Tags

Type your tag names separated by a space and hit enter

Icariin decreases the expression of APP and BACE-1 and reduces the β-amyloid burden in an APP transgenic mouse model of Alzheimer's disease.
Int J Biol Sci 2014; 10(2):181-91IJ

Abstract

OBJECTIVE

The purpose of this study was to investigate the effects and pharmacological mechanisms of icariin, which is the main component in the traditional Chinese herb Epimedium, on β-amyloid (Aβ) production in an amyloid precursor protein (APP) transgenic (Tg) mouse model of Alzheimer's disease (AD).

METHODS

APPV717I Tg mice were randomly divided into a model group and icariin-treated (30 and 100 μmol/kg per day) groups. Learning-memory abilities were determined by Morris water maze and object recognition tests. Aβ contents were measured by enzyme-linked immunosorbent assays and immunohistochemistry. Amyloid plaques were detected by Congo red staining and Bielschowsky silver staining. The levels of expression of APP and β-site APP-cleaving enzyme 1 (BACE-1) were measured by western blotting and immunohistochemistry.

RESULTS

Ten-month-old Tg mice showed obvious learning-memory impairments, and significant increases in Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus. The intragastric administration of icariin to Tg mice for 6 months (from 4 to 10 months of age) improved the learning-memory abilities and significantly decreased the Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus.

CONCLUSION

Icariin reduced the Aβ burden and amyloid plaque deposition in the hippocampus of APP transgenic mice by decreasing the APP and BACE-1 levels. These novel findings suggest that icariin may be a promising treatment in patients with AD.

Authors+Show Affiliations

Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24550686

Citation

Zhang, Lan, et al. "Icariin Decreases the Expression of APP and BACE-1 and Reduces the Β-amyloid Burden in an APP Transgenic Mouse Model of Alzheimer's Disease." International Journal of Biological Sciences, vol. 10, no. 2, 2014, pp. 181-91.
Zhang L, Shen C, Chu J, et al. Icariin decreases the expression of APP and BACE-1 and reduces the β-amyloid burden in an APP transgenic mouse model of Alzheimer's disease. Int J Biol Sci. 2014;10(2):181-91.
Zhang, L., Shen, C., Chu, J., Zhang, R., Li, Y., & Li, L. (2014). Icariin decreases the expression of APP and BACE-1 and reduces the β-amyloid burden in an APP transgenic mouse model of Alzheimer's disease. International Journal of Biological Sciences, 10(2), pp. 181-91. doi:10.7150/ijbs.6232.
Zhang L, et al. Icariin Decreases the Expression of APP and BACE-1 and Reduces the Β-amyloid Burden in an APP Transgenic Mouse Model of Alzheimer's Disease. Int J Biol Sci. 2014;10(2):181-91. PubMed PMID: 24550686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Icariin decreases the expression of APP and BACE-1 and reduces the β-amyloid burden in an APP transgenic mouse model of Alzheimer's disease. AU - Zhang,Lan, AU - Shen,Cong, AU - Chu,Jin, AU - Zhang,Ruyi, AU - Li,Yali, AU - Li,Lin, Y1 - 2014/01/21/ PY - 2013/03/11/received PY - 2013/12/24/accepted PY - 2014/2/20/entrez PY - 2014/2/20/pubmed PY - 2014/11/8/medline KW - APPV717I transgenic mice KW - Alzheimer's disease KW - BACE-1 KW - Icariin KW - amyloid plaque KW - amyloid precursor protein KW - β-amyloid SP - 181 EP - 91 JF - International journal of biological sciences JO - Int. J. Biol. Sci. VL - 10 IS - 2 N2 - OBJECTIVE: The purpose of this study was to investigate the effects and pharmacological mechanisms of icariin, which is the main component in the traditional Chinese herb Epimedium, on β-amyloid (Aβ) production in an amyloid precursor protein (APP) transgenic (Tg) mouse model of Alzheimer's disease (AD). METHODS: APPV717I Tg mice were randomly divided into a model group and icariin-treated (30 and 100 μmol/kg per day) groups. Learning-memory abilities were determined by Morris water maze and object recognition tests. Aβ contents were measured by enzyme-linked immunosorbent assays and immunohistochemistry. Amyloid plaques were detected by Congo red staining and Bielschowsky silver staining. The levels of expression of APP and β-site APP-cleaving enzyme 1 (BACE-1) were measured by western blotting and immunohistochemistry. RESULTS: Ten-month-old Tg mice showed obvious learning-memory impairments, and significant increases in Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus. The intragastric administration of icariin to Tg mice for 6 months (from 4 to 10 months of age) improved the learning-memory abilities and significantly decreased the Aβ contents, amyloid plaques, and APP and BACE-1 levels in the hippocampus. CONCLUSION: Icariin reduced the Aβ burden and amyloid plaque deposition in the hippocampus of APP transgenic mice by decreasing the APP and BACE-1 levels. These novel findings suggest that icariin may be a promising treatment in patients with AD. SN - 1449-2288 UR - https://www.unboundmedicine.com/medline/citation/24550686/Icariin_decreases_the_expression_of_APP_and_BACE_1_and_reduces_the_β_amyloid_burden_in_an_APP_transgenic_mouse_model_of_Alzheimer's_disease_ L2 - http://www.ijbs.com/v10p0181.htm DB - PRIME DP - Unbound Medicine ER -