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Tissue staining properties of lectins from the seeds of the jack fruit (Artocarpus integrifolia) and the winged bean (Psophocarpus tetragonolobus).
J Exp Pathol. 1987; 3(3):281-93.JE

Abstract

N-acetyl-D-galactosamine binding lectins from winged bean (Psophocarpus tetragonolobus) and jack fruit (Artocarpus integrifolia) were isolated, purified and conjugated with horse radish peroxidase and their tissue staining properties studied. Despite having an apparently common inhibiting sugar, the lectins showed differences in their staining properties. The lectin from the winged bean stained none of the mouse and human tissues tried even after neuraminidase treatment whereas the jack fruit lectin stained most of the untreated cells. The staining was found to be improved by the prior treatment of the cells with neuraminidase and inhibited completely by the inhibiting sugar. The differences in the staining properties of the lectins are discussed.

Authors+Show Affiliations

Vijayakumar T
Regional Cancer Centre, Trivandrum, India.
Robertson D
No affiliation info available
McIntosh D
No affiliation info available
Forrester JA
No affiliation info available

MeSH

AnimalsAstrocytomaCarcinomaFabaceaeFemaleHorseradish PeroxidaseHumansLectinsMiceMice, Inbred CBAOrgan SpecificityPlant LectinsPlants, MedicinalSeedsStaining and LabelingUterine Cervical Neoplasms

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2452865

Citation

Vijayakumar, T, et al. "Tissue Staining Properties of Lectins From the Seeds of the Jack Fruit (Artocarpus Integrifolia) and the Winged Bean (Psophocarpus Tetragonolobus)." Journal of Experimental Pathology, vol. 3, no. 3, 1987, pp. 281-93.
Vijayakumar T, Robertson D, McIntosh D, et al. Tissue staining properties of lectins from the seeds of the jack fruit (Artocarpus integrifolia) and the winged bean (Psophocarpus tetragonolobus). J Exp Pathol. 1987;3(3):281-93.
Vijayakumar, T., Robertson, D., McIntosh, D., & Forrester, J. A. (1987). Tissue staining properties of lectins from the seeds of the jack fruit (Artocarpus integrifolia) and the winged bean (Psophocarpus tetragonolobus). Journal of Experimental Pathology, 3(3), 281-93.
Vijayakumar T, et al. Tissue Staining Properties of Lectins From the Seeds of the Jack Fruit (Artocarpus Integrifolia) and the Winged Bean (Psophocarpus Tetragonolobus). J Exp Pathol. 1987;3(3):281-93. PubMed PMID: 2452865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue staining properties of lectins from the seeds of the jack fruit (Artocarpus integrifolia) and the winged bean (Psophocarpus tetragonolobus). AU - Vijayakumar,T, AU - Robertson,D, AU - McIntosh,D, AU - Forrester,J A, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 281 EP - 93 JF - Journal of experimental pathology JO - J Exp Pathol VL - 3 IS - 3 N2 - N-acetyl-D-galactosamine binding lectins from winged bean (Psophocarpus tetragonolobus) and jack fruit (Artocarpus integrifolia) were isolated, purified and conjugated with horse radish peroxidase and their tissue staining properties studied. Despite having an apparently common inhibiting sugar, the lectins showed differences in their staining properties. The lectin from the winged bean stained none of the mouse and human tissues tried even after neuraminidase treatment whereas the jack fruit lectin stained most of the untreated cells. The staining was found to be improved by the prior treatment of the cells with neuraminidase and inhibited completely by the inhibiting sugar. The differences in the staining properties of the lectins are discussed. SN - 0730-8485 UR - https://www.unboundmedicine.com/medline/citation/2452865/Tissue_staining_properties_of_lectins_from_the_seeds_of_the_jack_fruit__Artocarpus_integrifolia__and_the_winged_bean__Psophocarpus_tetragonolobus__ DB - PRIME DP - Unbound Medicine ER -