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Fine mapping and metabolic and physiological characterization of the glume glaucousness inhibitor locus Iw3 derived from wild wheat.
Theor Appl Genet. 2014 Apr; 127(4):831-41.TA

Abstract

KEY MESSAGE

This research provided the first view of metabolic and physiological effect of a tissue-specific glaucousness inhibitor in wheat and laid foundation for map-based cloning of the Iw3 locus. Cuticular wax constitutes the outermost layer of plant skin, and its composition greatly impacts plant appearance and plant-environment interaction. Epicuticular wax in the upper part of adult wheat plants can form the glaucousness, which is associated with drought tolerance. In this research, we characterized a glume-specific glaucousness inhibitor, Iw3, by fine mapping, physiological, and molecular approaches. Iw3 inhibits glaucousness formation by altering wax composition. Compared to the wild type, Iw3 eliminated β-diketone, reduced 47 % primary alcohols, but increased aldehyde 400-fold and alkanes fivefold, which led to 30 % reduction of total glume wax load. Loss of the glaucousness increased cuticle permeability, suggesting an important role in drought sensitivity. Genetically, the glaucousness-inhibiting effect by Iw3 is partially dominant in a dosage-dependent manner. We localized the Iw3 locus within a 0.13-cM interval delimited by marker loci Xpsp3000 and XWL3096. Of the 53 wax genes assayed, we detected transcription changes in nine genes by Iw3, downregulation of Cer4-1 and upregulation of other five Cer4 and three KCS homologs. All these results provided initial insights into Iw3-mediated regulation of wax metabolism and paved way for in-depth characterization of the Iw3 locus and the glaucousness-related β-diketone pathway.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang J
College of Agronomy, Northwestern A&F University, Yangling, 712100, Shaanxi, China.
Li W
No affiliation info available
Wang W
No affiliation info available

MeSH

Chromosomes, PlantGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGenetic LociGenetic MarkersInbreedingPermeabilityPhenotypePhysical Chromosome MappingPlant EpidermisQuantitative Trait, HeritableTriticumWaxes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24522723

Citation

Wang, Jing, et al. "Fine Mapping and Metabolic and Physiological Characterization of the Glume Glaucousness Inhibitor Locus Iw3 Derived From Wild Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 127, no. 4, 2014, pp. 831-41.
Wang J, Li W, Wang W. Fine mapping and metabolic and physiological characterization of the glume glaucousness inhibitor locus Iw3 derived from wild wheat. Theor Appl Genet. 2014;127(4):831-41.
Wang, J., Li, W., & Wang, W. (2014). Fine mapping and metabolic and physiological characterization of the glume glaucousness inhibitor locus Iw3 derived from wild wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 127(4), 831-41. https://doi.org/10.1007/s00122-014-2260-8
Wang J, Li W, Wang W. Fine Mapping and Metabolic and Physiological Characterization of the Glume Glaucousness Inhibitor Locus Iw3 Derived From Wild Wheat. Theor Appl Genet. 2014;127(4):831-41. PubMed PMID: 24522723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fine mapping and metabolic and physiological characterization of the glume glaucousness inhibitor locus Iw3 derived from wild wheat. AU - Wang,Jing, AU - Li,Wanlong, AU - Wang,Wei, Y1 - 2014/02/13/ PY - 2013/08/10/received PY - 2014/01/03/accepted PY - 2014/2/14/entrez PY - 2014/2/14/pubmed PY - 2014/11/13/medline SP - 831 EP - 41 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 127 IS - 4 N2 - KEY MESSAGE: This research provided the first view of metabolic and physiological effect of a tissue-specific glaucousness inhibitor in wheat and laid foundation for map-based cloning of the Iw3 locus. Cuticular wax constitutes the outermost layer of plant skin, and its composition greatly impacts plant appearance and plant-environment interaction. Epicuticular wax in the upper part of adult wheat plants can form the glaucousness, which is associated with drought tolerance. In this research, we characterized a glume-specific glaucousness inhibitor, Iw3, by fine mapping, physiological, and molecular approaches. Iw3 inhibits glaucousness formation by altering wax composition. Compared to the wild type, Iw3 eliminated β-diketone, reduced 47 % primary alcohols, but increased aldehyde 400-fold and alkanes fivefold, which led to 30 % reduction of total glume wax load. Loss of the glaucousness increased cuticle permeability, suggesting an important role in drought sensitivity. Genetically, the glaucousness-inhibiting effect by Iw3 is partially dominant in a dosage-dependent manner. We localized the Iw3 locus within a 0.13-cM interval delimited by marker loci Xpsp3000 and XWL3096. Of the 53 wax genes assayed, we detected transcription changes in nine genes by Iw3, downregulation of Cer4-1 and upregulation of other five Cer4 and three KCS homologs. All these results provided initial insights into Iw3-mediated regulation of wax metabolism and paved way for in-depth characterization of the Iw3 locus and the glaucousness-related β-diketone pathway. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/24522723/Fine_mapping_and_metabolic_and_physiological_characterization_of_the_glume_glaucousness_inhibitor_locus_Iw3_derived_from_wild_wheat_ DB - PRIME DP - Unbound Medicine ER -