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Nucleotide sequence of a DRw10 beta chain cDNA clone. Identity of the third D region with that of the DRw53 allele of the beta 2 locus and as the probable site encoding a polymorphic MHC class II epitope.
J Immunol. 1988 Apr 01; 140(7):2447-52.JI

Abstract

The nucleotide and inferred amino acid sequence of a DRw10 beta chain was obtained from cDNA clones isolated from a DR1, DRw10 heterozygous cell line. The sequence of this beta chain gene was distinctive, differing from those of all other defined DR types. The DRw10 beta chain gene was shown by transfection experiments to encode a polymorphic epitope recognized by mAb 109d6 that is also encoded by the DRw53 beta 2 chain gene. Comparison of the nucleotide sequence of both genes revealed that their third D regions (amino acids 67 to 73) were identical. This suggested first that the 109d6 epitope could be encoded by residues of this region, and second, that a putative gene conversion event transferred this sequence along with the information encoding the 109d6 epitope from a donor gene such as DRw53 beta 2. The sequence of the DRw10 beta chain gene was observed to be identical to that of clone pII beta 4 derived from the non-DR3 haplotype in the Raji cell line, which was also demonstrated to express the determinant recognized by antibody 109d6, suggesting that the typing of this cell line is HLA-DR3/DRw10. No evidence was found for the existence of a DR beta 2 chain gene product encoded by the DRw10 haplotype. The DRw10 haplotype was of particular interest because it was present along with a DR1 haplotype in the propositus who had rheumatoid arthritis, and was shared by the DR4-positive son of the propositus, who also had rheumatoid arthritis. This raised the possibility that the DRw10 haplotype, and most probably one or more specific conformations encoded by the DR beta chain, are involved in the definition of the disease susceptibility phenotype.

Authors+Show Affiliations

Department of Rheumatic Diseases, Hospital for Joint Diseases, New York 10003.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2450924

Citation

Merryman, P, et al. "Nucleotide Sequence of a DRw10 Beta Chain cDNA Clone. Identity of the Third D Region With That of the DRw53 Allele of the Beta 2 Locus and as the Probable Site Encoding a Polymorphic MHC Class II Epitope." Journal of Immunology (Baltimore, Md. : 1950), vol. 140, no. 7, 1988, pp. 2447-52.
Merryman P, Gregersen PK, Lee S, et al. Nucleotide sequence of a DRw10 beta chain cDNA clone. Identity of the third D region with that of the DRw53 allele of the beta 2 locus and as the probable site encoding a polymorphic MHC class II epitope. J Immunol. 1988;140(7):2447-52.
Merryman, P., Gregersen, P. K., Lee, S., Silver, J., Nuñez-Roldan, A., Crapper, R., & Winchester, R. (1988). Nucleotide sequence of a DRw10 beta chain cDNA clone. Identity of the third D region with that of the DRw53 allele of the beta 2 locus and as the probable site encoding a polymorphic MHC class II epitope. Journal of Immunology (Baltimore, Md. : 1950), 140(7), 2447-52.
Merryman P, et al. Nucleotide Sequence of a DRw10 Beta Chain cDNA Clone. Identity of the Third D Region With That of the DRw53 Allele of the Beta 2 Locus and as the Probable Site Encoding a Polymorphic MHC Class II Epitope. J Immunol. 1988 Apr 1;140(7):2447-52. PubMed PMID: 2450924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleotide sequence of a DRw10 beta chain cDNA clone. Identity of the third D region with that of the DRw53 allele of the beta 2 locus and as the probable site encoding a polymorphic MHC class II epitope. AU - Merryman,P, AU - Gregersen,P K, AU - Lee,S, AU - Silver,J, AU - Nuñez-Roldan,A, AU - Crapper,R, AU - Winchester,R, PY - 1988/4/1/pubmed PY - 1988/4/1/medline PY - 1988/4/1/entrez SP - 2447 EP - 52 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 140 IS - 7 N2 - The nucleotide and inferred amino acid sequence of a DRw10 beta chain was obtained from cDNA clones isolated from a DR1, DRw10 heterozygous cell line. The sequence of this beta chain gene was distinctive, differing from those of all other defined DR types. The DRw10 beta chain gene was shown by transfection experiments to encode a polymorphic epitope recognized by mAb 109d6 that is also encoded by the DRw53 beta 2 chain gene. Comparison of the nucleotide sequence of both genes revealed that their third D regions (amino acids 67 to 73) were identical. This suggested first that the 109d6 epitope could be encoded by residues of this region, and second, that a putative gene conversion event transferred this sequence along with the information encoding the 109d6 epitope from a donor gene such as DRw53 beta 2. The sequence of the DRw10 beta chain gene was observed to be identical to that of clone pII beta 4 derived from the non-DR3 haplotype in the Raji cell line, which was also demonstrated to express the determinant recognized by antibody 109d6, suggesting that the typing of this cell line is HLA-DR3/DRw10. No evidence was found for the existence of a DR beta 2 chain gene product encoded by the DRw10 haplotype. The DRw10 haplotype was of particular interest because it was present along with a DR1 haplotype in the propositus who had rheumatoid arthritis, and was shared by the DR4-positive son of the propositus, who also had rheumatoid arthritis. This raised the possibility that the DRw10 haplotype, and most probably one or more specific conformations encoded by the DR beta chain, are involved in the definition of the disease susceptibility phenotype. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2450924/Nucleotide_sequence_of_a_DRw10_beta_chain_cDNA_clone__Identity_of_the_third_D_region_with_that_of_the_DRw53_allele_of_the_beta_2_locus_and_as_the_probable_site_encoding_a_polymorphic_MHC_class_II_epitope_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2450924 DB - PRIME DP - Unbound Medicine ER -