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An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles.
Analyst. 2014 Apr 07; 139(7):1726-31.A

Abstract

Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution.

Authors+Show Affiliations

Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24527487

Citation

Subramanian, Palaniappan, et al. "An Impedimetric Immunosensor Based On Diamond Nanowires Decorated With Nickel Nanoparticles." The Analyst, vol. 139, no. 7, 2014, pp. 1726-31.
Subramanian P, Motorina A, Yeap WS, et al. An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. Analyst. 2014;139(7):1726-31.
Subramanian, P., Motorina, A., Yeap, W. S., Haenen, K., Coffinier, Y., Zaitsev, V., Niedziolka-Jonsson, J., Boukherroub, R., & Szunerits, S. (2014). An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. The Analyst, 139(7), 1726-31. https://doi.org/10.1039/c3an02045b
Subramanian P, et al. An Impedimetric Immunosensor Based On Diamond Nanowires Decorated With Nickel Nanoparticles. Analyst. 2014 Apr 7;139(7):1726-31. PubMed PMID: 24527487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles. AU - Subramanian,Palaniappan, AU - Motorina,Anastasiia, AU - Yeap,Weng Siang, AU - Haenen,Ken, AU - Coffinier,Yannick, AU - Zaitsev,Vladimir, AU - Niedziolka-Jonsson,Joanna, AU - Boukherroub,Rabah, AU - Szunerits,Sabine, PY - 2014/2/15/entrez PY - 2014/2/15/pubmed PY - 2014/9/24/medline SP - 1726 EP - 31 JF - The Analyst JO - Analyst VL - 139 IS - 7 N2 - Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/24527487/An_impedimetric_immunosensor_based_on_diamond_nanowires_decorated_with_nickel_nanoparticles_ L2 - https://doi.org/10.1039/c3an02045b DB - PRIME DP - Unbound Medicine ER -