CarE. Glutathione S-transferase activity and values of Km and Vmax did not differ between B and Q. CONCLUSION: Enhanced metabolic detoxification of insecticides by P450s may be an important reason why B. tabaci Q is more resistant than B. tabaci B to insecticides. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/24488614/Detoxification_enzymes_of_Bemisia_tabaci_B_and_Q:_biochemical_characteristics_and_gene_expression_profiles_ DB - PRIME DP - Unbound Medicine ER -
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