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Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots.
BMC Complement Altern Med. 2014 Feb 08; 14:47.BC

Abstract

BACKGROUND

Roots of Rumex hastatus (Polygonaceae) are traditionally used for the treatment of various ailments including liver and lung diseases. In this study, various solvent extracts of R. hastatus roots, like methanolic, n-hexane, ethyl acetate, chloroform, butanol and aqueous fractions were assessed through their antioxidant properties in vitro and determination of phenolic contents.

METHODS

Several parameters like DPPH˙, ABTS˙(+), ˙OH, H2O2, superoxide free radical scavenging, iron chelating power, reducing power, β-carotene bleaching power, antioxidant capacity and total phenolics and flavonoids were evaluated. High Performance liquid Chromatography (HPLC) was also considered.

RESULTS

Though all the fractions exhibited dose dependant activity. The samples with the highest activity were the butanol and methanol fractions in all the assays except hydrogen peroxide radical scavenging assay where chloroform fraction showed the highest scavenging aptitude. On the other hand, aquous fraction showed significant beta carotene linoleic acid, while n-hexane and ethyl acetate fractions exhibited a lesser antioxidant activity in all the assays. HPLC revealed the presence of rutin, luteolin-7-glucoside, vitexin and luteolin.

CONCLUSION

These results have to some extent substantiated the use of R. hastatus roots against different diseases, as an excellent basis of potential antioxidant due to the presence of sufficient amount of phenolics such as rutin and luteolin.

Authors+Show Affiliations

Botanical Sciences Division, Pakistan Museum of Natural History, Garden Avenue, Shakarparian, Islamabad, Pakistan. sumairasahreen@gmail.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24507200

Citation

Sahreen, Sumaira, et al. "Comprehensive Assessment of Phenolics and Antiradical Potential of Rumex Hastatus D. Don. Roots." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 47.
Sahreen S, Khan MR, Khan RA. Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots. BMC Complement Altern Med. 2014;14:47.
Sahreen, S., Khan, M. R., & Khan, R. A. (2014). Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots. BMC Complementary and Alternative Medicine, 14, 47. https://doi.org/10.1186/1472-6882-14-47
Sahreen S, Khan MR, Khan RA. Comprehensive Assessment of Phenolics and Antiradical Potential of Rumex Hastatus D. Don. Roots. BMC Complement Altern Med. 2014 Feb 8;14:47. PubMed PMID: 24507200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots. AU - Sahreen,Sumaira, AU - Khan,Muhammad Rashid, AU - Khan,Rahmat Ali, Y1 - 2014/02/08/ PY - 2013/03/05/received PY - 2014/02/05/accepted PY - 2014/2/11/entrez PY - 2014/2/11/pubmed PY - 2015/10/9/medline SP - 47 EP - 47 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Roots of Rumex hastatus (Polygonaceae) are traditionally used for the treatment of various ailments including liver and lung diseases. In this study, various solvent extracts of R. hastatus roots, like methanolic, n-hexane, ethyl acetate, chloroform, butanol and aqueous fractions were assessed through their antioxidant properties in vitro and determination of phenolic contents. METHODS: Several parameters like DPPH˙, ABTS˙(+), ˙OH, H2O2, superoxide free radical scavenging, iron chelating power, reducing power, β-carotene bleaching power, antioxidant capacity and total phenolics and flavonoids were evaluated. High Performance liquid Chromatography (HPLC) was also considered. RESULTS: Though all the fractions exhibited dose dependant activity. The samples with the highest activity were the butanol and methanol fractions in all the assays except hydrogen peroxide radical scavenging assay where chloroform fraction showed the highest scavenging aptitude. On the other hand, aquous fraction showed significant beta carotene linoleic acid, while n-hexane and ethyl acetate fractions exhibited a lesser antioxidant activity in all the assays. HPLC revealed the presence of rutin, luteolin-7-glucoside, vitexin and luteolin. CONCLUSION: These results have to some extent substantiated the use of R. hastatus roots against different diseases, as an excellent basis of potential antioxidant due to the presence of sufficient amount of phenolics such as rutin and luteolin. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24507200/Comprehensive_assessment_of_phenolics_and_antiradical_potential_of_Rumex_hastatus_D__Don__roots_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-47 DB - PRIME DP - Unbound Medicine ER -