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Liquid chromatography-diode array detection-mass spectrometry for compositional analysis of low molecular weight heparins.
Anal Biochem. 2014 Apr 15; 451:35-41.AB

Abstract

Low molecular weight heparins (LMWHs) are important artificial preparations from heparin polysaccharide and are widely used as anticoagulant drugs. To analyze the structure and composition of LMWHs, identification and quantitation of their natural and modified building blocks are indispensable. We have established a novel reversed-phase high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry approach for compositional analysis of LMWHs. After being exhaustively digested and labeled with 2-aminoacridone, the structural motifs constructing LMWHs, including 17 components from dalteparin and 15 components from enoxaparin, were well separated, identified, and quantified. Besides the eight natural heparin disaccharides, many characteristic structures from dalteparin and enoxaparin, such as modified structures from the reducing end and nonreducing end, 3-O-sulfated tetrasaccharides, and trisaccharides, have been unambiguously identified based on their retention time and mass spectra. Compared with the traditional heparin compositional analysis methods, the approach described here is not only robust but also comprehensive because it is capable of identifying and quantifying nearly all components from lyase digests of LMWHs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Z
National Glycoengineering Research Center, Shandong University, Jinan 250100, China; State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Li D
National Glycoengineering Research Center, Shandong University, Jinan 250100, China; State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Sun X
National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Bai X
National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Jin L
National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
Chi L
National Glycoengineering Research Center, Shandong University, Jinan 250100, China; State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. Electronic address: lianlichi@sdu.edu.cn.

MeSH

AminoacridinesChromatography, High Pressure LiquidChromatography, Reverse-PhaseHeparin LyaseHeparin, Low-Molecular-WeightSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24530286

Citation

Wang, Zhangjie, et al. "Liquid Chromatography-diode Array Detection-mass Spectrometry for Compositional Analysis of Low Molecular Weight Heparins." Analytical Biochemistry, vol. 451, 2014, pp. 35-41.
Wang Z, Li D, Sun X, et al. Liquid chromatography-diode array detection-mass spectrometry for compositional analysis of low molecular weight heparins. Anal Biochem. 2014;451:35-41.
Wang, Z., Li, D., Sun, X., Bai, X., Jin, L., & Chi, L. (2014). Liquid chromatography-diode array detection-mass spectrometry for compositional analysis of low molecular weight heparins. Analytical Biochemistry, 451, 35-41. https://doi.org/10.1016/j.ab.2014.02.005
Wang Z, et al. Liquid Chromatography-diode Array Detection-mass Spectrometry for Compositional Analysis of Low Molecular Weight Heparins. Anal Biochem. 2014 Apr 15;451:35-41. PubMed PMID: 24530286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography-diode array detection-mass spectrometry for compositional analysis of low molecular weight heparins. AU - Wang,Zhangjie, AU - Li,Daoyuan, AU - Sun,Xiaojun, AU - Bai,Xue, AU - Jin,Lan, AU - Chi,Lianli, Y1 - 2014/02/14/ PY - 2013/11/22/received PY - 2014/02/02/revised PY - 2014/02/04/accepted PY - 2014/2/18/entrez PY - 2014/2/18/pubmed PY - 2014/11/13/medline KW - AMAC derivatization KW - Compositional analysis KW - DAD KW - ESI–MS KW - LMWH SP - 35 EP - 41 JF - Analytical biochemistry JO - Anal Biochem VL - 451 N2 - Low molecular weight heparins (LMWHs) are important artificial preparations from heparin polysaccharide and are widely used as anticoagulant drugs. To analyze the structure and composition of LMWHs, identification and quantitation of their natural and modified building blocks are indispensable. We have established a novel reversed-phase high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry approach for compositional analysis of LMWHs. After being exhaustively digested and labeled with 2-aminoacridone, the structural motifs constructing LMWHs, including 17 components from dalteparin and 15 components from enoxaparin, were well separated, identified, and quantified. Besides the eight natural heparin disaccharides, many characteristic structures from dalteparin and enoxaparin, such as modified structures from the reducing end and nonreducing end, 3-O-sulfated tetrasaccharides, and trisaccharides, have been unambiguously identified based on their retention time and mass spectra. Compared with the traditional heparin compositional analysis methods, the approach described here is not only robust but also comprehensive because it is capable of identifying and quantifying nearly all components from lyase digests of LMWHs. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/24530286/Liquid_chromatography_diode_array_detection_mass_spectrometry_for_compositional_analysis_of_low_molecular_weight_heparins_ DB - PRIME DP - Unbound Medicine ER -
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