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Molecular epidemiology of Orientia tsutsugamushi in chiggers and ticks from domestic rodents in Shandong, northern China.
Parasit Vectors 2013; 6(1):312PV

Abstract

BACKGROUND

Scrub typhus is endemic to a 13,000,000-km² area of the Asia-Pacific region, and causes an annual incidence of 1 million people. The mortality rate of scrub typhus ranges from 6.1% to 25% in Southeast Asia. Natural infection of Orientia tsutsugamushi has been identified in domestic rodents in Shandong Province. However, infestation of chiggers and ticks on the domestic rodents and prevalence and genotypes of O. tsutsugamushi in these Acarina remain unclear.

METHODS

During September 2010 to March 2012, 3134 chiggers and 89 ticks were collected from domestic rodents captured in three counties of Shandong Province. We amplified and sequenced the 56-kDa type-specific antigen gene of O. tsutsugamushi from DNA samples of these Acarina and designated to genotype according to sequence analysis.

RESULTS

Overall, the infestation rate of chiggers on domestic rodents was 17.0%, and the chigger index was 5.38. The infestation rate of ticks on rodents was 3.1%. Natural infection of O. tsutsugamushi was found in Leptotrombidium taishanicum, L. linhuaikongense, L. intermedium, L. scutellare, L. palpale, and Ixodes spp., the minimum positive rates of which were 5.9%, 3.2%, 1.2%, 0.8%, 0.8%, and 2.2%, respectively. Kawasaki-like genotypes were predominant in chiggers and ticks on domestic rodents, which were detected from L. taishanicum, L. intermedium, L. scutellare, L. palpale, and Ixodes spp. Shimokoshi-like genotype was detected from L. palpale.

CONCLUSIONS

In the present study we investigated the infestation of chiggers and ticks on domestic rodents in Shandong Province, and identified the prevalence and genotypes of O. tsutsugamushi in the Acarina. Infestation of vector chiggers in domestic rodents, prevalence of O. tsutsugamushi in infested chiggers, and high nucleotide homologies among the O. tsutsugamushi sequences from the Acarina, their animal hosts and scrub typhus patients, implied that domestic rodents may play an important role in the transmission of scrub typhus in Shandong, China. Further studies are needed to verify the vector significance of chiggers and ticks that tested positive for O. tsutsugamushi, and to assess the risk of human exposure to chiggers and ticks on domestic rodents.

Authors+Show Affiliations

No affiliation info availableDepartment of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan, China. ztzhao@sdu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24499627

Citation

Zhang, Meng, et al. "Molecular Epidemiology of Orientia Tsutsugamushi in Chiggers and Ticks From Domestic Rodents in Shandong, Northern China." Parasites & Vectors, vol. 6, no. 1, 2013, p. 312.
Zhang M, Zhao ZT, Yang HL, et al. Molecular epidemiology of Orientia tsutsugamushi in chiggers and ticks from domestic rodents in Shandong, northern China. Parasit Vectors. 2013;6(1):312.
Zhang, M., Zhao, Z. T., Yang, H. L., Zhang, A. H., Xu, X. Q., Meng, X. P., ... Zhang, L. Y. (2013). Molecular epidemiology of Orientia tsutsugamushi in chiggers and ticks from domestic rodents in Shandong, northern China. Parasites & Vectors, 6(1), p. 312. doi:10.1186/1756-3305-6-312.
Zhang M, et al. Molecular Epidemiology of Orientia Tsutsugamushi in Chiggers and Ticks From Domestic Rodents in Shandong, Northern China. Parasit Vectors. 2013 Oct 29;6(1):312. PubMed PMID: 24499627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of Orientia tsutsugamushi in chiggers and ticks from domestic rodents in Shandong, northern China. AU - Zhang,Meng, AU - Zhao,Zhong-Tang, AU - Yang,Hui-Li, AU - Zhang,Ai-Hua, AU - Xu,Xing-Qu, AU - Meng,Xiang-Peng, AU - Zhang,Hai-Yu, AU - Wang,Xian-Jun, AU - Li,Zhong, AU - Ding,Shu-Jun, AU - Yang,Li, AU - Zhang,Lu-Yan, Y1 - 2013/10/29/ PY - 2013/08/12/received PY - 2013/10/20/accepted PY - 2014/2/7/entrez PY - 2014/2/7/pubmed PY - 2014/8/30/medline SP - 312 EP - 312 JF - Parasites & vectors JO - Parasit Vectors VL - 6 IS - 1 N2 - BACKGROUND: Scrub typhus is endemic to a 13,000,000-km² area of the Asia-Pacific region, and causes an annual incidence of 1 million people. The mortality rate of scrub typhus ranges from 6.1% to 25% in Southeast Asia. Natural infection of Orientia tsutsugamushi has been identified in domestic rodents in Shandong Province. However, infestation of chiggers and ticks on the domestic rodents and prevalence and genotypes of O. tsutsugamushi in these Acarina remain unclear. METHODS: During September 2010 to March 2012, 3134 chiggers and 89 ticks were collected from domestic rodents captured in three counties of Shandong Province. We amplified and sequenced the 56-kDa type-specific antigen gene of O. tsutsugamushi from DNA samples of these Acarina and designated to genotype according to sequence analysis. RESULTS: Overall, the infestation rate of chiggers on domestic rodents was 17.0%, and the chigger index was 5.38. The infestation rate of ticks on rodents was 3.1%. Natural infection of O. tsutsugamushi was found in Leptotrombidium taishanicum, L. linhuaikongense, L. intermedium, L. scutellare, L. palpale, and Ixodes spp., the minimum positive rates of which were 5.9%, 3.2%, 1.2%, 0.8%, 0.8%, and 2.2%, respectively. Kawasaki-like genotypes were predominant in chiggers and ticks on domestic rodents, which were detected from L. taishanicum, L. intermedium, L. scutellare, L. palpale, and Ixodes spp. Shimokoshi-like genotype was detected from L. palpale. CONCLUSIONS: In the present study we investigated the infestation of chiggers and ticks on domestic rodents in Shandong Province, and identified the prevalence and genotypes of O. tsutsugamushi in the Acarina. Infestation of vector chiggers in domestic rodents, prevalence of O. tsutsugamushi in infested chiggers, and high nucleotide homologies among the O. tsutsugamushi sequences from the Acarina, their animal hosts and scrub typhus patients, implied that domestic rodents may play an important role in the transmission of scrub typhus in Shandong, China. Further studies are needed to verify the vector significance of chiggers and ticks that tested positive for O. tsutsugamushi, and to assess the risk of human exposure to chiggers and ticks on domestic rodents. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/24499627/Molecular_epidemiology_of_Orientia_tsutsugamushi_in_chiggers_and_ticks_from_domestic_rodents_in_Shandong_northern_China_ L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-6-312 DB - PRIME DP - Unbound Medicine ER -