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A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples.
Acta Trop 2015; 141(Pt B):170-7AT

Abstract

Although schistosomiasis remains a serious health problem worldwide, significant achievements in schistosomiasis control has been made in the People's Republic of China. The disease has been eliminated in five out of 12 endemic provinces, and the prevalence in remaining endemic areas is very low and is heading toward elimination. A rapid and sensitive method for monitoring the distribution of infected Oncomelania hupensis is urgently required. We applied a loop-mediated isothermal amplification (LAMP) assay targeting 28S rDNA for the rapid and effective detection of Schistosoma japonicum DNA in infected and prepatent infected O. hupensis snails. The detection limit of the LAMP method was 100 fg of S. japonicum genomic DNA. To promote the application of the approach in the field, the LAMP assay was used to detect infection in pooled samples of field-collected snails. In the pooled sample detection, snails were collected from 28 endemic areas, and 50 snails from each area were pooled based on the maximum pool size estimation, crushed together and DNA was extracted from each pooled sample as template for the LAMP assay. Based on the formula for detection from pooled samples, the proportion of positive pooled samples and the positive proportion of O. hupensis detected by LAMP of Xima village reached 66.67% and 1.33%, while those of Heini, Hongjia, Yangjiang and Huangshan villages were 33.33% and 0.67%, and those of Tuanzhou and Suliao villages were 16.67% and 0.33%, respectively. The remaining 21 monitoring field sites gave negative results. A risk map for the transmission of schistosomiasis was constructed using ArcMap, based on the positive proportion of O. hupensis infected with S. japonicum, as detected by the LAMP assay, which will form a guide for surveillance and response strategies in high risk areas.

Authors+Show Affiliations

Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China.Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, P.R. China; Key Laboratory for Parasite and Vector Biology, MOH, P.R. China.Jiangsu Institute of Schistosomiasis, Wuxi, P.R. China.Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China.Jiangsu Institute of Schistosomiasis, Wuxi, P.R. China.Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China.Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China. Electronic address: llsshh2003@163.com.Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, P.R. China; Key Laboratory for Parasite and Vector Biology, MOH, P.R. China. Electronic address: ipdzhouxn@sh163.net.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

24495631

Citation

Tong, Qun-Bo, et al. "A New Surveillance and Response Tool: Risk Map of Infected Oncomelania Hupensis Detected By Loop-mediated Isothermal Amplification (LAMP) From Pooled Samples." Acta Tropica, vol. 141, no. Pt B, 2015, pp. 170-7.
Tong QB, Chen R, Zhang Y, et al. A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples. Acta Trop. 2015;141(Pt B):170-7.
Tong, Q. B., Chen, R., Zhang, Y., Yang, G. J., Kumagai, T., Furushima-Shimogawara, R., ... Zhou, X. N. (2015). A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples. Acta Tropica, 141(Pt B), pp. 170-7. doi:10.1016/j.actatropica.2014.01.006.
Tong QB, et al. A New Surveillance and Response Tool: Risk Map of Infected Oncomelania Hupensis Detected By Loop-mediated Isothermal Amplification (LAMP) From Pooled Samples. Acta Trop. 2015;141(Pt B):170-7. PubMed PMID: 24495631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples. AU - Tong,Qun-Bo, AU - Chen,Rui, AU - Zhang,Yi, AU - Yang,Guo-Jing, AU - Kumagai,Takashi, AU - Furushima-Shimogawara,Rieko, AU - Lou,Di, AU - Yang,Kun, AU - Wen,Li-Yong, AU - Lu,Shao-Hong, AU - Ohta,Nobuo, AU - Zhou,Xiao-Nong, Y1 - 2014/02/02/ PY - 2013/03/08/received PY - 2014/01/16/revised PY - 2014/01/21/accepted PY - 2014/2/6/entrez PY - 2014/2/6/pubmed PY - 2016/1/13/medline KW - Loop-mediated isothermal amplification (LAMP) KW - Oncomelania hupensis KW - Risk map KW - Schistosoma japonicum KW - Surveillance and response SP - 170 EP - 7 JF - Acta tropica JO - Acta Trop. VL - 141 IS - Pt B N2 - Although schistosomiasis remains a serious health problem worldwide, significant achievements in schistosomiasis control has been made in the People's Republic of China. The disease has been eliminated in five out of 12 endemic provinces, and the prevalence in remaining endemic areas is very low and is heading toward elimination. A rapid and sensitive method for monitoring the distribution of infected Oncomelania hupensis is urgently required. We applied a loop-mediated isothermal amplification (LAMP) assay targeting 28S rDNA for the rapid and effective detection of Schistosoma japonicum DNA in infected and prepatent infected O. hupensis snails. The detection limit of the LAMP method was 100 fg of S. japonicum genomic DNA. To promote the application of the approach in the field, the LAMP assay was used to detect infection in pooled samples of field-collected snails. In the pooled sample detection, snails were collected from 28 endemic areas, and 50 snails from each area were pooled based on the maximum pool size estimation, crushed together and DNA was extracted from each pooled sample as template for the LAMP assay. Based on the formula for detection from pooled samples, the proportion of positive pooled samples and the positive proportion of O. hupensis detected by LAMP of Xima village reached 66.67% and 1.33%, while those of Heini, Hongjia, Yangjiang and Huangshan villages were 33.33% and 0.67%, and those of Tuanzhou and Suliao villages were 16.67% and 0.33%, respectively. The remaining 21 monitoring field sites gave negative results. A risk map for the transmission of schistosomiasis was constructed using ArcMap, based on the positive proportion of O. hupensis infected with S. japonicum, as detected by the LAMP assay, which will form a guide for surveillance and response strategies in high risk areas. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/24495631/A_new_surveillance_and_response_tool:_risk_map_of_infected_Oncomelania_hupensis_detected_by_Loop_mediated_isothermal_amplification__LAMP__from_pooled_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(14)00011-4 DB - PRIME DP - Unbound Medicine ER -