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An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles.
Analyst. 2014 Apr 07; 139(7):1726-31.A

Abstract

Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution.

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Authors+Show Affiliations

Subramanian P
Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
Motorina A
No affiliation info available
Yeap WS
No affiliation info available
Haenen K
No affiliation info available
Coffinier Y
No affiliation info available
Zaitsev V
No affiliation info available
Niedziolka-Jonsson J
No affiliation info available
Boukherroub R
No affiliation info available
Szunerits S
No affiliation info available

MeSH

AnimalsAntibodies, Anti-IdiotypicAntibodies, ImmobilizedBiosensing TechniquesBoronCattleDiamondDielectric SpectroscopyElectrodesImmunoglobulin GLimit of DetectionMicroscopy, Electron, ScanningNanoparticlesNanowiresNickelPhotoelectron SpectroscopyRabbitsSurface Properties

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24527487

Citation

Subramanian, Palaniappan, et al. "An Impedimetric Immunosensor Based On Diamond Nanowires Decorated With Nickel Nanoparticles." The Analyst, vol. 139, no. 7, 2014, pp. 1726-31.
Subramanian P, Motorina A, Yeap WS, et al. An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. Analyst. 2014;139(7):1726-31.
Subramanian, P., Motorina, A., Yeap, W. S., Haenen, K., Coffinier, Y., Zaitsev, V., Niedziolka-Jonsson, J., Boukherroub, R., & Szunerits, S. (2014). An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. The Analyst, 139(7), 1726-31. https://doi.org/10.1039/c3an02045b
Subramanian P, et al. An Impedimetric Immunosensor Based On Diamond Nanowires Decorated With Nickel Nanoparticles. Analyst. 2014 Apr 7;139(7):1726-31. PubMed PMID: 24527487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. AU - Subramanian,Palaniappan, AU - Motorina,Anastasiia, AU - Yeap,Weng Siang, AU - Haenen,Ken, AU - Coffinier,Yannick, AU - Zaitsev,Vladimir, AU - Niedziolka-Jonsson,Joanna, AU - Boukherroub,Rabah, AU - Szunerits,Sabine, PY - 2014/2/15/entrez PY - 2014/2/15/pubmed PY - 2014/9/24/medline SP - 1726 EP - 31 JF - The Analyst JO - Analyst VL - 139 IS - 7 N2 - Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/24527487/An_impedimetric_immunosensor_based_on_diamond_nanowires_decorated_with_nickel_nanoparticles_ L2 - https://doi.org/10.1039/c3an02045b DB - PRIME DP - Unbound Medicine ER -