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Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.
BMC Microbiol. 2014 Feb 14; 14:38.BM

Abstract

BACKGROUND

The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus.

RESULTS

The detection limit of LAMP using in vitro RNA transcripts was 3.6 × 10 copies·μL⁻¹, which is as sensitive as the presently used PCR assays. However, the LAMP products could be identified as different colors with the naked eye following staining with hydroxynaphthol blue dye (HNB). No cross-reactivity with other gastroenteric viruses (rotavirus and norovirus) was observed, indicating the relatively high specificity of LAMP. The RT-LAMP method with HNB was used to effectively detect astrovirus in reclaimed water samples.

CONCLUSIONS

The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableSchool of Biological Sciences and Technology, Beijing Forestry University, Qinghua East Rd 35, P, O, Box 162, Haidian District, Beijing 100083, P, R, China. lenahe@bjfu.edu.cn.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24524254

Citation

Yang, Bo-Yun, et al. "Rapid and Sensitive Detection of Human Astrovirus in Water Samples By Loop-mediated Isothermal Amplification With Hydroxynaphthol Blue Dye." BMC Microbiology, vol. 14, 2014, p. 38.
Yang BY, Liu XL, Wei YM, et al. Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. BMC Microbiol. 2014;14:38.
Yang, B. Y., Liu, X. L., Wei, Y. M., Wang, J. Q., He, X. Q., Jin, Y., & Wang, Z. J. (2014). Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. BMC Microbiology, 14, 38. https://doi.org/10.1186/1471-2180-14-38
Yang BY, et al. Rapid and Sensitive Detection of Human Astrovirus in Water Samples By Loop-mediated Isothermal Amplification With Hydroxynaphthol Blue Dye. BMC Microbiol. 2014 Feb 14;14:38. PubMed PMID: 24524254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. AU - Yang,Bo-Yun, AU - Liu,Xiao-Lu, AU - Wei,Yu-Mei, AU - Wang,Jing-Qi, AU - He,Xiao-Qing, AU - Jin,Yi, AU - Wang,Zi-Jian, Y1 - 2014/02/14/ PY - 2013/05/12/received PY - 2014/02/06/accepted PY - 2014/2/15/entrez PY - 2014/2/15/pubmed PY - 2014/9/17/medline SP - 38 EP - 38 JF - BMC microbiology JO - BMC Microbiol. VL - 14 N2 - BACKGROUND: The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus. RESULTS: The detection limit of LAMP using in vitro RNA transcripts was 3.6 × 10 copies·μL⁻¹, which is as sensitive as the presently used PCR assays. However, the LAMP products could be identified as different colors with the naked eye following staining with hydroxynaphthol blue dye (HNB). No cross-reactivity with other gastroenteric viruses (rotavirus and norovirus) was observed, indicating the relatively high specificity of LAMP. The RT-LAMP method with HNB was used to effectively detect astrovirus in reclaimed water samples. CONCLUSIONS: The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/24524254/Rapid_and_sensitive_detection_of_human_astrovirus_in_water_samples_by_loop_mediated_isothermal_amplification_with_hydroxynaphthol_blue_dye_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-38 DB - PRIME DP - Unbound Medicine ER -