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Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method.
Electrophoresis. 2014 Jun; 35(11):1751-5.E

Abstract

In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lin WC
Center for Nanotechnology, Department of Chemistry, Chung Yuan Christian University, Chung-Li, Taiwan.
Liu WL
No affiliation info available
Tang WY
No affiliation info available
Huang CP
No affiliation info available
Huang HY
No affiliation info available
Chin TY
No affiliation info available

MeSH

Amino AcidsAnimalsBeveragesBrain ChemistryChromatography, Micellar Electrokinetic CapillaryEmulsionsFluorescein-5-isothiocyanateFluorescenceLasersLimit of DetectionMaleMicrowavesRatsSpectrometry, Fluorescence

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24535662

Citation

Lin, Wei-Cheng, et al. "Determination of Amino Acids By Microemulsion Electrokinetic Chromatography Laser Induced Fluorescence Method." Electrophoresis, vol. 35, no. 11, 2014, pp. 1751-5.
Lin WC, Liu WL, Tang WY, et al. Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method. Electrophoresis. 2014;35(11):1751-5.
Lin, W. C., Liu, W. L., Tang, W. Y., Huang, C. P., Huang, H. Y., & Chin, T. Y. (2014). Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method. Electrophoresis, 35(11), 1751-5. https://doi.org/10.1002/elps.201300569
Lin WC, et al. Determination of Amino Acids By Microemulsion Electrokinetic Chromatography Laser Induced Fluorescence Method. Electrophoresis. 2014;35(11):1751-5. PubMed PMID: 24535662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method. AU - Lin,Wei-Cheng, AU - Liu,Wan-Ling, AU - Tang,Wei-Ying, AU - Huang,Chin-Ping, AU - Huang,Hsi-Ya, AU - Chin,Ting-Yu, Y1 - 2014/03/19/ PY - 2013/11/15/received PY - 2014/01/28/revised PY - 2014/02/10/accepted PY - 2014/2/19/entrez PY - 2014/2/19/pubmed PY - 2015/1/13/medline KW - Amino acid KW - Capillary electrophoresis KW - Laser-induced fluorescence KW - Microemulsion electrokinetic chromatography KW - Microwave-assisted derivatization SP - 1751 EP - 5 JF - Electrophoresis JO - Electrophoresis VL - 35 IS - 11 N2 - In this study, detection of 20 FITC-derivatized amino acids using an MEEKC-LIF was demonstrated. In order to achieve good separation for hydrophobic amino acids, the MEEKC method was employed and detection limits were obtained in the range of 0.32-2.2 nM, which is comparable to previous reports on amino acid analyses. Furthermore, a significant reduction in the reaction time from 1 h for conventional derivatization to 3 min for the microwave-assisted derivatization was observed and achieved, as opposed to the traditional pretreatment of real sample due to its complexity prior to the analysis of amino acid content. Finally, this microwave-assisted derivatization MEEKC-LIF method successfully determined amino acids in beverage, food, and biological samples (rat brain) with good recovery. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/24535662/Determination_of_amino_acids_by_microemulsion_electrokinetic_chromatography_laser_induced_fluorescence_method_ DB - PRIME DP - Unbound Medicine ER -

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