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An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis.
Liver 1988; 8(2):111-5L

Abstract

An enzyme-linked immunosorbent assay has been developed by using IgM antibodies from the acute stage as a source to capture the antigen in stools of patients with epidemic non-A, non-B (NANB) viral hepatitis. 29/69 (42.3%) of the patients and 3/9 (33.3%) contacts were positive for a suspected NANB viral antigen. However, only 1/27 (3.7%) of the negative controls drawn from amongst the patients with amoebiasis, giardiasis, hepatitis due to virus A and healthy individuals was positive for NANB antigen in the stool. The suspected NANB viral antigen was more frequently detected in stools collected between the 14th and 18th day of icteric hepatitis. The study suggests that IgM antibodies from patients with acute viral NANB hepatitis react with an antigen present in the stools of a high proportion of patients with epidemic NANB viral hepatitis. This serological test may be useful to establish the etiological diagnosis of non-A, non-B (fecal-oral) viral hepatitis. ELISA-positive stools contained 27 nm viral particles.

Authors+Show Affiliations

Department of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2452952

Citation

Gupta, H, et al. "An Enzyme-linked Immunoassay for the Possible Detection of non-A, non-B Viral Antigen in Patients With Epidemic Viral Hepatitis." Liver, vol. 8, no. 2, 1988, pp. 111-5.
Gupta H, Joshi YK, Tandon BN. An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. Liver. 1988;8(2):111-5.
Gupta, H., Joshi, Y. K., & Tandon, B. N. (1988). An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. Liver, 8(2), pp. 111-5.
Gupta H, Joshi YK, Tandon BN. An Enzyme-linked Immunoassay for the Possible Detection of non-A, non-B Viral Antigen in Patients With Epidemic Viral Hepatitis. Liver. 1988;8(2):111-5. PubMed PMID: 2452952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. AU - Gupta,H, AU - Joshi,Y K, AU - Tandon,B N, PY - 1988/4/1/pubmed PY - 1988/4/1/medline PY - 1988/4/1/entrez SP - 111 EP - 5 JF - Liver JO - Liver VL - 8 IS - 2 N2 - An enzyme-linked immunosorbent assay has been developed by using IgM antibodies from the acute stage as a source to capture the antigen in stools of patients with epidemic non-A, non-B (NANB) viral hepatitis. 29/69 (42.3%) of the patients and 3/9 (33.3%) contacts were positive for a suspected NANB viral antigen. However, only 1/27 (3.7%) of the negative controls drawn from amongst the patients with amoebiasis, giardiasis, hepatitis due to virus A and healthy individuals was positive for NANB antigen in the stool. The suspected NANB viral antigen was more frequently detected in stools collected between the 14th and 18th day of icteric hepatitis. The study suggests that IgM antibodies from patients with acute viral NANB hepatitis react with an antigen present in the stools of a high proportion of patients with epidemic NANB viral hepatitis. This serological test may be useful to establish the etiological diagnosis of non-A, non-B (fecal-oral) viral hepatitis. ELISA-positive stools contained 27 nm viral particles. SN - 0106-9543 UR - https://www.unboundmedicine.com/medline/citation/2452952/An_enzyme_linked_immunoassay_for_the_possible_detection_of_non_A_non_B_viral_antigen_in_patients_with_epidemic_viral_hepatitis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0106-9543&date=1988&volume=8&issue=2&spage=111 DB - PRIME DP - Unbound Medicine ER -