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Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3.
FEBS Lett. 2014 Mar 03; 588(5):789-94.FL

Abstract

Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity.

Authors+Show Affiliations

Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan.Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572 Ibaraki, Japan.Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan. Electronic address: akagi@akita-pu.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24492003

Citation

Kumagai, Saori, et al. "Functional Analysis of the C-terminal Region of the Vacuolar Cadmium-transporting Rice OsHMA3." FEBS Letters, vol. 588, no. 5, 2014, pp. 789-94.
Kumagai S, Suzuki T, Tezuka K, et al. Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3. FEBS Lett. 2014;588(5):789-94.
Kumagai, S., Suzuki, T., Tezuka, K., Satoh-Nagasawa, N., Takahashi, H., Sakurai, K., Watanabe, A., Fujimura, T., & Akagi, H. (2014). Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3. FEBS Letters, 588(5), 789-94. https://doi.org/10.1016/j.febslet.2014.01.037
Kumagai S, et al. Functional Analysis of the C-terminal Region of the Vacuolar Cadmium-transporting Rice OsHMA3. FEBS Lett. 2014 Mar 3;588(5):789-94. PubMed PMID: 24492003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3. AU - Kumagai,Saori, AU - Suzuki,Tatsuya, AU - Tezuka,Kouichi, AU - Satoh-Nagasawa,Namiko, AU - Takahashi,Hidekazu, AU - Sakurai,Kenji, AU - Watanabe,Akio, AU - Fujimura,Tatsuhito, AU - Akagi,Hiromori, Y1 - 2014/02/01/ PY - 2013/12/01/received PY - 2014/01/09/revised PY - 2014/01/17/accepted PY - 2014/2/5/entrez PY - 2014/2/5/pubmed PY - 2014/4/15/medline KW - C-terminal domain KW - Cadmium KW - OsHMA3 KW - P(1B)-ATPase (HMA) KW - Rice KW - Vacuolar sequestration SP - 789 EP - 94 JF - FEBS letters JO - FEBS Lett VL - 588 IS - 5 N2 - Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/24492003/Functional_analysis_of_the_C_terminal_region_of_the_vacuolar_cadmium_transporting_rice_OsHMA3_ DB - PRIME DP - Unbound Medicine ER -