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Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages.
J Ethnopharmacol. 2014 Jun 11; 154(2):311-8.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE AND AIM OF THE STUDY

Phellinus linteus is a herb used in traditional Asian medicine to treat stomachache, inflammation, and tumors. Recent studies show that the extract of Phellinus linteus has anti-inflammatory and antitumor activities. However, Phellinus linteus extract has limitation of high cost and limited availability because of supply shortage. Here, we grew Phellinus linteus on germinated brown rice to address the issue of supply shortage and investigated anti-inflammatory effect in vivo as well as in vitro.

MATERIALS AND METHODS

Phellinus linteus grown on germinated brown rice (PBR) were extracted using filtration steps, which included γ-aminobutyric acid (GABA). The PBR (200, 500mg/kg/day) was applied into the mouse model of dextran sodium sulfate (DSS)-induced colitis and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. We used sulfasalazine as a reference drug. In addition, mechanism related to anti-inflammatory was investigated by Western blotting.

RESULTS

In the mouse model of DSS-induced colitis, PBR ameliorated the pathological characteristics of colitis such as shortening of colon length and improved the disease activity index score. In addition, we showed that PBR reduced the expression of nuclear factor-kappa B (NF-κB) in colitis. Western blotting showed that PBR decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins. Further, PBR treatment reduced the expression of mitogen-activated protein kinases (MAPKs) (e.g., extracellular signal-regulated protein kinase (ERK) and p38) in the mouse model of DSS-induced colitis.

CONCLUSIONS

Treatment of RAW 264.7 macrophages with a combination of PBR and LPS showed a significant concentration-dependent inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) production. In addition, we determined the ability of PBR to reduce the iNOS and tumor necrosis factor (TNF)-α expression. PBR inhibited the expression of iNOS, NF-κB, and Cox-2 proteins in LPS-stimulated RAW264.7 macrophages. This study presents the potential use of PBR as a drug candidate against colitis.

Authors+Show Affiliations

Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-Gu, Achasan-rho 263, Seoul, South Korea.Department of Food science and Biotechnology, College of BioNano technology, Gachon University, Sungnam 461-701, South Korea. Electronic address: nimpi79@hanmail.net.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24495471

Citation

Song, Minjung, and Hye-Jin Park. "Anti-inflammatory Effect of Phellinus Linteus Grown On Germinated Brown Rice On Dextran Sodium Sulfate-induced Acute Colitis in Mice and LPS-activated Macrophages." Journal of Ethnopharmacology, vol. 154, no. 2, 2014, pp. 311-8.
Song M, Park HJ. Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages. J Ethnopharmacol. 2014;154(2):311-8.
Song, M., & Park, H. J. (2014). Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages. Journal of Ethnopharmacology, 154(2), 311-8. https://doi.org/10.1016/j.jep.2013.12.059
Song M, Park HJ. Anti-inflammatory Effect of Phellinus Linteus Grown On Germinated Brown Rice On Dextran Sodium Sulfate-induced Acute Colitis in Mice and LPS-activated Macrophages. J Ethnopharmacol. 2014 Jun 11;154(2):311-8. PubMed PMID: 24495471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages. AU - Song,Minjung, AU - Park,Hye-Jin, Y1 - 2014/02/01/ PY - 2013/09/04/received PY - 2013/12/07/revised PY - 2013/12/31/accepted PY - 2014/2/6/entrez PY - 2014/2/6/pubmed PY - 2015/1/7/medline KW - Acute colitis KW - Anti-inflammatory KW - Dextran sodium sulfate (DSS) KW - Macrophages KW - Phellinus linteus KW - Phellinus linteus grown on germinated brown rice SP - 311 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 154 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE AND AIM OF THE STUDY: Phellinus linteus is a herb used in traditional Asian medicine to treat stomachache, inflammation, and tumors. Recent studies show that the extract of Phellinus linteus has anti-inflammatory and antitumor activities. However, Phellinus linteus extract has limitation of high cost and limited availability because of supply shortage. Here, we grew Phellinus linteus on germinated brown rice to address the issue of supply shortage and investigated anti-inflammatory effect in vivo as well as in vitro. MATERIALS AND METHODS: Phellinus linteus grown on germinated brown rice (PBR) were extracted using filtration steps, which included γ-aminobutyric acid (GABA). The PBR (200, 500mg/kg/day) was applied into the mouse model of dextran sodium sulfate (DSS)-induced colitis and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. We used sulfasalazine as a reference drug. In addition, mechanism related to anti-inflammatory was investigated by Western blotting. RESULTS: In the mouse model of DSS-induced colitis, PBR ameliorated the pathological characteristics of colitis such as shortening of colon length and improved the disease activity index score. In addition, we showed that PBR reduced the expression of nuclear factor-kappa B (NF-κB) in colitis. Western blotting showed that PBR decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins. Further, PBR treatment reduced the expression of mitogen-activated protein kinases (MAPKs) (e.g., extracellular signal-regulated protein kinase (ERK) and p38) in the mouse model of DSS-induced colitis. CONCLUSIONS: Treatment of RAW 264.7 macrophages with a combination of PBR and LPS showed a significant concentration-dependent inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) production. In addition, we determined the ability of PBR to reduce the iNOS and tumor necrosis factor (TNF)-α expression. PBR inhibited the expression of iNOS, NF-κB, and Cox-2 proteins in LPS-stimulated RAW264.7 macrophages. This study presents the potential use of PBR as a drug candidate against colitis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24495471/Anti_inflammatory_effect_of_Phellinus_linteus_grown_on_germinated_brown_rice_on_dextran_sodium_sulfate_induced_acute_colitis_in_mice_and_LPS_activated_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(14)00009-9 DB - PRIME DP - Unbound Medicine ER -