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Structural characteristics of water-soluble polysaccharides from Heracleum sosnowskyi Manden.
Carbohydr Polym. 2014 Feb 15; 102:521-8.CP

Abstract

Fractions containing arabinogalactan proteins (AGPs) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. Major units of their structure were elucidated using ion-exchange chromatography, gel chromatography, and NMR spectroscopy. The carbohydrate backbone of the polysaccharides consisted of 1,3-β-D-galactopyranosyl residues, whereas side chains of the branched region consisted of the residues of 1,6-β-galactopyranose, 1,5-α-L-arabinofuranose, 1,4-β-D-glucuronic acid, and 1,6-β-D-glucopyranose. The branching points were identified as 1,3,6-β-D-galactopyranose residues. Side chains were terminated with β-D-galactopyranose, α-L-arabinofuranose and α-L-rhamnopyranose. A significant part of the side-chain β-1,6-galactan was substituted at C6 by 4-OMe-β-D-glucuronic acid. A minor part of glucuronic acid was included in the α-Rhap-(1 → 4)-β-GlcA-(→ fragment. All the studied fractions contained 1,4-β-D-galacturonic acid as well.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shakhmatov EG
Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia.
Toukach PV
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 119991, Russia.
Kuznetsov SP
Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia.
Makarova EN
Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya Street 49, Syktyvkar 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.

MeSH

Chromatography, GelChromatography, Ion ExchangeHeracleumMagnetic Resonance SpectroscopyMolecular StructurePolysaccharidesSolubilityWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24507314

Citation

Shakhmatov, Evgeny G., et al. "Structural Characteristics of Water-soluble Polysaccharides From Heracleum Sosnowskyi Manden." Carbohydrate Polymers, vol. 102, 2014, pp. 521-8.
Shakhmatov EG, Toukach PV, Kuznetsov SP, et al. Structural characteristics of water-soluble polysaccharides from Heracleum sosnowskyi Manden. Carbohydr Polym. 2014;102:521-8.
Shakhmatov, E. G., Toukach, P. V., Kuznetsov, S. P., & Makarova, E. N. (2014). Structural characteristics of water-soluble polysaccharides from Heracleum sosnowskyi Manden. Carbohydrate Polymers, 102, 521-8. https://doi.org/10.1016/j.carbpol.2013.12.001
Shakhmatov EG, et al. Structural Characteristics of Water-soluble Polysaccharides From Heracleum Sosnowskyi Manden. Carbohydr Polym. 2014 Feb 15;102:521-8. PubMed PMID: 24507314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characteristics of water-soluble polysaccharides from Heracleum sosnowskyi Manden. AU - Shakhmatov,Evgeny G, AU - Toukach,Philip V, AU - Kuznetsov,Sergey P, AU - Makarova,Elena N, Y1 - 2013/12/08/ PY - 2013/08/23/received PY - 2013/11/18/revised PY - 2013/12/01/accepted PY - 2014/2/11/entrez PY - 2014/2/11/pubmed PY - 2014/10/18/medline KW - Arabinogalactan protein KW - Glucomannan KW - Heracleum sosnowskyi KW - NMR KW - Pectin KW - Structure SP - 521 EP - 8 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 102 N2 - Fractions containing arabinogalactan proteins (AGPs) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. Major units of their structure were elucidated using ion-exchange chromatography, gel chromatography, and NMR spectroscopy. The carbohydrate backbone of the polysaccharides consisted of 1,3-β-D-galactopyranosyl residues, whereas side chains of the branched region consisted of the residues of 1,6-β-galactopyranose, 1,5-α-L-arabinofuranose, 1,4-β-D-glucuronic acid, and 1,6-β-D-glucopyranose. The branching points were identified as 1,3,6-β-D-galactopyranose residues. Side chains were terminated with β-D-galactopyranose, α-L-arabinofuranose and α-L-rhamnopyranose. A significant part of the side-chain β-1,6-galactan was substituted at C6 by 4-OMe-β-D-glucuronic acid. A minor part of glucuronic acid was included in the α-Rhap-(1 → 4)-β-GlcA-(→ fragment. All the studied fractions contained 1,4-β-D-galacturonic acid as well. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/24507314/Structural_characteristics_of_water_soluble_polysaccharides_from_Heracleum_sosnowskyi_Manden_ DB - PRIME DP - Unbound Medicine ER -