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Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds.
BMC Complement Altern Med. 2014 Feb 04; 14:45.BC

Abstract

BACKGROUND

Antioxidant compounds like phenols and flavonoids scavenge free radicals and thus inhibit the oxidative mechanisms that lead to control degenerative and other diseases. The aim of this study was to investigate the antioxidant activity in vitro, total phenolic and flavonoid contents in ethanol extracts and fractions of Crescentia cujete leaves and stem bark.

METHODS

Crescentia cujete leaves and bark crude ethanol extract (CEE) and their partitionates petroleum ether (PEF), chloroform (CHF), ethyl acetate (EAF) and aqueous (AQF) were firstly prepared. Different established testing methods, such as 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical, ferric reducing power (FRP), and total antioxidant capacity (TAC) assays were used to detect the antioxidant activity. Further, the total yield, total phenolic (TPC) and total flavonoid contents (TFC) of CEE and all the fractions were determined. Ethanol extracts of both leaves and stem bark were also subjected to preliminary phytochemical screening to detect the presence of secondary metabolites, using standard phytochemical methods (Thin layer chromatography and spray reagents).

RESULTS

Phytochemical screening of crude ethanol extract of both leaves and stem bark revealed the presence of steroids, flavonoids, saponins, tannins, glycosides and terpenoids. All the fractions and CEE of leaves and bark exhibited antioxidant activities, however, EAF of leaves showing the highest antioxidant activity based on the results of DPPH, FRP and TAC assay tests. The above fraction has shown the significant DPPH scavenging activity (IC50 = 8.78 μg/ml) when compared with standard ascorbic acid (IC50 =7.68 μg/ml). The TAC and FRP activities increased with increasing crude extract/fractions content. The TPC (371.23 ± 15.77 mg GAE/g extract) and TFC (144.64 ± 5.82 mg QE/g extract) of EAF of leaves were found significantly higher as compared to other solvent fractions for both leaves and bark. TPC were highly correlated with the antioxidant activity (R2 = 0.9268 and 0.8515 in DPPH test for leaves and bark, respectively).

CONCLUSION

The results of the study show that leaves of C. cujete possesses significant free radical scavenging properties compared with stem bark and a clear correlation exists between the antioxidant activity and phenolic content.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableDepartment of Pharmacy, Rajshahi University, Rajshahi 6205, Bangladesh. shahnaj_parvin@ru.ac.bd.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24495381

Citation

Das, Nandita, et al. "Antioxidant Activities of Ethanol Extracts and Fractions of Crescentia Cujete Leaves and Stem Bark and the Involvement of Phenolic Compounds." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 45.
Das N, Islam ME, Jahan N, et al. Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complement Altern Med. 2014;14:45.
Das, N., Islam, M. E., Jahan, N., Islam, M. S., Khan, A., Islam, M. R., & Parvin, M. S. (2014). Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complementary and Alternative Medicine, 14, 45. https://doi.org/10.1186/1472-6882-14-45
Das N, et al. Antioxidant Activities of Ethanol Extracts and Fractions of Crescentia Cujete Leaves and Stem Bark and the Involvement of Phenolic Compounds. BMC Complement Altern Med. 2014 Feb 4;14:45. PubMed PMID: 24495381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. AU - Das,Nandita, AU - Islam,Md Ekramul, AU - Jahan,Nusrat, AU - Islam,Mohammad Saiful, AU - Khan,Alam, AU - Islam,Md Rafikul, AU - Parvin,Mst Shahnaj, Y1 - 2014/02/04/ PY - 2013/08/09/received PY - 2014/01/29/accepted PY - 2014/2/6/entrez PY - 2014/2/6/pubmed PY - 2015/10/9/medline SP - 45 EP - 45 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Antioxidant compounds like phenols and flavonoids scavenge free radicals and thus inhibit the oxidative mechanisms that lead to control degenerative and other diseases. The aim of this study was to investigate the antioxidant activity in vitro, total phenolic and flavonoid contents in ethanol extracts and fractions of Crescentia cujete leaves and stem bark. METHODS: Crescentia cujete leaves and bark crude ethanol extract (CEE) and their partitionates petroleum ether (PEF), chloroform (CHF), ethyl acetate (EAF) and aqueous (AQF) were firstly prepared. Different established testing methods, such as 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical, ferric reducing power (FRP), and total antioxidant capacity (TAC) assays were used to detect the antioxidant activity. Further, the total yield, total phenolic (TPC) and total flavonoid contents (TFC) of CEE and all the fractions were determined. Ethanol extracts of both leaves and stem bark were also subjected to preliminary phytochemical screening to detect the presence of secondary metabolites, using standard phytochemical methods (Thin layer chromatography and spray reagents). RESULTS: Phytochemical screening of crude ethanol extract of both leaves and stem bark revealed the presence of steroids, flavonoids, saponins, tannins, glycosides and terpenoids. All the fractions and CEE of leaves and bark exhibited antioxidant activities, however, EAF of leaves showing the highest antioxidant activity based on the results of DPPH, FRP and TAC assay tests. The above fraction has shown the significant DPPH scavenging activity (IC50 = 8.78 μg/ml) when compared with standard ascorbic acid (IC50 =7.68 μg/ml). The TAC and FRP activities increased with increasing crude extract/fractions content. The TPC (371.23 ± 15.77 mg GAE/g extract) and TFC (144.64 ± 5.82 mg QE/g extract) of EAF of leaves were found significantly higher as compared to other solvent fractions for both leaves and bark. TPC were highly correlated with the antioxidant activity (R2 = 0.9268 and 0.8515 in DPPH test for leaves and bark, respectively). CONCLUSION: The results of the study show that leaves of C. cujete possesses significant free radical scavenging properties compared with stem bark and a clear correlation exists between the antioxidant activity and phenolic content. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24495381/Antioxidant_activities_of_ethanol_extracts_and_fractions_of_Crescentia_cujete_leaves_and_stem_bark_and_the_involvement_of_phenolic_compounds_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-45 DB - PRIME DP - Unbound Medicine ER -