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Dehydroabietic acid (DHAA) alters metabolic enzyme activity and the effects of 17β-estradiol in rainbow trout (Oncorhynchus mykiss).

Abstract

Recent studies have shown that dehydroabietic acid (DHAA), a resin acid present in pulp and paper mill effluent, affects liver energy metabolism and may have anti-estrogenic effects in fish. A chronic-exposure toxicity experiment using immature rainbow trout (Oncorhynchus mykiss) was conducted in order to assess the endocrine disrupting and liver metabolic effects of the model estrogen 17β-estradiol (E2) and the wood extractives DHAA and β-sitosterol (BS), regularly present in pulp and paper mill effluents. Exposure to 5ppm of E2 significantly increased hepatosomatic index (HSI), vitellogenin (VTG) and plasma sorbitol dehydrogenase (SDH). This latter effect was reduced by mixing E2 with DHAA, indicating that DHAA does not cause its endocrine disrupting effects indirectly due to liver damage. Exposure to 0.5ppm of DHAA as well as all the DHAA mixed treatments caused significant increases in liver citrate synthase (CS), activity after 7 days, however, the fish returned to control values by 28 days. Results indicate that DHAA may alter metabolic enzyme activity as well as alter the effects of E2 in juvenile rainbow trout.

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  • Authors+Show Affiliations

    ,

    University of Ontario Institute of Technology, Oshawa, Ontario, Canada L1H 7K4.

    ,

    University of Ontario Institute of Technology, Oshawa, Ontario, Canada L1H 7K4.

    University of Ontario Institute of Technology, Oshawa, Ontario, Canada L1H 7K4. Electronic address: Douglas.Holdway@uoit.ca.

    Source

    MeSH

    Animals
    Citrate (si)-Synthase
    Cytochrome P-450 CYP1A1
    Diterpenes, Abietane
    Drug Interactions
    Enzyme Activation
    Estradiol
    L-Iditol 2-Dehydrogenase
    Liver
    Oncorhynchus mykiss
    Vitellogenins
    Water Pollutants, Chemical

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    24507142

    Citation

    Pandelides, Z, et al. "Dehydroabietic Acid (DHAA) Alters Metabolic Enzyme Activity and the Effects of 17β-estradiol in Rainbow Trout (Oncorhynchus Mykiss)." Ecotoxicology and Environmental Safety, vol. 101, 2014, pp. 168-76.
    Pandelides Z, Guchardi J, Holdway D. Dehydroabietic acid (DHAA) alters metabolic enzyme activity and the effects of 17β-estradiol in rainbow trout (Oncorhynchus mykiss). Ecotoxicol Environ Saf. 2014;101:168-76.
    Pandelides, Z., Guchardi, J., & Holdway, D. (2014). Dehydroabietic acid (DHAA) alters metabolic enzyme activity and the effects of 17β-estradiol in rainbow trout (Oncorhynchus mykiss). Ecotoxicology and Environmental Safety, 101, pp. 168-76. doi:10.1016/j.ecoenv.2013.11.027.
    Pandelides Z, Guchardi J, Holdway D. Dehydroabietic Acid (DHAA) Alters Metabolic Enzyme Activity and the Effects of 17β-estradiol in Rainbow Trout (Oncorhynchus Mykiss). Ecotoxicol Environ Saf. 2014;101:168-76. PubMed PMID: 24507142.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Dehydroabietic acid (DHAA) alters metabolic enzyme activity and the effects of 17β-estradiol in rainbow trout (Oncorhynchus mykiss). AU - Pandelides,Z, AU - Guchardi,J, AU - Holdway,D, Y1 - 2014/01/15/ PY - 2013/06/19/received PY - 2013/11/12/revised PY - 2013/11/27/accepted PY - 2014/2/11/entrez PY - 2014/2/11/pubmed PY - 2014/3/29/medline KW - Chronic exposure KW - Dehydroabietic acid KW - Estradiol KW - Metabolic enzyme KW - Sorbitol dehydrogenase KW - Vitellogenin SP - 168 EP - 76 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 101 N2 - Recent studies have shown that dehydroabietic acid (DHAA), a resin acid present in pulp and paper mill effluent, affects liver energy metabolism and may have anti-estrogenic effects in fish. A chronic-exposure toxicity experiment using immature rainbow trout (Oncorhynchus mykiss) was conducted in order to assess the endocrine disrupting and liver metabolic effects of the model estrogen 17β-estradiol (E2) and the wood extractives DHAA and β-sitosterol (BS), regularly present in pulp and paper mill effluents. Exposure to 5ppm of E2 significantly increased hepatosomatic index (HSI), vitellogenin (VTG) and plasma sorbitol dehydrogenase (SDH). This latter effect was reduced by mixing E2 with DHAA, indicating that DHAA does not cause its endocrine disrupting effects indirectly due to liver damage. Exposure to 0.5ppm of DHAA as well as all the DHAA mixed treatments caused significant increases in liver citrate synthase (CS), activity after 7 days, however, the fish returned to control values by 28 days. Results indicate that DHAA may alter metabolic enzyme activity as well as alter the effects of E2 in juvenile rainbow trout. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/24507142/Dehydroabietic_acid_(DHAA)_alters_metabolic_enzyme_activity_and_the_effects_of_17β-estradiol_in_rainbow_trout_(Oncorhynchus_mykiss) L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(13)00522-8 DB - PRIME DP - Unbound Medicine ER -