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Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR.
Diagn Microbiol Infect Dis. 2014 Apr; 78(4):333-7.DM

Abstract

Mycoplasma pneumoniae is a common cause of community-acquired pneumonia, which is often empirically treated with macrolides such as erythromycin and azithromycin. Recent studies have found a gradual increase in the numbers of macrolide-resistant strains due to mutations in the 23S rRNA gene. A2063G is the most common mutation, followed by A2064G. We developed a Cycleave PCR method for detecting macrolide-resistant strains of M. pneumoniae. With use of this method, the resistant strains can be quickly separated from the susceptible ones. In this work, via this method, both M. pneumoniae and resistance mutants were successfully identified from 101 clinical isolates as well as from 136 nasopharyngeal specimens. The findings of this study may allow clinicians to determine the treatment plan more rapidly and may provide a convenient method to conduct surveillance for genetic mutations conferring antibiotic resistance.

Authors+Show Affiliations

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, 200040, PR China.Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China.Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai 200040, PR China. Electronic address: xuxiaogang@fudan.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24503503

Citation

Liu, Yang, et al. "Rapid Detection of Mycoplasma Pneumoniae and Its Macrolide-resistance Mutation By Cycleave PCR." Diagnostic Microbiology and Infectious Disease, vol. 78, no. 4, 2014, pp. 333-7.
Liu Y, Ye X, Zhang H, et al. Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR. Diagn Microbiol Infect Dis. 2014;78(4):333-7.
Liu, Y., Ye, X., Zhang, H., Wu, Z., & Xu, X. (2014). Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR. Diagnostic Microbiology and Infectious Disease, 78(4), 333-7. https://doi.org/10.1016/j.diagmicrobio.2013.12.002
Liu Y, et al. Rapid Detection of Mycoplasma Pneumoniae and Its Macrolide-resistance Mutation By Cycleave PCR. Diagn Microbiol Infect Dis. 2014;78(4):333-7. PubMed PMID: 24503503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR. AU - Liu,Yang, AU - Ye,Xinye, AU - Zhang,Hong, AU - Wu,Zhen, AU - Xu,Xiaogang, Y1 - 2013/12/13/ PY - 2013/04/30/received PY - 2013/12/03/revised PY - 2013/12/05/accepted PY - 2014/2/8/entrez PY - 2014/2/8/pubmed PY - 2014/11/5/medline KW - CycleavePCR KW - Detect KW - Macrolide resistance KW - Mycoplasma pneumoniae SP - 333 EP - 7 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 78 IS - 4 N2 - Mycoplasma pneumoniae is a common cause of community-acquired pneumonia, which is often empirically treated with macrolides such as erythromycin and azithromycin. Recent studies have found a gradual increase in the numbers of macrolide-resistant strains due to mutations in the 23S rRNA gene. A2063G is the most common mutation, followed by A2064G. We developed a Cycleave PCR method for detecting macrolide-resistant strains of M. pneumoniae. With use of this method, the resistant strains can be quickly separated from the susceptible ones. In this work, via this method, both M. pneumoniae and resistance mutants were successfully identified from 101 clinical isolates as well as from 136 nasopharyngeal specimens. The findings of this study may allow clinicians to determine the treatment plan more rapidly and may provide a convenient method to conduct surveillance for genetic mutations conferring antibiotic resistance. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/24503503/Rapid_detection_of_Mycoplasma_pneumoniae_and_its_macrolide_resistance_mutation_by_Cycleave_PCR_ DB - PRIME DP - Unbound Medicine ER -