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Genetic diversity among methicillin-resistant Staphylococcus aureus isolates carrying the mecC gene in Belgium.
J Antimicrob Chemother. 2014 Jun; 69(6):1457-60.JA

Abstract

OBJECTIVES

A mecA homologue gene, named mecC, has been reported in methicillin-resistant Staphylococcus aureus (MRSA) isolates from humans and from diverse animal species. We investigated the proportion, and the phenotypic and genotypic characteristics, of mecC-carrying MRSA recovered from humans in Belgium.

METHODS

A total of 4869 S. aureus isolates, collected by the National Reference Centre from 2003 to 2012, were retrospectively analysed for the presence of mecC. The mecC-carrying MRSA isolates were tested for phenotypic resistance and the presence of toxin genes. Genotyping was performed using spa typing and multilocus sequence typing.

RESULTS

Nine S. aureus isolates, mecA negative but cefoxitin resistant (MIC 16-64 mg/L), were found to carry the mecC gene. Among these, eight showed resistance to oxacillin (MIC 4-64 mg/L). These isolates remained fully susceptible to all non-β-lactam antimicrobials. Although the proportion of mecC-carrying MRSA in Belgium was low (<1% per year), mecC-MRSA were assigned to three distinct genetic lineages corresponding to clonal complex (CC) 130, CC49 and CC1943.

CONCLUSIONS

This first Belgian nationwide analysis showed a low occurrence of mecC-MRSA. Further studies should be conducted to better understand the reservoirs and risk factors for mecC-MRSA acquisition.

Authors+Show Affiliations

National Reference Centre - Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium ariane.deplano@erasme.ulb.ac.be.National Reference Centre - Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.National Reference Centre - Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.National Reference Centre - Staphylococcus aureus, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24526516

Citation

Deplano, Ariane, et al. "Genetic Diversity Among Methicillin-resistant Staphylococcus Aureus Isolates Carrying the mecC Gene in Belgium." The Journal of Antimicrobial Chemotherapy, vol. 69, no. 6, 2014, pp. 1457-60.
Deplano A, Vandendriessche S, Nonhoff C, et al. Genetic diversity among methicillin-resistant Staphylococcus aureus isolates carrying the mecC gene in Belgium. J Antimicrob Chemother. 2014;69(6):1457-60.
Deplano, A., Vandendriessche, S., Nonhoff, C., & Denis, O. (2014). Genetic diversity among methicillin-resistant Staphylococcus aureus isolates carrying the mecC gene in Belgium. The Journal of Antimicrobial Chemotherapy, 69(6), 1457-60. https://doi.org/10.1093/jac/dku020
Deplano A, et al. Genetic Diversity Among Methicillin-resistant Staphylococcus Aureus Isolates Carrying the mecC Gene in Belgium. J Antimicrob Chemother. 2014;69(6):1457-60. PubMed PMID: 24526516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity among methicillin-resistant Staphylococcus aureus isolates carrying the mecC gene in Belgium. AU - Deplano,Ariane, AU - Vandendriessche,Stien, AU - Nonhoff,Claire, AU - Denis,Olivier, Y1 - 2014/02/12/ PY - 2014/2/15/entrez PY - 2014/2/15/pubmed PY - 2015/1/15/medline KW - MRSA KW - ST130 KW - molecular epidemiology SP - 1457 EP - 60 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 69 IS - 6 N2 - OBJECTIVES: A mecA homologue gene, named mecC, has been reported in methicillin-resistant Staphylococcus aureus (MRSA) isolates from humans and from diverse animal species. We investigated the proportion, and the phenotypic and genotypic characteristics, of mecC-carrying MRSA recovered from humans in Belgium. METHODS: A total of 4869 S. aureus isolates, collected by the National Reference Centre from 2003 to 2012, were retrospectively analysed for the presence of mecC. The mecC-carrying MRSA isolates were tested for phenotypic resistance and the presence of toxin genes. Genotyping was performed using spa typing and multilocus sequence typing. RESULTS: Nine S. aureus isolates, mecA negative but cefoxitin resistant (MIC 16-64 mg/L), were found to carry the mecC gene. Among these, eight showed resistance to oxacillin (MIC 4-64 mg/L). These isolates remained fully susceptible to all non-β-lactam antimicrobials. Although the proportion of mecC-carrying MRSA in Belgium was low (<1% per year), mecC-MRSA were assigned to three distinct genetic lineages corresponding to clonal complex (CC) 130, CC49 and CC1943. CONCLUSIONS: This first Belgian nationwide analysis showed a low occurrence of mecC-MRSA. Further studies should be conducted to better understand the reservoirs and risk factors for mecC-MRSA acquisition. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/24526516/Genetic_diversity_among_methicillin_resistant_Staphylococcus_aureus_isolates_carrying_the_mecC_gene_in_Belgium_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dku020 DB - PRIME DP - Unbound Medicine ER -