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Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress.
Braz J Microbiol. 2013; 44(3):993-1000.BJ

Abstract

Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1Δ, sod1Δ, gsh1Δ, gtt1Δ and gtt2Δ) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1Δ, acquired tolerance when previously treated with 25 μg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod.

Authors+Show Affiliations

Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24516431

Citation

de Sá, Rafael A., et al. "Brazilian Propolis Protects Saccharomyces Cerevisiae Cells Against Oxidative Stress." Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], vol. 44, no. 3, 2013, pp. 993-1000.
de Sá RA, de Castro FA, Eleutherio EC, et al. Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress. Braz J Microbiol. 2013;44(3):993-1000.
de Sá, R. A., de Castro, F. A., Eleutherio, E. C., de Souza, R. M., da Silva, J. F., & Pereira, M. D. (2013). Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress. Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], 44(3), 993-1000. https://doi.org/10.1590/S1517-83822013005000062
de Sá RA, et al. Brazilian Propolis Protects Saccharomyces Cerevisiae Cells Against Oxidative Stress. Braz J Microbiol. 2013;44(3):993-1000. PubMed PMID: 24516431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress. AU - de Sá,Rafael A, AU - de Castro,Frederico A V, AU - Eleutherio,Elis C A, AU - de Souza,Raquel M, AU - da Silva,Joaquim F M, AU - Pereira,Marcos D, Y1 - 2013/12/17/ PY - 2012/03/05/received PY - 2012/09/10/accepted PY - 2014/2/12/entrez PY - 2014/2/12/pubmed PY - 2015/2/3/medline KW - Saccharomyces cerevisiae KW - antioxidant KW - oxidative stress KW - propolis SP - 993 EP - 1000 JF - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JO - Braz J Microbiol VL - 44 IS - 3 N2 - Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1Δ, sod1Δ, gsh1Δ, gtt1Δ and gtt2Δ) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1Δ, acquired tolerance when previously treated with 25 μg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod. SN - 1678-4405 UR - https://www.unboundmedicine.com/medline/citation/24516431/Brazilian_propolis_protects_Saccharomyces_cerevisiae_cells_against_oxidative_stress_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516431/ DB - PRIME DP - Unbound Medicine ER -