Tags

Type your tag names separated by a space and hit enter

An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis.

Abstract

An enzyme-linked immunosorbent assay has been developed by using IgM antibodies from the acute stage as a source to capture the antigen in stools of patients with epidemic non-A, non-B (NANB) viral hepatitis. 29/69 (42.3%) of the patients and 3/9 (33.3%) contacts were positive for a suspected NANB viral antigen. However, only 1/27 (3.7%) of the negative controls drawn from amongst the patients with amoebiasis, giardiasis, hepatitis due to virus A and healthy individuals was positive for NANB antigen in the stool. The suspected NANB viral antigen was more frequently detected in stools collected between the 14th and 18th day of icteric hepatitis. The study suggests that IgM antibodies from patients with acute viral NANB hepatitis react with an antigen present in the stools of a high proportion of patients with epidemic NANB viral hepatitis. This serological test may be useful to establish the etiological diagnosis of non-A, non-B (fecal-oral) viral hepatitis. ELISA-positive stools contained 27 nm viral particles.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi.

    ,

    Source

    Liver 8:2 1988 Apr pg 111-5

    MeSH

    Antigens, Viral
    Enzyme-Linked Immunosorbent Assay
    Feces
    Hepatitis Antibodies
    Hepatitis C
    Hepatitis C Antigens
    Hepatitis Viruses
    Hepatitis, Viral, Human
    Humans
    Immunoglobulin M

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    2452952

    Citation

    Gupta, H, et al. "An Enzyme-linked Immunoassay for the Possible Detection of non-A, non-B Viral Antigen in Patients With Epidemic Viral Hepatitis." Liver, vol. 8, no. 2, 1988, pp. 111-5.
    Gupta H, Joshi YK, Tandon BN. An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. Liver. 1988;8(2):111-5.
    Gupta, H., Joshi, Y. K., & Tandon, B. N. (1988). An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. Liver, 8(2), pp. 111-5.
    Gupta H, Joshi YK, Tandon BN. An Enzyme-linked Immunoassay for the Possible Detection of non-A, non-B Viral Antigen in Patients With Epidemic Viral Hepatitis. Liver. 1988;8(2):111-5. PubMed PMID: 2452952.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - An enzyme-linked immunoassay for the possible detection of non-A, non-B viral antigen in patients with epidemic viral hepatitis. AU - Gupta,H, AU - Joshi,Y K, AU - Tandon,B N, PY - 1988/4/1/pubmed PY - 1988/4/1/medline PY - 1988/4/1/entrez SP - 111 EP - 5 JF - Liver JO - Liver VL - 8 IS - 2 N2 - An enzyme-linked immunosorbent assay has been developed by using IgM antibodies from the acute stage as a source to capture the antigen in stools of patients with epidemic non-A, non-B (NANB) viral hepatitis. 29/69 (42.3%) of the patients and 3/9 (33.3%) contacts were positive for a suspected NANB viral antigen. However, only 1/27 (3.7%) of the negative controls drawn from amongst the patients with amoebiasis, giardiasis, hepatitis due to virus A and healthy individuals was positive for NANB antigen in the stool. The suspected NANB viral antigen was more frequently detected in stools collected between the 14th and 18th day of icteric hepatitis. The study suggests that IgM antibodies from patients with acute viral NANB hepatitis react with an antigen present in the stools of a high proportion of patients with epidemic NANB viral hepatitis. This serological test may be useful to establish the etiological diagnosis of non-A, non-B (fecal-oral) viral hepatitis. ELISA-positive stools contained 27 nm viral particles. SN - 0106-9543 UR - https://www.unboundmedicine.com/medline/citation/2452952/An_enzyme_linked_immunoassay_for_the_possible_detection_of_non_A_non_B_viral_antigen_in_patients_with_epidemic_viral_hepatitis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0106-9543&date=1988&volume=8&issue=2&spage=111 DB - PRIME DP - Unbound Medicine ER -