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[Investigation of antimicrobial and antibiofilm effects of some preservatives used in drugs, cosmetics and food products].
Mikrobiyol Bul. 2014 Jan; 48(1):94-105.MB

Abstract

Preservatives are added to food, drugs and other pharmaceutical products to avoid microbial contamination. For antimicrobial activity testing and preservative efficacy testing, vegetative forms of the standard test organisms are used. However, microbial biofilm formation may occur on living tissues, medical implants, industrial or drinking water pipes, natural aquatic systems, glass and plastic surfaces. In our study, it was aimed to determine the antimicrobial and antibiofilm effects of some preservatives used in drug, cosmetics and food products and to compare the minimum biofilm inhibitory concentration (MBIC) of microbial biofilm formed on glass surfaces which are commonly used in those areas and the minimum inhibitory concentration (MIC) values of the planktonic forms. In the study Pseudomonas aeruginosa ATCC 27853, Salmonella Thyphimurium SL1344, Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis NCTC 11047, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 10231 were used as the standard strains; sodium nitrate, methylparaben, prophylparaben, potassium sorbate and sodium benzoate as the preservatives; ampicillin, vancomycin, gentamicin, ciprofloxacin, amphotericin B and itraconazole as the antimicrobial agents. MIC values were determined through the guidelines of CLSI M100-S18 and M27-A3 protocols. BioTimer method was used to determine the MBIC values. The value of "colony forming unit (CFU)/glass beads" was calculated using the graphics drawn by plotting the time of color change for phenol red or resazurin against log10CFU. All experiments were done with four media at different pH values namely pH: 7, pH: 6.5, pH: 6 and pH: 5.5. According to the results of tests on planktonic forms of the microorganisms, sodium benzoate was determined to be the most effective preservative against all the microorganisms tested except S.aureus and E.faecalis. The most effective preservative against S.aureus and E.faecalis was prophylparaben. Prophylparaben was also effective against S.epidermidis. However, in our study it was determined that preservatives were not effective against biofilm forms even if the inoculum was lower, equal to or higher than the inocula of the planktonic forms. The data obtained from this study indicated that preservatives used to prevent microbial contamination in pharmaceutical, cosmetic and food products, are not effective against biofilm forms of the microorganisms. This study is thought to be a guide for further studies to be held in the investigation of antimicrobial and antibiofilm effects of preservatives used in drugs, cosmetics and food industry.

Authors+Show Affiliations

No affiliation info availableTrakya University Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Edirne, Turkey. fatmakaynak@trakya.edu.tr.

Pub Type(s)

English Abstract
Journal Article

Language

tur

PubMed ID

24506719

Citation

Güven, Nihal, and Fatma Kaynak Onurdağ. "[Investigation of Antimicrobial and Antibiofilm Effects of some Preservatives Used in Drugs, Cosmetics and Food Products]." Mikrobiyoloji Bulteni, vol. 48, no. 1, 2014, pp. 94-105.
Güven N, Kaynak Onurdağ F. [Investigation of antimicrobial and antibiofilm effects of some preservatives used in drugs, cosmetics and food products]. Mikrobiyol Bul. 2014;48(1):94-105.
Güven, N., & Kaynak Onurdağ, F. (2014). [Investigation of antimicrobial and antibiofilm effects of some preservatives used in drugs, cosmetics and food products]. Mikrobiyoloji Bulteni, 48(1), 94-105.
Güven N, Kaynak Onurdağ F. [Investigation of Antimicrobial and Antibiofilm Effects of some Preservatives Used in Drugs, Cosmetics and Food Products]. Mikrobiyol Bul. 2014;48(1):94-105. PubMed PMID: 24506719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Investigation of antimicrobial and antibiofilm effects of some preservatives used in drugs, cosmetics and food products]. AU - Güven,Nihal, AU - Kaynak Onurdağ,Fatma, PY - 2014/2/11/entrez PY - 2014/2/11/pubmed PY - 2014/6/13/medline SP - 94 EP - 105 JF - Mikrobiyoloji bulteni JO - Mikrobiyol Bul VL - 48 IS - 1 N2 - Preservatives are added to food, drugs and other pharmaceutical products to avoid microbial contamination. For antimicrobial activity testing and preservative efficacy testing, vegetative forms of the standard test organisms are used. However, microbial biofilm formation may occur on living tissues, medical implants, industrial or drinking water pipes, natural aquatic systems, glass and plastic surfaces. In our study, it was aimed to determine the antimicrobial and antibiofilm effects of some preservatives used in drug, cosmetics and food products and to compare the minimum biofilm inhibitory concentration (MBIC) of microbial biofilm formed on glass surfaces which are commonly used in those areas and the minimum inhibitory concentration (MIC) values of the planktonic forms. In the study Pseudomonas aeruginosa ATCC 27853, Salmonella Thyphimurium SL1344, Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis NCTC 11047, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 10231 were used as the standard strains; sodium nitrate, methylparaben, prophylparaben, potassium sorbate and sodium benzoate as the preservatives; ampicillin, vancomycin, gentamicin, ciprofloxacin, amphotericin B and itraconazole as the antimicrobial agents. MIC values were determined through the guidelines of CLSI M100-S18 and M27-A3 protocols. BioTimer method was used to determine the MBIC values. The value of "colony forming unit (CFU)/glass beads" was calculated using the graphics drawn by plotting the time of color change for phenol red or resazurin against log10CFU. All experiments were done with four media at different pH values namely pH: 7, pH: 6.5, pH: 6 and pH: 5.5. According to the results of tests on planktonic forms of the microorganisms, sodium benzoate was determined to be the most effective preservative against all the microorganisms tested except S.aureus and E.faecalis. The most effective preservative against S.aureus and E.faecalis was prophylparaben. Prophylparaben was also effective against S.epidermidis. However, in our study it was determined that preservatives were not effective against biofilm forms even if the inoculum was lower, equal to or higher than the inocula of the planktonic forms. The data obtained from this study indicated that preservatives used to prevent microbial contamination in pharmaceutical, cosmetic and food products, are not effective against biofilm forms of the microorganisms. This study is thought to be a guide for further studies to be held in the investigation of antimicrobial and antibiofilm effects of preservatives used in drugs, cosmetics and food industry. SN - 0374-9096 UR - https://www.unboundmedicine.com/medline/citation/24506719/[Investigation_of_antimicrobial_and_antibiofilm_effects_of_some_preservatives_used_in_drugs_cosmetics_and_food_products]_ L2 - http://www.mikrobiyolbul.org/linkout.aspx?pmid=24506719 DB - PRIME DP - Unbound Medicine ER -