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Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris.
Pest Manag Sci. 2015 Jan; 71(1):40-57.PM

Abstract

BACKGROUND

Chemical spray on cotton is almost an exclusive method for controlling tarnished plant bug (TPB), Lygus lineolaris. Frequent use of imidacloprid is a concern for neonicotinoid resistance in this key pest. Information of how and why TPB becomes less susceptible to imidacloprid is essential for effective monitoring and managing resistance.

RESULTS

Microarray analysis of 6688 genes in imidacloprid-selected TPB (Im1500FF) revealed 955 upregulated and 1277 downregulated (≥twofold) genes in Im1500FF, with 369 and 485 of them annotated. Five P450 and nine esterase genes were significantly upregulated, and only one esterase gene and no P450 genes were downregulated. Other upregulated genes include helicases, phosphodiesterases, ATPases and kinases. Pathway analyses identified 65 upregulated cDNAs that encode 51 different enzymes involved in 62 different pathways, including P450 and esterase genes for drug and xenobiotic metabolisms. Sixty-four downregulated cDNAs code only 17 enzymes that are associated with only 23 pathways mostly related to food digestion.

CONCLUSIONS

This study demonstrated a significant change in gene expression related to metabolic processes in imidacloprid-selected TPB, resulting in overexpression of P450 and esterase genes for potential excess detoxification and cross/multiple resistance development. The identification of these and other enzyme genes establishes a foundation to explore the complicity of potential imidacloprid resistance in TPB.

Authors+Show Affiliations

USDA-ARS-JWDSRC, Stoneville, MS, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24515672

Citation

Zhu, Yu Cheng, and Randall Luttrell. "Altered Gene Regulation and Potential Association With Metabolic Resistance Development to Imidacloprid in the Tarnished Plant Bug, Lygus Lineolaris." Pest Management Science, vol. 71, no. 1, 2015, pp. 40-57.
Zhu YC, Luttrell R. Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris. Pest Manag Sci. 2015;71(1):40-57.
Zhu, Y. C., & Luttrell, R. (2015). Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris. Pest Management Science, 71(1), 40-57. https://doi.org/10.1002/ps.3761
Zhu YC, Luttrell R. Altered Gene Regulation and Potential Association With Metabolic Resistance Development to Imidacloprid in the Tarnished Plant Bug, Lygus Lineolaris. Pest Manag Sci. 2015;71(1):40-57. PubMed PMID: 24515672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris. AU - Zhu,Yu Cheng, AU - Luttrell,Randall, Y1 - 2014/03/20/ PY - 2013/06/26/received PY - 2013/12/22/revised PY - 2014/02/04/accepted PY - 2014/2/12/entrez PY - 2014/2/12/pubmed PY - 2015/8/13/medline KW - Lygus lineolaris KW - P450 KW - cDNA KW - esterase KW - imidacloprid KW - microarray KW - resistance KW - susceptibility KW - tarnished plant bug SP - 40 EP - 57 JF - Pest management science JO - Pest Manag Sci VL - 71 IS - 1 N2 - BACKGROUND: Chemical spray on cotton is almost an exclusive method for controlling tarnished plant bug (TPB), Lygus lineolaris. Frequent use of imidacloprid is a concern for neonicotinoid resistance in this key pest. Information of how and why TPB becomes less susceptible to imidacloprid is essential for effective monitoring and managing resistance. RESULTS: Microarray analysis of 6688 genes in imidacloprid-selected TPB (Im1500FF) revealed 955 upregulated and 1277 downregulated (≥twofold) genes in Im1500FF, with 369 and 485 of them annotated. Five P450 and nine esterase genes were significantly upregulated, and only one esterase gene and no P450 genes were downregulated. Other upregulated genes include helicases, phosphodiesterases, ATPases and kinases. Pathway analyses identified 65 upregulated cDNAs that encode 51 different enzymes involved in 62 different pathways, including P450 and esterase genes for drug and xenobiotic metabolisms. Sixty-four downregulated cDNAs code only 17 enzymes that are associated with only 23 pathways mostly related to food digestion. CONCLUSIONS: This study demonstrated a significant change in gene expression related to metabolic processes in imidacloprid-selected TPB, resulting in overexpression of P450 and esterase genes for potential excess detoxification and cross/multiple resistance development. The identification of these and other enzyme genes establishes a foundation to explore the complicity of potential imidacloprid resistance in TPB. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/24515672/Altered_gene_regulation_and_potential_association_with_metabolic_resistance_development_to_imidacloprid_in_the_tarnished_plant_bug_Lygus_lineolaris_ L2 - https://doi.org/10.1002/ps.3761 DB - PRIME DP - Unbound Medicine ER -